Production of all-female triploid rainbow trout

Lincoln, R.F.; Scott, Alexander P.

doi:10.1016/0044-8486(83)90179-5

Cited by 113 publications

(55 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Asterisks *, ** express significant differences (P < 0.05, P < 0.01) between the groups compared. The model of variance analysis: one-way ing season, polyploidization) on fat content in flesh were also described in other fish species (trout by Lincoln, 1987; sardines by Bandarra et al, 1997). Positive nitrogen balance in the fish organism caused the increase in nitrogenous substances (CP, NP, NMP) in all groups of tench during the period of study (Figure 2).…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Chemical composition of flesh of diploid and triploid population of tench (Tinca tinca, Linnaeus 1758)

Buchtová¹,

Vorlová²,

Svobodová³

et al. 2005

CZECH J ANIM SCI

View full text Add to dashboard Cite

The objective of this study was to determine differences in relevant parameters of the basic chemical composition of flesh (descriptors: dry matter -DM, crude proteins -CP, net proteins -NP and net muscle proteins -NMP, fat -F, ash -A) of communal stock of diploid (2n) and triploid (3n) tench (Tinca tinca L.) of the same origin, in relation to sex (F: female vs. M: male) and age (T 3 : 36 months, after overwintering vs. T 3+ : 42 months, after one summer in the pond). Altogether 137 siblings of tench were studied. The basic chemical composition of flesh of 2n and 3n tench of T 3 age was found to be nearly the same. The only significant difference (P < 0.05) was found in DM content in diploids (5.7% higher DM content in females). Marked changes in the basic chemical composition were related to the increasing age of tench: after one summer in the pond (from T 3 to T 3+ ) the DM content increased significantly in all groups of tench (by 5.4% in 2n females, P < 0.05; by 6.5% in 2n males, 9.9% in 3n females and by 7.4% in 3n males, all at P < 0.01). The content of nitrogenous substances was found to increase significantly (P < 0.01) in flesh of 2n males: CP by 3.2%, NP by 4.2% and NMP by 3.3% and in flesh of 3n males: CP by 7.0%, NP by 6.6% and NMP by 7.3%. Muscles of 3n females contained 107.3% higher fat content (P < 0.05). The proportion of ash decreased in 2n and 3n by 2.5% and 1.7%, respectively (P < 0.05). The flesh quality of T 3+ tench after one summer in the pond was higher if compared with that of T 3 tench after overwintering. Related to the ploidy level, more favourable chemical composition of flesh and more favourable flesh quality were found for triploids of T 3+ age.

show abstract

Section: Discussionmentioning

confidence: 98%

“…Yearly stable colour of flesh of polyploid fish along with lower content of water and higher content of fat was reported by Lincoln (1987), who studied triploid populations of rainbow trout (Oncorhynchus mykiss).…”

mentioning

confidence: 99%

Chemical composition of flesh of diploid and triploid population of tench (Tinca tinca, Linnaeus 1758)

Buchtová¹,

Vorlová²,

Svobodová³

et al. 2005

CZECH J ANIM SCI

View full text Add to dashboard Cite

show abstract

“…Eggs of the "Butley" strain were obtained from a commercial trout farm. Fertilization took place on January 24, 1985, and triploidy was induced in approximately half the eggs by applying to them a heat shock at 28" for 10 mitt, starting 30 min after fertilization (Lincoln and Scott, 1983) and then returning them to water at 10". The eggs and fry were reared in dechlorinated mains water at the Lowestoft laboratory and finally transferred as two separate groups of diploids and triploids to compartments (9.4 m3) of a raceway system (totalling 45 m3) in which mains water was recycled at the rate of 1 pass every 4 hr, with 10% replacement per day.…”

Section: Methodsmentioning

confidence: 99%

“…We used both diploid and triploid fish (Lincoln and Scott, 1983) of each sex because this allowed us to monitor some fish that did not show any gonadal growth, others that showed considerable, but not full, gonadal growth, and others that matured normally during the experiment, yet all were of the same age and genetic stock (Lincoln and Scott, 1984).…”

Section: Accepted August 2 1990mentioning

confidence: 99%

Plasma growth hormone levels during sexual maturation in diploid and triploid rainbow trout (Oncorhynchus mykiss)

Sumpter

Lincoln

Bye

et al. 1991

General and Comparative Endocrinology

Self Cite

View full text Add to dashboard Cite

Plasma growth hormone concentrations were determined in diploid and triploid rainbow trout of both sexes during sexual maturation. Diploid females grow large ovaries, whereas triploid female trout show no ovarian development. The plasma growth hormone concentration in triploid female trout remained low and unchanged throughout the study, whereas it rose slightly, but significantly, in the diploid females that matured, but not in those that remained immature. On the other hand, triploid males do develop testes like their diploid counterparts. In both groups spermiation was accompanied by a steady rise in the plasma growth hormone concentration. The results suggest that the elevated growth hormone concentration in mature male trout was a consequence not of reproduction per se, but of the loss of condition that accompanied spawning. This hypothesis was supported by the results from the females. Apart from a temporary loss in the mature diploids caused by stripping of the eggs, female trout did not lose condition, neither did they show any change in the plasma growth hormone concentration during the period when they ovulated. These results suggest that it was the nutritional insuffrciency accompanying reproduction in male fish that caused the elevation in growth hormone concentration. In fact, a strong negative correlation between the plasma growth hormone concentration and the condition factor of the fish was observed. Q

show abstract

“…L'une d'entre-elles repose sur la création d'animaux triploïdes (possédant trois exemplaires de chaque chromosome au lieu de deux chez les individus normaux diploïdes) qui, selon plusieurs auteurs, montreraient un développement extrêmement réduit de la gonade, au moins chez les femelles (THOR-GAARD et GALL, 1979 ;LINCOLN et SCOTT, 1983). Au cours des trois dernières années, le laboratoire de Physiologie des Poissons de l'INRA a mis au point une méthode simple permettant l'obtention de tels animaux triploïdes chez la truite arc-en-ciel (CHOURROUT, 1980 ;CHOURROUT et QUILLET, 1982).…”

unclassified

Note technique : obtention d'animaux triploïdes chez la truite arc-en-ciel

Chevassus¹,

Quillet²,

Chourrout³

1983

Bull. Fr. Piscic.

View full text Add to dashboard Cite

Bull. Fr. Piscic, 290,[161][162][163][164] -161 -Chez les Salmonidés, l'obtention d'animaux ne présentant pas de maturation sexuelle apparaît intéressante dans plusieurs cas : en élevage intensif, elle permet d'envisager la production d'animaux de grande taille sans se heurter aux difficultés inhérentes à la maturation sexuelle (ralentissement de la croissance, mortalités plus élevées, diminution de la qualité de la chair) ; dans le cas de peuplements extensifs, elle autorise le déversement d'animaux dont on souhaite éviter la prolifération éventuelle ou le croisement avec les individus résidents.Différentes méthodes ont été proposées pour aboutir à un tel résultat (CHEVAS-SUS et al, 1979 b ;BILLARD, 1983). L'une d'entre-elles repose sur la création d'animaux triploïdes (possédant trois exemplaires de chaque chromosome au lieu de deux chez les individus normaux diploïdes) qui, selon plusieurs auteurs, montreraient un développement extrêmement réduit de la gonade, au moins chez les femelles (THOR-GAARD et GALL, 1979 ;LINCOLN et SCOTT, 1983). Au cours des trois dernières années, le laboratoire de Physiologie des Poissons de l'INRA a mis au point une méthode simple permettant l'obtention de tels animaux triploïdes chez la truite arc-en-ciel (CHOURROUT, 1980 ;CHOURROUT et QUILLET, 1982). Une telle méthode, combinée aux techniques de production de populations" monosexes femelles " (CHEVASSUS et al., 1979 a) pourrait donc fournir des populations contenant une très forte proportion d'animaux stériles. Elle est basée sur l'application d'un choc chaud peu après la fécondation qui, en inhibant la dernière étape de la méiose femelle, permet d'obtenir des individus présentant au niveau de leur noyau un stock génétique supplémentaire issu de leur mère, qui vient s'ajouter aux deux stocks génétiques d'origine maternelle et paternelle (apportés respectivement par l'ovule et le spermatozoïde) que possèdent les individus normaux diploïdes. Nous présentons dans cette note technique :-le protocole précis permettant l'obtention d'animaux triploïdes, -quelques indications sur les caractéristiques zootechniques de ces animaux, basées sur nos observations des deux dernières années. I. PROTOCOLELa méthode consiste à tremper les oeufs dans un bain-marie à 26° ± 0,5° C pendant 20 mn peu après la fécondation. On procédera de la manière suivante : 1) FécondationNous distinguerons le cas où l'on utilise la méthode sèche traditionnelle et celui où l'on utilise le dilueur 532 pour l'insémination artificielle (BILLARD, 1977). Les quantités de sperme mentionnées sont celles utilisées traditionnellement en pisciculture. L'utilisation du dilueur permet de réduire éventuellement ces quantités (BILLARD, 1975 RÉSUMÉNous présentons dans cette note le protocole d'obtention d'animaux triploïdes par choc thermique chez la truite arc-en-ciel, ainsi que quelques indications sur l'intérêt et les caractéristiques zootechniques de ces animaux. SUMMARYA method to obtain triploid animals in rainbow trout by heat shock is described. Some indications on the pr...

show abstract

Production of all-female triploid rainbow trout

Cited by 113 publications

References 10 publications

Chemical composition of flesh of diploid and triploid population of tench (Tinca tinca, Linnaeus 1758)

Chemical composition of flesh of diploid and triploid population of tench (Tinca tinca, Linnaeus 1758)

Plasma growth hormone levels during sexual maturation in diploid and triploid rainbow trout (Oncorhynchus mykiss)

Note technique : obtention d'animaux triploïdes chez la truite arc-en-ciel

Contact Info

Product

Resources

About