Production of Bio-Based Isoprene by the Mevalonate Pathway Cassette in Ralstonia eutropha

Lee, Hyeok Won; Park, Jung Ho; Lee, Hee-Seok; Choi, Won‐Ho; Seo, Sunghwa; Anggraini, Irika Devi; Choi, Eui‐Sung

doi:10.4014/jmb.1909.09002

Cited by 11 publications

(4 citation statements)

References 33 publications

(40 reference statements)

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“…α-humulene, is of particular interest to industry, because >20 000 known natural compounds have a similar isoprenoid backbone [47]. Isoprenoids can be produced using the heterologous mevalonate (MVA) pathway in this organism [43,48]. Many isoprenoids, such as farnesane, epiisozizaene, and others, are promising candidates for aviation fuels directly or as a blending agent [49][50][51].…”

Section: Roles Of Hydrogenases In C Necatormentioning

confidence: 99%

Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2

Panich

Fong

Singer

2021

Trends in Biotechnology

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Decelerating global warming is one of the predominant challenges of our time and will require conversion of CO 2 to usable products and commodity chemicals. Of particular interest is the production of fuels, because the transportation sector is a major source of CO 2 emissions. Here, we review recent technological advances in metabolic engineering of the hydrogen-oxidizing bacterium Cupriavidus necator H16, a chemolithotroph that naturally consumes CO 2 to generate biomass. We discuss recent successes in biofuel production using this organism, and the implementation of electrolysis/artificial photosynthesis approaches that enable growth of C. necator using renewable electricity and CO 2 . Last, we discuss prospects of improving the nonoptimal growth of C. necator in ambient concentrations of CO 2 . Mitigation of Atmospheric CO 2 with BioconversionSatisfying current and future global energy demand while allowing favorable environmental outcomes will require innovative solutions in the fuel sector. Although portions of the transportation infrastructure can be electrified (such as automobile transport), energy-dense carbon-based fuels will continue to be required for aviation, long-distance trucking, rocketry, maritime shipping, and industrial operations. Petroleum-based fuels are finite, given that BP and the International Energy Agency (IEA) project that petroleum sources are likely to be depleted between 2050 and 2070 using current oil extraction technologies i-iii . Climate change caused by an increase in CO 2 (and CH 4 ) levels in Earth's atmosphere has brought cataclysmic weather events and has decimated ocean habitats in recent years, a trend that will likely continue through our lifetimes given that CO 2 is being emitted into the atmosphere at a rate of ≈32 billion tons of CO 2 per year [1-6] iv . Therefore, sustainable and economically viable bioconversion of CO 2 to fuels and commodity chemicals should be realized within the next decade. A small but significant mitigation of anthropogenic CO 2 release can be achieved by using CO 2 from atmospheric air as a feedstock to produce biofuels in microorganisms: if 10% of global aviation fuel (accounting for~2.6% of total CO 2 emissions [7]) were replaced with Sustainable Aviation Fuels (SAFs) from Cupriavidus necator at a titer of 1 g/l and assuming 90 g/l biomass accumulation [8],~500 000 tons of sustainable fuel per year could be produced from recycled CO 2 (at a carbon density of 0.8 kg/l). In addition, the accumulated biomass would sequester~80 million tons of CO 2 per year (mitigating ≈0.25% of emissions).CO 2 is an ideal feedstock for the production of biofuels because it is an inexpensive, nontoxic, and abundant starting material (≈850 billion tons of CO 2 are currently present in the atmosphere). Furthermore, CO 2 is noncompetitive with the global food supply chain. Technoeconomic analysis indicates that CO 2 -derived biofuel production could be a US$10-250 billion industry by 2030 v . However, CO 2 is dilute in the atmosphere (≈0.04% CO 2 by volume) and ...

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Section: Roles Of Hydrogenases In C Necatormentioning

confidence: 99%

Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2

Panich

Fong

Singer

2021

Trends in Biotechnology

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“…Either for efficient production of foreign proteins or successful construction of long metabolic pathways, selection of endogenous or exogenous genes from suitable organisms is important. Codon optimization is an effective strategy for efficient expression of heterologous genes, because the GC content of C. necator genome is about 66% and much higher than that of most organisms [ 36 , 102 , 103 ]. Differences in GC content may impair transcriptional and translational efficiencies, limiting the enzyme activities and thus production yields.…”

Section: Heterologous Gene Expression Elementsmentioning

confidence: 99%

“…Isoprene (C5) is widely used in the synthetic rubber industry and fuel additives, whose precursor is DMAPP. Lee et al introduced MVA genes and an isoprene synthase gene ( ispS ) to C. necator H16 and obtained 3.8 µg/L isoprene through codon and promoter optimization [ 102 ]. Monoterpenoids (C10), such as limonene, have strong fragrance and biological activity, and are important raw materials in pharmaceutical, food, and cosmetic industries.…”

Section: Synthetic Biology Applications Of C Necator H16mentioning

confidence: 99%

Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization

Pan

Wang

et al. 2021

Biotechnol Biofuels

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Background CO2 valorization is one of the effective methods to solve current environmental and energy problems, in which microbial electrosynthesis (MES) system has proved feasible and efficient. Cupriviadus necator (Ralstonia eutropha) H16, a model chemolithoautotroph, is a microbe of choice for CO2 conversion, especially with the ability to be employed in MES due to the presence of genes encoding [NiFe]-hydrogenases and all the Calvin–Benson–Basham cycle enzymes. The CO2 valorization strategy will make sense because the required hydrogen can be produced from renewable electricity independently of fossil fuels. Main body In this review, synthetic biology toolkit for C. necator H16, including genetic engineering vectors, heterologous gene expression elements, platform strain and genome engineering, and transformation strategies, is firstly summarized. Then, the review discusses how to apply these tools to make C. necator H16 an efficient cell factory for converting CO2 to value-added products, with the examples of alcohols, fatty acids, and terpenoids. The review is concluded with the limitation of current genetic tools and perspectives on the development of more efficient and convenient methods as well as the extensive applications of C. necator H16. Conclusions Great progress has been made on genetic engineering toolkit and synthetic biology applications of C. necator H16. Nevertheless, more efforts are expected in the near future to engineer C. necator H16 as efficient cell factories for the conversion of CO2 to value-added products.

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“…However, the supply of crude oil for isoprene extraction is declining as a result of trends in the petroleum industry toward using lighter hydrocarbon feedstock streams for cracking [4]. As an alternative, biobased isoprene can be successfully synthesized by microbial engineering using the mevalonate (MVA) and 1‐deoxy‐ d ‐xylulose‐5‐phosphate (DXP) biosynthetic pathways [5–7].…”

mentioning

confidence: 99%

Engineered Escherichia coli strains as platforms for biological production of isoprene

et al. 2020

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Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21, BL21 (DE3), Rosetta, and BW25113. Here, we tested four groups of E. coli strains for improved isoprene production, including K‐12 (DH5α, BW25113, W3110, MG1655, XL1‐Blue, and JM109), B [Rosetta (DE3), BL21, and BL21 (DE3)], Crooks C, and Waksman W strains. The isoprene productivity of BL21 and MG1655 was remarkably higher than that of the others in 5‐L fermentation, and scale‐up fermentation (300 L) of BL21 was successfully performed. This system shows potential for biobased production of fuel and volatile compounds in industrial applications.

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Production of Bio-Based Isoprene by the Mevalonate Pathway Cassette in Ralstonia eutropha

Cited by 11 publications

References 33 publications

Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2

Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2

Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization

Engineered Escherichia coli strains as platforms for biological production of isoprene

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