“…1 ) Synthesis of the enzyme· is repressed by aspartate and glutamate,l) and the activity is feedback-inhibited by aspartate, and furthermore synergistically, by both aspartate and 2-oxoglutarate, a precursor of glutamate. 2 -S ) On the other hand, acetyl-CoA not only activates the enzyme 2 ,3) but also restrains its aspartate inhibition.2) Nevertheless, aspartate- 6 ) or lysine-7 ,S) overproduction by B. flavum mutants was found to be remarkably promoted by defects of pyruvate kinase or a reduction of pyruvate dehydrogenase activity, although these alterations seemed to decrease the intracellular level of acetyl-CoA. These findings suggest that another activator of the enzyme, fructose 1,6-bisphosphate (FBP), which has been reported with enteric bacteria, 9 -11 ) plays a physiologically important role in B.flavum.…”