Production of antiviral vaccine antigens using a silkworm-baculovirus expression system

Kusakabe, Takahiro

doi:10.1016/j.jphs.2023.01.002

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Expressing proteins in silkworms via the baculovirus expression vector platform offers several advantages, including high expression levels, low costs, and the capacity to produce multiple proteins in large quantities, as well as the ability to perform co- and post-translational modifications characteristic of mammalian cells ( 2 ). This platform increases production and enhances the supply of various medium-scale recombinant proteins and has led to the rise of a promising vaccine production technology ( 3 ). In addition, it incorporates piggyBac transposon as well as genome-editing technologies to facilitate advanced utilization of transgenic silkworms ( 4–6 ).…”

Section: Introductionmentioning

confidence: 99%

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Evaluating bio-physicochemical properties of raw powder prepared from whole larvae containing liquid silk of the domestic silkworm

Hashimoto,

Yamazaki,

Uehara

et al. 2024

Front. Nutr.

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The domestic silkworm, Bombyx mori, has been widely used in silk production for centuries. It is also used as a bioreactor by the textile and pharmaceutical industries to mass produce recombinant bioactive proteins containing silk-based materials. Furthermore, silkworms are well-known as a source of food and have also been orally administered to prevent and treat several human disorders. In this study, we aimed to investigate the inherent bio-physicochemical properties of edible silkworms to accurately evaluate their clinical and nutritional potential. We prepared raw powder from whole larvae of silkworm. The yield rate of the powder derived from dried larvae was almost 100% (98.1–99.1% in replicates). As “percentage yield” translates to “Budomari” in Japanese, this raw powder was named “B100rw.” We further prepared B100dn that was denatured through autoclaving. Thereafter, we examined whether B100rw sustained the original bio-physicochemical properties by comparing it with B100dn. There was no significant difference in nutritional content between B100rw and B100dn. B100rw contained proteins derived from silkworm larvae and mulberry leaves, whereas the proteins of B100dn were mostly degraded. On measuring the enzymatic activity of both powders using trehalase as an indicator enzyme, B100rw was found to maintain trehalase activity. B100rw also maintained a random coil conformation, similar to that of liquid silk. This suggested that B100rw sustained the unique bio-physicochemical properties of living larvae. These findings may facilitate the development of novel food products or orally administered vaccines.

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Section: Introductionmentioning

confidence: 99%

“…All values are based on 100 g of powder. 2 kcal = 4 × protein content + 9 × fat content + 4 × carbohydrate content 3. Carbohydrate content = 100 − (fat content + protein content + ash content + moisture content).…”

mentioning

confidence: 99%

Evaluating bio-physicochemical properties of raw powder prepared from whole larvae containing liquid silk of the domestic silkworm

Hashimoto,

Yamazaki,

Uehara

et al. 2024

Front. Nutr.

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show abstract

“…This suggested that expression levels, low costs, and the capacity to produce multiple proteins in large quantities, as well as the ability to perform co-and post-translational modifications characteristic of mammalian cells (2). This platform increases production and enhances the supply of various medium-scale recombinant proteins and has led to the rise of a promising vaccine production technology (3). In addition, it incorporates piggyBac transposon as well as genome-editing technologies to facilitate advanced utilization of transgenic silkworms (4)(5)(6).…”

mentioning

confidence: 99%

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Drug Development Using Human iPSC-Derived Cardiomyocytes and Future Perspectives

Kawagishi,

Yanagida,

Kanda

2024

Reference Module in Biomedical Sciences

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Production of antiviral vaccine antigens using a silkworm-baculovirus expression system

Cited by 4 publications

References 6 publications

Evaluating bio-physicochemical properties of raw powder prepared from whole larvae containing liquid silk of the domestic silkworm

Evaluating bio-physicochemical properties of raw powder prepared from whole larvae containing liquid silk of the domestic silkworm

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Drug Development Using Human iPSC-Derived Cardiomyocytes and Future Perspectives

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