Production of artificial synthetic spidroin gene 4S-transgenic cloned sheep embryos using somatic cell nuclear transfer

Li, Hao; Chen, Shengnan; Piao, Shan-Hua; Tie-zhu, AN; Wang, Chunsheng

doi:10.1080/10495398.2020.1737098

Cited by 13 publications

(5 citation statements)

References 50 publications

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“…Purified protein powder was obtained from such milk using special filtration techniques [ 89 ]. However, attempts to express recombinant spider silk proteins in sheep hair follicles have been unsuccessful [ 90 ].…”

Section: Recombinant Production Of Spider Silkmentioning

confidence: 99%

Review of Spider Silk Applications in Biomedical and Tissue Engineering

Branković,

Zivic,

Grujovic

et al. 2024

Biomimetics

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This review will present the latest research related to the production and application of spider silk and silk-based materials in reconstructive and regenerative medicine and tissue engineering, with a focus on musculoskeletal tissues, and including skin regeneration and tissue repair of bone and cartilage, ligaments, muscle tissue, peripheral nerves, and artificial blood vessels. Natural spider silk synthesis is reviewed, and the further recombinant production of spider silk proteins. Research insights into possible spider silk structures, like fibers (1D), coatings (2D), and 3D constructs, including porous structures, hydrogels, and organ-on-chip designs, have been reviewed considering a design of bioactive materials for smart medical implants and drug delivery systems. Silk is one of the toughest natural materials, with high strain at failure and mechanical strength. Novel biomaterials with silk fibroin can mimic the tissue structure and promote regeneration and new tissue growth. Silk proteins are important in designing tissue-on-chip or organ-on-chip technologies and micro devices for the precise engineering of artificial tissues and organs, disease modeling, and the further selection of adequate medical treatments. Recent research indicates that silk (films, hydrogels, capsules, or liposomes coated with silk proteins) has the potential to provide controlled drug release at the target destination. However, even with clear advantages, there are still challenges that need further research, including clinical trials.

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Section: Recombinant Production Of Spider Silkmentioning

confidence: 99%

Review of Spider Silk Applications in Biomedical and Tissue Engineering

Branković,

Zivic,

Grujovic

et al. 2024

Biomimetics

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“…Recently, there has been a report of incorporating rSSP into sheep embryos which was targeted for expression in the hair follicle ( Li et al, 2021 ). Unfortunately, none of the implanted embryos resulted in live fetuses.…”

Section: Recombinant Spider Silk Expression Systemsmentioning

confidence: 99%

Bioengineering of spider silks for the production of biomedical materials

Bittencourt

Oliveira

Michalczechen-Lacerda

et al. 2022

Front. Bioeng. Biotechnol.

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Spider silks are well known for their extraordinary mechanical properties. This characteristic is a result of the interplay of composition, structure and self-assembly of spider silk proteins (spidroins). Advances in synthetic biology have enabled the design and production of spidroins with the aim of biomimicking the structure-property-function relationships of spider silks. Although in nature only fibers are formed from spidroins, in vitro, scientists can explore non-natural morphologies including nanofibrils, particles, capsules, hydrogels, films or foams. The versatility of spidroins, along with their biocompatible and biodegradable nature, also placed them as leading-edge biological macromolecules for improved drug delivery and various biomedical applications. Accordingly, in this review, we highlight the relationship between the molecular structure of spider silk and its mechanical properties and aims to provide a critical summary of recent progress in research employing recombinantly produced bioengineered spidroins for the production of innovative bio-derived structural materials.

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“…A recent study by Li and colleagues reported the successful development of transgenic sheep embryos containing a recombinant spidroin gene successfully integrated in the genome [59]. Rather than secreting recombinant proteins via the mammary gland, the study aimed to produce Trends in Biotechnology transgenic sheep capable of producing recombinant spidroins via their abundant hair and wool follicles as a component of hair.…”

Section: Mammalian Cell Lines and Transgenic Animalsmentioning

confidence: 99%

“…However, while embryo transplantation was performed and pregnancy was observed, no offspring were produced. Consequently, viability of spidroin production within hair is yet to be demonstrated [59].…”

Section: Mammalian Cell Lines and Transgenic Animalsmentioning

confidence: 99%

Host Systems for the Production of Recombinant Spider Silk

Whittall

Baker

Breitling

et al. 2021

Trends in Biotechnology

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Spider silk is renowned for its impressive mechanical properties. It is one of the strongest known biomaterials, possessing mechanical properties that outmatch both steel and Kevlar. However, the farming of spiders for their silk is unfeasible. Consequently, production of recombinant spider silk proteins (spidroins) in more amenable hosts is an exciting field of research. For large-scale production to be viable, a heterologous silk production system that is both highly efficient and cost effective is essential. Genes encoding recombinant spidroin have been expressed in bacterial, yeast, insect, and mammalian cells, in addition to many other platforms. This review discusses the recent advances in exploiting an increasingly diverse range of host platforms in the heterologous production of recombinant spidroins. Highlights Recombinant spidroins of comparable size and mechanical function to native silk proteins have been successfully produced by engineered E. coli. Recombinant spidroins have been expressed in transgenic plants, rice, and alfalfa. These expression platforms can potentially offer increased industrialscale economic viability over alternative host systems.

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Production of artificial synthetic spidroin gene 4S-transgenic cloned sheep embryos using somatic cell nuclear transfer

Cited by 13 publications

References 50 publications

Review of Spider Silk Applications in Biomedical and Tissue Engineering

Review of Spider Silk Applications in Biomedical and Tissue Engineering

Bioengineering of spider silks for the production of biomedical materials

Host Systems for the Production of Recombinant Spider Silk

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