Production of an endoglucanase by the shipworm bacterium, Teredinobacter turnirae

Ahuja, Sanjeev; Ferreira, Gisela; Moreira, A.

doi:10.1007/s10295-004-0113-1

Cited by 22 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and their release in solid state or submerged fermentation however, the mechanism of action is still not clear [35,36]. Most reports believe that surfactants improve the permeability of cell membrane to facilitate the enzyme release [8,35]. Also the unique ability of biosurfactants to enhance biodegradation has gained more and more attention and will undoubtedly lead to its application in pollution control [37].…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Rhamnolipid from Pseudomonas desmolyticum NCIM‐2112 and its role in the degradation of Brown 3REL

Jadhav

Kalme

Tamboli

et al. 2011

J. Basic Microbiol.

View full text Add to dashboard Cite

The biosurfactant produced by Pseudomonas desmolyticum NCIM 2112 (Pd 2112) was confirmed as rhamnolipid based on the formation of dark blue halos around the colonies in CTAB-methylene blue agar plates and the content of rhamnose sugar. The average yield of rhamnolipid was 0.398 g/l/day when grown on hexadecane as sole carbon source. Pd 2112 emulsification potential associated with cell free culture broth was stable for 72 h using various hydrocarbons and vegetable oils. Chemical structure of the biosurfactant was identified as mono-rhamnolipid (Rha-C(6) -C(8) ) using HPTLC, fourier transform infrared spectroscopy, (1) H and (13) C NMR and gas chromatography-mass spectroscopy analysis. Pd 2112 mono-rhamnolipid (1 mg/ml) had increased permeabilization of Bacillus sp VUS NCIM 5342 and increased decolorization rate of textile dye Brown 3REL by 50%. Extracellular activities of lignin peroxidase and veratryl alcohol oxidase, enzymes involved in dye degradation, were significantly increased in the presence of mono-rhamnolipid by 324.52% and 100% respectively. Scanning electron micro-scopy observations revealed that rhamnolipid did not exert any disruptive action on Bacillus cells as compared to Tween 80. The mono-rhamnolipid of Pd 2112 has potential for its application in biodegradation of textile dyes.

show abstract

Section: Discussionmentioning

confidence: 96%

“…It is believed that surfactants alter the cell membranes to facilitate enzyme release [8]. There remains a dearth in availability of reports on the influence of biosurfactants on the enzymes involved in microbial dye degradation.…”

mentioning

confidence: 99%

Rhamnolipid from Pseudomonas desmolyticum NCIM‐2112 and its role in the degradation of Brown 3REL

Jadhav

Kalme

Tamboli

et al. 2011

J. Basic Microbiol.

View full text Add to dashboard Cite

show abstract

“…When adding surfactants to such system, these interactions might have a change, which might overcome the problem of limited bioavailability of substrates in fermentation [25]. First, surfactants improved the permeability of the cell membrane to facilitate laccase release [15,16] and improve phenol degradation. Similar phenomena about various surfactants, such as sodium dodecyl sulfate, Tween-80, diRL, and so on, in favor of other enzyme activities, including CMCase, xylanase, lignin peroxidase, and amylase, have also been reported previously [14,16,17].…”

Section: Effect Of Biosurfactants On Laccase Activitymentioning

confidence: 99%

Effect of Biosurfactants on Laccase Production and Phenol Biodegradation in Solid-State Fermentation

Zhou

Yuan

Zhong

et al. 2010

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The effects of two biosurfactants, tea saponin (TS) and rhamnolipid (RL), on the production of laccase and the degradation of phenol by P. simplicissimum were investigated in solid-state fermentation consisting of rice straw, rice bran, and sawdust. Firstly, the effects of phenol on the fermentation process were studied in the absence of surfactants. Then, a phenol concentration of 3 mg/g in the fermentation was selected for detailed research with the addition of biosurfactants. The results showed that TS and RL at different concentrations had stimulative effects on the enzyme activity of laccase. The highest laccase activities during the fermentation were enhanced by 163.7%, 68.2%, and 23.3% by TS at concentrations of 0.02%, 0.06%, and 0.10%, respectively. As a result of the enhanced laccase activity, the efficiency of phenol degradation was also improved by both biosurfactants. RL caused a significant increase of fungal biomass in the early stage of the fermentation, while TS had an inhibitory effect in the whole process. These results indicated that RL could mitigate the negative effects of phenol on fungal growth and consequently improve laccase production and phenol degradation. TS was potentially applicable to phenol-polluted solid-state fermentation.

show abstract

“…Supplementation of substrate with surfactants has also been explored for increasing enzymes production (Lestan et al, 1993;Zheng and Obbard, 2001;Urek and Pazarlioglu, 2007;Zhang and Cheung, 2011;Usha et al, 2014). They increase the bioavailability of less soluble substrates for the fungus, improve the permeability of cell membrane leading to greater secretion of the extracellular enzyme, enhance enzyme stability and also prevent the denaturation of enzymes during hydrolysis by desorbing them from substrate (Helle et al, 1993;Ahuja et al, 2004;Reddy et al, 1999;Zeng et al, 2006;Liu et al, 2008;Zhou et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of surfactants on lignin modifying enzyme activity of Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation

Singh¹,

Singh²

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Lignin modifying enzyme (LME) activity [laccase, lignin peroxidase (LiP) and manganese peroxidase (MnP)] was studied in two indigenously isolated white rot fungi, Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation (SSF) of wheat bran and saw dust supplemented with various non-ionic polysorbate surfactants (Tweens) and Triton X-100. Both fungal species proved to be highly ligninolytic and promising laccase producers. Their laccase activity increased many folds due to supplementation of substrates with surfactants. Among surfactants, Tween 20 was the best, giving 39180 IU/g laccase in Ganoderma spp. and 37780 IU/g in Lentinus spp. on wheat bran corresponding to 3.8-and 10.6-fold increase over control condition. Triton X-100 was the best surfactant providing 29380 and 19877 IU/g laccase activity in Ganoderma spp. and Lentinus spp. on saw dust amounting to 2.9-and 3.9-fold increase over control condition, respectively. Ganoderma spp. always produced more laccase than Lentinus spp. on supplemented substrates. Between substrates, laccase production on supplementation was always more on wheat bran than saw dust. LiP and MnP activities were very low in comparison with laccase in both fungi under control conditions (1.92 to 4.07 IU/g and 0.98 to 2.07 IU/g, respectively) and upon treatment with surfactants; they did not show an appreciable change, thus, supplementation mediated ratio between laccase to LiP/MnP was larger.

show abstract

Production of an endoglucanase by the shipworm bacterium, Teredinobacter turnirae

Cited by 22 publications

References 22 publications

Rhamnolipid from Pseudomonas desmolyticum NCIM‐2112 and its role in the degradation of Brown 3REL

Rhamnolipid from Pseudomonas desmolyticum NCIM‐2112 and its role in the degradation of Brown 3REL

Effect of Biosurfactants on Laccase Production and Phenol Biodegradation in Solid-State Fermentation

Effect of surfactants on lignin modifying enzyme activity of Ganoderma species KX879638 and Lentinus species KY006984 under solid state fermentation

Contact Info

Product

Resources

About