Production of Anomalous Structures in Quercus rubra L. Callus Cultures

Seckinger, Gary R.; McCown, Brent H.; Struckmeyer, Β. Esther

doi:10.2307/2442244

Cited by 11 publications

(3 citation statements)

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“…NAA/BAP combination is commonly used for callus induction. Thus, in Quercus rubra, Seckinger et al [35] [36] obtained a dense, yellow-white, nodular callus developing at the periphery of the stem segments grown for one month on a modified MS medium containing these two growth regulators.…”

Section: L El Kbiach Et Almentioning

confidence: 99%

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Callogenesis of Cork Oak (Quercus suber L.) through In Vitro Culture of Nodes and Internodes

Kbiach¹,

Bouzdoudi²,

Saidi³

et al. 2017

AJPS

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The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 µM of 6-Benzylaminopurine (BAP), 7 µM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting; small white embryos appear on the surface of calluses.

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Section: L El Kbiach Et Almentioning

confidence: 99%

“…Previously, Seckinger et al [35] observed small nodules on the periphery of calluses produced by internodes of young Quercus rubra plants, which they de-scribed as organoids. But these formations, entirely parenchymatous, had no meristematic zone and showed no further development.…”

Section: L El Kbiach Et Almentioning

confidence: 99%

Callogenesis of Cork Oak (Quercus suber L.) through In Vitro Culture of Nodes and Internodes

Kbiach¹,

Bouzdoudi²,

Saidi³

et al. 2017

AJPS

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show abstract

“…Callus cultures obtained from male catkins using BA or 2,4-D did not regenerate embryos in NRO (Gingas 1991). Seckinger et al (1979) reported the development of normal roots on organoids initiated from callus cultures on medium containing NAA and BA, although no shoots were observed.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Vengadesan

Pijut

2009

Plant Cell Tiss Organ Cult

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A somatic embryogenesis protocol for plant regeneration of northern red oak (Quercus rubra) was established from immature cotyledon explants. Embryogenic callus cultures were induced on Murashige and Skoog medium (MS) containing 3% sucrose, 0.24% Phytagel TM , and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) after 4 weeks of culture in darkness. A higher response (66%) of embryogenic callus was induced on 0.45 lM 2,4-D. Higher numbers of globular-(31), heart-(17), torpedo-(12), and cotyledon-stage (8) embryos per explant were obtained by culturing embryogenic callus on MS with 3% sucrose, 0.24% Phytagel TM , and devoid of growth regulators after 8 weeks culture in darkness. Continuous sub-culturing of embryogenic callus on medium containing 2,4-D yielded only compact callus. Desiccation of embryos for 3 days in darkness at 25 ± 2°C followed by cold storage at 4°C in darkness for 8 weeks favored embryo germination and development of plantlets. Cotyledon-stage embryos subjected to desiccation and chilling treatment cultured on MS with 3% sucrose, 0.24 Phytagel TM , 0.44 lM 6-benzylaminopurine (BA), and 0.29 lM gibberellic acid germinated at a higher frequency (61%) than with 0.44 lM BA alone and control cultures. Germinated plantlets developed a shoot and root, were acclimatized successfully, and maintained in a growth room for plantlet development.

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Quercus spp. (Oak): In Vitro Culture and Production of Tannins

Favre¹,

Scalbert

Penhoat

1993

Biotechnology in Agriculture and Forestry

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Production of Anomalous Structures in Quercus rubra L. Callus Cultures

Cited by 11 publications

References 0 publications

Callogenesis of Cork Oak (<i>Quercus suber</i> L.) through <i>In Vitro</i> Culture of Nodes and Internodes

Callogenesis of Cork Oak (<i>Quercus suber</i> L.) through <i>In Vitro</i> Culture of Nodes and Internodes

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Quercus spp. (Oak): In Vitro Culture and Production of Tannins

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Production of Anomalous Structures in Quercus rubra L. Callus Cultures

Cited by 11 publications

References 0 publications

Callogenesis of Cork Oak (&lt;i&gt;Quercus suber&lt;/i&gt; L.) through &lt;i&gt;In Vitro&lt;/i&gt; Culture of Nodes and Internodes

Callogenesis of Cork Oak (&lt;i&gt;Quercus suber&lt;/i&gt; L.) through &lt;i&gt;In Vitro&lt;/i&gt; Culture of Nodes and Internodes

Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.)

Quercus spp. (Oak): In Vitro Culture and Production of Tannins

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Product

Resources

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Callogenesis of Cork Oak (<i>Quercus suber</i> L.) through <i>In Vitro</i> Culture of Nodes and Internodes

Callogenesis of Cork Oak (<i>Quercus suber</i> L.) through <i>In Vitro</i> Culture of Nodes and Internodes