Production of Antibodies against Hepatitis C Virus Envelope Glycoprotein E2- A Potential Vaccine Against HCV Infection

Afshan, Gul; Zulfiqar, Soumble; Mehboob, Sumaira; Khan, Muhammad Tahir; Shakoori, A. R.

doi:10.17582/journal.pjz/2019.51.6.2311.2322

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…As is evident by previous coronaviruses associated pandemics and other viral diseases, a highly specific, sensitive and easily deployable diagnostic tool is critical for identification, tracing, rationalizing control measures and documentation of symptomatic and asymptomatic carriers [ 7 , 8 , 9 , 10 , 11 , 12 ]. Additionally, due to the unavailability of the registered vaccines or effective therapeutics, rapid and reliable diagnostics are of paramount importance to curtail SARS-CoV-2 infection.…”

Section: Introductionmentioning

confidence: 99%

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (AI-LAMP) for Rapid Detection of SARS-CoV-2

Rohaim

Clayton

Şahin

et al. 2020

Viruses

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Until vaccines and effective therapeutics become available, the practical solution to transit safely out of the current coronavirus disease 19 (CoVID-19) lockdown may include the implementation of an effective testing, tracing and tracking system. However, this requires a reliable and clinically validated diagnostic platform for the sensitive and specific identification of SARS-CoV-2. Here, we report on the development of a de novo, high-resolution and comparative genomics guided reverse-transcribed loop-mediated isothermal amplification (LAMP) assay. To further enhance the assay performance and to remove any subjectivity associated with operator interpretation of results, we engineered a novel hand-held smart diagnostic device. The robust diagnostic device was further furnished with automated image acquisition and processing algorithms and the collated data was processed through artificial intelligence (AI) pipelines to further reduce the assay run time and the subjectivity of the colorimetric LAMP detection. This advanced AI algorithm-implemented LAMP (ai-LAMP) assay, targeting the RNA-dependent RNA polymerase gene, showed high analytical sensitivity and specificity for SARS-CoV-2. A total of ~200 coronavirus disease (CoVID-19)-suspected NHS patient samples were tested using the platform and it was shown to be reliable, highly specific and significantly more sensitive than the current gold standard qRT-PCR. Therefore, this system could provide an efficient and cost-effective platform to detect SARS-CoV-2 in resource-limited laboratories.

show abstract

Section: Introductionmentioning

confidence: 99%

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (AI-LAMP) for Rapid Detection of SARS-CoV-2

Rohaim

Clayton

Şahin

et al. 2020

Viruses

View full text Add to dashboard Cite

show abstract

“…As evident by previous coronaviruses associated pandemics and other viral diseases, a highly specific, sensitive and easily deployable diagnostic is critical for the identification, tracing, rationalizing control measures, documentation of symptomatic and asymptomatic carriers [7][8][9][10][11][12]. Additionally, due to the unavailability of the registered vaccines or effective therapeutics, rapid and reliable diagnostics are of paramount importance to curtail SARS-CoV-2 infection.…”

Section: Introductionmentioning

confidence: 99%

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid and Reliable Detection of SARS-CoV-2

Rohaim

Clayton

Şahin

et al. 2020

Preprint

View full text Add to dashboard Cite

Until vaccines and effective therapeutics become available, the practical way to transit safely out of the current lockdown may include the implementation of an effective testing, tracing and tracking system. However, this requires a reliable and clinically validated diagnostic platform for the sensitive and specific identification of SARS-CoV-2. Here, we report on the development of a de novo, high-resolution and comparative genomics guided reverse-transcribed loop-mediated isothermal amplification (LAMP) assay. To further enhance the assay performance and to remove any subjectivity associated with operator interpretation of result, we engineered a novel hand-held smart diagnostic device. The robust diagnostic device was further furnished with automated image acquisition and processing algorithms, and the collated data was processed through artificial intelligence (AI) pipelines to further reduce the assay run time and the subjectivity of the colorimetric LAMP detection. This advanced AI algorithm-implemented LAMP (ai-LAMP) assay, targeting the RNA-dependent RNA polymerase gene, showed high analytical sensitivity and specificity for SARS-CoV-2. A total of ~200 coronavirus disease (CoVID-19)-suspected patient samples were tested using the platform and it was shown to be reliable, highly specific and significantly more sensitive than the current gold standard qRT-PCR. The system could provide an efficient and cost-effective platform to detect SARS-CoV-2 in resource-limited laboratories.

show abstract

Production of Antibodies against Hepatitis C Virus Envelope Glycoprotein E2- A Potential Vaccine Against HCV Infection

Cited by 2 publications

References 8 publications

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (AI-LAMP) for Rapid Detection of SARS-CoV-2

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (AI-LAMP) for Rapid Detection of SARS-CoV-2

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid and Reliable Detection of SARS-CoV-2

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