Although thymus-matured T cells are not necessary for in vivo B cell responses to a class of antigens designated T-independent (TI) 1 (1, 2), we wished to determine whether T cells could influence the nature of the antibody produced. It was our hypothesis that in a TI antigen system in which there is no obligate need for carrierspecific helper T cell triggering signals, T cells may still exert subtle regulatory effects on the B cell antibody response via a distinct population of Ig-specific regulatory cells (3-9). If this were indeed the case, such a system might be ideal for studying the regulatory effects of Ig-speeific T cells independently of carrier-specific T cells. The characteristics of Ig-specific T cell interactions with B cells could thus be elucidated and compared with the known characteristics of carrier-specific T cell-B cell interactions.In testing for T cell influences on B cell responses to TI antigens, our emphasis has been to search for possible effects of isotype-specific T cells. Our approach has been to determine whether T cells could differentially regulate the levels of the various isotypes of antibody made by the B cell population responding to a subset of TI antigens, designated type 2 TI (TI-2) antigens. These antigens are characterized by their lack of polyclonal activating activity and their inability to stimulate B cells from neonatal mice or mice with the CBA/N immune defect (10). We here demonstrate that the in vivo B cell response to TI-2 antigens can be influenced by T lymphocytes and that this influence is restricted to only some of the Ig isotypes produced, namely the IgG2a, and, to a lesser extent, the IgG2b subclasses.
Materials and MethodsAnimals. C57BLI0/ScN nu/nu, C57BL10/ScN nu/+, C57BL/KaLwN, and (CBA/N × C57BL/6)F1 mice were obtained from the Small Animal Section, Division of Research Services, National Institutes of Health, Bethesda, Md. Age matched nu/nu and nu/+ male and female mice from 2-4 mo of age were used in each experiment.Antigens and lmmunizations. Trinitrophenyl-aminoethylcarbamylmethyl (TNP-AECM)-Ficoll (mol wt = 400,000; 55 mol TNP/mol Ficoll) was purchased from Biosearch (San Rafael, Calif.).