Production of an active feline interferon in the cocoon of transgenic silkworms using the fibroin H-chain expression system

Kurihara, Hiroyuki; Sezutsu, Hideki; Tamura, Toshiki; Yamada, Katsushige

doi:10.1016/j.bbrc.2007.02.055

Cited by 60 publications

(54 citation statements)

References 22 publications

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“…Although silkworms are not recognized as a major recombinant protein production platform (21)(22)(23), they might be the perfect host for spider silk fiber production because transgenic silkworms can be efficiently produced using piggyBac vectors (24)(25)(26), recombinant protein production can be targeted to the silk gland with tissuespecific promoters (27)(28)(29)(30)(31)(32), and the silk gland is naturally equipped to assemble silk proteins into fibers (33). One caveat is that silkworms produce endogenous silk proteins.…”

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confidence: 99%

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Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties

Teulé

Miao

Sohn

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

239

158

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The development of a spider silk-manufacturing process is of great interest. However, there are serious problems with natural manufacturing through spider farming, and standard recombinant protein production platforms have provided limited progress due to their inability to assemble spider silk proteins into fibers. Thus, we used piggyBac vectors to create transgenic silkworms encoding chimeric silkworm/spider silk proteins. The silk fibers produced by these animals were composite materials that included chimeric silkworm/spider silk proteins integrated in an extremely stable manner. Furthermore, these composite fibers were, on average, tougher than the parental silkworm silk fibers and as tough as native dragline spider silk fibers. These results demonstrate that silkworms can be engineered to manufacture composite silk fibers containing stably integrated spider silk protein sequences, which significantly improve the overall mechanical properties of the parental silkworm silk fibers.biomaterials | biotechnology | transgenic animals

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confidence: 99%

“…1). These included the Bombyx mori fibroin heavy chain (fhc) promoter to target expression of the foreign spider silk protein to the posterior silk gland (29,30) and an fhc enhancer to increase expression levels to facilitate assembly of the foreign silk protein into fibers (31). Our piggyBac vectors also encoded A2S8 14 ( Fig.…”

mentioning

confidence: 99%

Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties

Teulé

Miao

Sohn

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

239

158

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“…Especially, the silk gland of silkworm is a useful tool to produce some valuable recombinant proteins, which can be widely used in the biological, biotechnical and pharmaceutical application fields (Kurihara et al, 2007;Thomas et al, 2002). Recently, Silk-based materials with improved antibacterial properties, mechanical properties, biodegradation and biocompatibility, have been used for applications in bioengineering of bone, skin, cartilage, ligaments, tendons, vascular, neural, ocular, cardiac, and bladder tissues etc (Tatemastu et al, 2012;Zhao et al, 2009).…”

Section: Plasmid Dna Constructionmentioning

confidence: 99%

Expression of the cyan fluorescent protein in fibroin H-chain of transgenic silkworm

Goo¹,

Choi²,

Kim³

et al. 2017

International Journal of Industrial Entomology

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We constructed the fibroin H-chain expression system to produce enhanced cyan fluorescent proteins (ECFP) in transgenic silkworm cocoon. Fluorescent cocoon could be made by fusing ECFP cDNA to the heavy chain gene and injecting it into a silkworm. The ECFP fusion protein, each with N-and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the ECFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworms. The EGFP fluorescence became visible in the ocelli and in the central and peripheral nervous system on the seventh day of embryonic development. A mixture of the donor and helper vector was micro-injected into 1,020 Kumokjam, bivoltin silkworm eggs. We obtained 6 broods. The cocoon was displayed strong blue fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the ECFP fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.

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“…By screening silkworm strains collected by NBRP, we discovered some which are virus-resistant [6]. This production system of targeted proteins has already been commercialized by some business corporation [3,4,10].…”

Section: Utilization As a Protein Production Systemmentioning

confidence: 99%

The Silkworm-An Attractive BioResource Supplied by Japan

et al. 2010

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Abstract:Silkworms have played an important agricultural role in supporting Japan's modernization, and traditionally, Japan has led the world as a repository of silkworm bioresources. The silkworm is a small and highly domesticated insect, which is ideal as a laboratory tool, although it is a bioresource that is relatively infrequently used in experiments at present. In this review, we describe the potential for silkworm resources to contribute to life sciences.

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Production of an active feline interferon in the cocoon of transgenic silkworms using the fibroin H-chain expression system

Cited by 60 publications

References 22 publications

Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties

Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties

Expression of the cyan fluorescent protein in fibroin H-chain of transgenic silkworm

The Silkworm-An Attractive BioResource Supplied by Japan

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