Production of alkaline protease by a genetically engineered Aspergillus oryzae U1521.

Samarntarn, Warin; Cheevadhanarak, S.; Tanticharoen, Morakot

doi:10.2323/jgam.45.99

Cited by 18 publications

(10 citation statements)

References 16 publications

(8 reference statements)

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“…under SSF in green gram husk medium supplied with xylose and maltose. Samarntarn et al (1999) reported that protease production by Aspergillus oryzae U152 was high in the presence of supplementary carbohydrate carbon sources, especially lactose. Despite an enhanced protease production under SSF by S. proteamaculans AP-CMST in medium with added surfactants, negative results over the control medium (148.15 U/g) were also noted in certain cases.…”

Section: Resultsmentioning

confidence: 99%

Solid-state protease production using anchovy waste meal by moderate halophile Serratia proteamaculans AP-CMST isolated from fish intestine

et al. 2011

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The possibility of utilising anchovy waste meal as a substrate for protease production by the fish gut isolate Serratia proteamaculans AP-CMST was assessed through solid-state fermentation. A time course for protease production revealed 72 h to be the optimum duration for higher production (146.24 U/g). The most suitable pH, temperature and moisture level observed for higher protease production were pH 7 (123.5 U/g), 30°C (97.22 U/g) and 75% (126.7 U/ g), respectively. Protease production by S. proteamaculans AP-CMST was high in medium with added xylose (198.21 U/g), peptone (118.42 U/g), Triton X-100 (152.56 U/g) and manganese sulphate (178.33 U/g) when compared to other tested medium components. The halotolerancy of S. proteamaculans AP-CMST for protease production was 4% sodium chloride (155.65 U/g). Enzyme recovery from fermented anchovy waste meal was greatest (130.52 U/g) when 10% ethyl acetate was used as the extractant, and the optimum time range for extraction was 90-120 min.

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Section: Resultsmentioning

confidence: 99%

Solid-state protease production using anchovy waste meal by moderate halophile Serratia proteamaculans AP-CMST isolated from fish intestine

et al. 2011

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“…Highest proteolytic activity was seen after 120 h at 1.5% casein concentration (Table 3) and the amount of liberated tyrosine reached 40.3 mg/g substrate. It was reported that casein induced protease production at low concentrations and served as nitrogen metabolite repressor at high concentration in many types of yeast[7]. …”

Section: Resultsmentioning

confidence: 99%

Optimization of cultural conditions for protease production by a fungal species

Kamath

Rao

et al. 2010

Indian J Pharm Sci

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Studies were carried out on a paddy soil fungal isolate identified to be a strain of Aspergillus niger from Manipal. The parameters that largely impact enzyme production viz., fermentation time, impeller speed, pH, temperature and nutrient supplements were studied. Optimization of production parameters for production of protease was done by the single-parameter mode. Casein served as substrate and proteolytic activity was estimated using Folin-Ciocalteau method at 660 nm. A maximum yield of 71.3 mg tyrosine/g casein substrate was produced in 96 h on a soluble starch medium at pH 4 in shake flask experiments. Production was carried out on a 3-liter fermenter and 40.7 mg of tyrosine was liberated/g of substrate. The enzyme was extracted with 50% ammonium sulfate and sodium dodecyl sulfate-Polyacrylamide gel electrophoresis showed two bands having mw 45.7 kDa and 38.5 kDa, respectively. The enzyme activity was found to be 147.84 U/ml.

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“…(Prakasham et al, 2006). Samarntarn et al (1999) reported that protease production was high in the presence of supplementary carbohydrate carbon sources, especially lactose for microbes.…”

Section: Effect Of Carbon Source On Protease Activitymentioning

confidence: 99%

Optimization of alkaline protease production from Bacillus subtilis NS isolated from sea water

Nisha¹,

Divakaran²

2014

Afr. J. Biotechnol.

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The protease producing bacterial strain Bacillus subtilis was isolated from sea water and identified by 16S rRNA sequencing. The strain named as B. subtilis NS. Optimization of the strain revealed that the most suitable nitrogen source to enhance protease production was beef extract. Among various carbon sources tested, maximum production of protease was registered in medium with added glucose. The effect of metals ions indicated that maximum protease production was observed in medium supplemented with magnesium chloride (MgCl). Investigating the effect of sodium chloride (NaCl) concentration on protease production revealed that 7% yielded higher protease production. The most suitable pH and temperature for maximum protease production revealed that pH 9 and a temperature of 40°C gave optimal protease production.

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Production of alkaline protease by a genetically engineered Aspergillus oryzae U1521.

Cited by 18 publications

References 16 publications

Solid-state protease production using anchovy waste meal by moderate halophile Serratia proteamaculans AP-CMST isolated from fish intestine

Solid-state protease production using anchovy waste meal by moderate halophile Serratia proteamaculans AP-CMST isolated from fish intestine

Optimization of cultural conditions for protease production by a fungal species

Optimization of alkaline protease production from Bacillus subtilis NS isolated from sea water

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