Production of Antisera Against Plasma Lipoprotein Fraction.

Grant, Wilson C.; Berger, H.

doi:10.3181/00379727-86-21231

Cited by 7 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunochemical techniques have been used extensively in studies on the chemistry and metabolism of serum lipoproteins. Several investigators (1)(2)(3)(4)(5)(6)(7)(8) have produced antibodies against normal human lipoproteins by the injection of purified lipoproteins into rabbits, or into chickens (9). The antigen-antibody reaction between lipoproteins and their antisera has been demonstrated by precipitation (1-3, 5, 8), complement fixation (2,3), gel diffusion (1)(2)(3)(4)(6)(7)(8), and immuno-electrophoresis (6,10).…”

mentioning

confidence: 99%

Studies on the Immunochemistry of Human Low Density Lipoproteins Utilizing an Hemagglutination Technique

Briner

Riddle

Cornwell

1959

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Hemagglutination is a specific and sensitive technique for investigating the purity of lipoproteins and the immunologic relationships between low density lipoprotein fractions. The Sf 10–400 and Sf 3–9 lipoprotein fractions, isolated from human serum by dextran sulfate-density gradient centrifugation procedure and repurified by centrifugation appeared to contain only lipoprotein antigens since these fractions did not stimulate the production of antibodies against other serum proteins. Cross-absorption experiments with lipoproteins carried on "tanned" cells demonstrated that the Sf 3–9 lipoprotein fraction contains all the antigenic components of the Sf 10–400 lipoprotein fraction together with additional antigenic components not found in the Sf 10–400 lipoprotein fraction. Thus Sf 3–9 and Sf 10–400 lipoprotein fractions are immunologically similar but not identical. Low density lipoproteins contain no antigens in common with the high density lipoproteins. An Sf 3–9 antiserum can be used to detect both Sf 3–9 and Sf 10–400 antigens. The Sf 3–9 lipoprotein fraction used as an antigen will detect antibodies against both Sf 3–9 and Sf 10–400 lipoprotein fractions. The Sf 3–9 and Sf 10–400 antisera did not contain immune antibodies against erythrocytes of the different blood groups or against sheep, guinea pig, dog, calf, pig, horse, and chicken erythrocytes. Normal subjects and subjects with recent myocardial infarctions had no circulating autoantibodies against the Sf 3–9 and Sf 10–400 lipoprotein fractions.

show abstract