Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers

Brandelli, Adriano; Riffel, Alessandro

doi:10.2225/vol8-issue1-fulltext-6

Cited by 52 publications

(30 citation statements)

References 18 publications

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“…This confirms that high substrate concentration reduced keratinase production (Fig. 3) as previously reported by Brandelli and Riffel (2005), Wang and Shih (1999) and Lin and Yin (2010). As approximately 40% of total cost of enzyme production is due to the cost of growth substrates (Kumar and Parrack 2003); the use of low cost and readily available feather meal or chicken -11 -feather substrate will enable the sustainable production of strain N22 keratinase on an industrial scale.…”

Section: Optimisation Of Keratinase Production and Characterisation Osupporting

confidence: 91%

“…Rather, in most reported feather degradation studies, the feather substrates are usually young, downy white feathers (Brandelli and Riffel 2005;Cao et al 2008;Zhang et al 2009). Notably, melanised feathers are generally more resistant to enzymatic attack than white feathers due to the presence of the pigment melanin that binds to and inhibits keratinases (Goldstein et al 2004;Gunderson et al 2008) as well as other enzymes (Kuo and Alexander 1967).…”

Section: Degradation Of Melanised Feathermentioning

confidence: 99%

“…Feather degradation by keratinases has been mostly performed in the presence of suitable reducing agents such as live cells or chemical reductants (Brandelli and Riffel 2005;Ramnani and Gupta 2007;Liang et al 2010). Cell-free keratinases of Streptomyces BA 7 (Korkmaz et al 2003), Streptomyces S7 (Tatineni et al 2008) and B. licheniformis ER-15 (Tiwary and Gupta 2010) have been reported to degrade feather in 24 h, 97 h and 12 h respectively.…”

Section: Degradation Of Melanised Feathermentioning

confidence: 99%

See 2 more Smart Citations

Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather

Okoroma

Garelick

Abiola

et al. 2012

International Biodeterioration & Biodegradation

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Significant amount of keratins in the form of feather, hair, hoof and horn are generated annually by the livestock industry. Keratinases are increasingly important in the reprocessing and environmental pollution control of keratin wastes. The aim of this study is to isolate a microbial strain of high keratinase activity and to evaluate its feather degrading potential.Thirty-two keratin degrading microbial strains from farmyard wastes and primary effluent were isolated using a selective medium containing feather meal at 30, 37 and 50 °C. One of the isolates, which demonstrated the highest keratinolytic activity (11.00 ± 0.71 Uml -1 ) was identified as a species of Bacillus licheniformis based on the 16S rDNA analysis, designated as strain N22 and deposited in a culture collection. Optimum keratinase production by this bacterium was achieved in 32 h using a minimum growth medium containing 1.1% (w/v) feather meal at 50 °C and pH 8.

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Section: Optimisation Of Keratinase Production and Characterisation Osupporting

confidence: 91%

Section: Degradation Of Melanised Feathermentioning

confidence: 99%

Section: Degradation Of Melanised Feathermentioning

confidence: 99%

See 1 more Smart Citation

Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather

Okoroma

Garelick

Abiola

et al. 2012

International Biodeterioration & Biodegradation

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“…Keratinous waste is widely generated by various industries. Feathers are byproducts of poultry production that can provide suitable conditions for the growth of anaerobic or aerobic bacteria if not removed from the environment (7,8). Thus, it is necessary to investigate the production of keratinase with particular emephisis on enzyme extraction technology at industrial scale.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island

2018

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Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-producing bacteria can be a valuable solution. The present study aimed for isolation and molecular identification of keratinase-producing bacteria in Qeshm Island and Peyposht village in Iran.Methods: Water and sludge samples from the Qeshm Island and Peyposht village were collected. The bacteria isolates were screened for keratinase production using the Lowry method. Effect of pH and temperature was assessed on the production of keratinase and on the growth of the isolates. Colony-polymerase chain reaction was used for molecular identification of the isolates.Results: Bacillus berevis and Enterobacter cloacae were isolated in this study. Keratinase production in B. berevis was highest at pH 7.5 and 35 °C. In addition, the highest level of enzyme production by E. cloacae was observed at pH 7 and 37 °C.Conclusion: It seems that the bacterial strains isolated from sludge in the study area have relatively favorable keratinase production capacity.

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“…Its extracellular keratinase is a metalloprotease with great potential for biotechnological applications (Riffel et al, 2003). Another work describes the effect of temperature, initial pH and substrates on keratinase production by Chryseobacterium sp., kr6 during growth on native feathers (Brandelli and Riffel, 2005). More recently, Kansoh et al (2009) isolated two bacterial strains producing keratinase from Egypt soil.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Molecular Characterization of a New Local Keratinase Producing Pseudomomanas sp., MS21

Tork¹,

Aly²,

Nawar³

2009

Asian J. of Biotechnology

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This study aimed to isolate and identify a new local bacterial strain, able to completely degrade keratin-rich wastes into soluble and useful materials which can be used in many proposes. Bacterial keratinases are of particular interest because of their action on insoluble keratin substrates and generally on a broad range of protein substrates. These enzymes have been studied for de-hairing processes in the leather industry and hydrolysis of feather and keratin. Samples from poultry industry wastes, soil, water, fodder and feather were collected from different places in Jeddah, Saudi Arabia. Each sample was plated on feather meal agar plates containing 5 g LG feather as the sole carbon and nitrogen source and 1 the obtained colonies were selected, purified and their growth were detected on skimmed milk agar and feather meal broth media. The well grown isolates on feather meal agar which producing the largest clearing zone on skimmed milk plate were selected for keratinase assays. Out of 23 bacterial isolates, 7 isolates were selected. The best keratinase producing bacterium kera MS21 was selected and identified based on morphological, physiological and some biochemical characteristics. It was recorded as a species belonging to the genus Pseudomonas and identified as Pseudomonas sp. The results of identification were confirmed by 16S rDNA studies. Precipitation and purification of the keratinase enzyme in addition to factors affecting enzyme activity were studied. The enzyme molecular weight was determined to be of 30 KDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The optimum temperature and pH were determined to be 37°C and pH 8.0, respectively. The effect of some proteases inhibitors and activators were also studied.

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Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers

Cited by 52 publications

References 18 publications

Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather

Identification and characterisation of a Bacillus licheniformis strain with profound keratinase activity for degradation of melanised feather

Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island

Biochemical and Molecular Characterization of a New Local Keratinase Producing Pseudomomanas sp., MS21

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