Production of bioactive compounds by solid-state fermentation of oilseed cakes

Sousa, Daniel; Salgado, José Manuel; Belo, Isabel; López, M. Cambra; Dias, Alberto Carlos Pires

doi:10.1201/9780429289798-45

Cited by 1 publication

(1 citation statement)

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“…Protease activity was expressed in units per gram of dry substrate (U/g). Total phenols were quantified using the Folin-Ciocalteau method as described by (Sousa et al, 2022) and gallic acid was used as a standard. Total phenols were expressed in mg of phenols per gram of dry substrate (mg/g).…”

Section: Methodsmentioning

confidence: 99%

Valorization of by-products from vegetable oil industries: Enzymes production by Yarrowia lipolytica through solid state fermentation

Costa

Salgado

Lopes

et al. 2022

Front. Sustain. Food Syst.

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Vegetable oil extraction generates high amounts of by-products, which are designated as oil cakes. Since the current strategies employed for oil cakes' reuse are linked with some drawbacks, identification of alternative approaches to decrease the environmental impact and promote a circular economy is of vital importance. In general, these materials are characterized by high fiber content, making them suitable to be employed in solid state fermentation (SSF). Filamentous fungi have been the microorganisms mostly applied in SSF and yeasts were applied in less extent. In the present work, three by-products from the extraction of olive, sunflower, and rapeseed oils were used as solid substrates in SSF for lipase and protease production by Yarrowia lipolytica W29. Oil cakes mixtures composition was optimized for the production of each enzyme using a simplex-centroid design of experiments. A 50% (w/w) mixture of olive cake (OC) and sunflower cake (SC) led to the highest lipase production, while a combination of the three oil cakes was most suitable for maximum protease production. Both enzymes were produced at maximum levels in a short period of 48 h. This work demonstrated that enzyme production by Y. lipolytica W29 in SSF can be modulated by the different combinations of oil cakes in the substrate mixture. Additionally, the potential of using by-products from vegetable oil industries in SSF processes was also demonstrated, showing alternative strategies for their valorization.

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Section: Methodsmentioning

confidence: 99%