Production of Alkaline Lipase by Corynebacterium paurometabolum, MTCC 6841 Isolated from Lake Naukuchiatal, Uttaranchal State, India

Joshi, Gopal; Kumar, Sarvesh; Tripathi, Bhumi Nath; Sharma, Vinay

doi:10.1007/s00284-005-0224-6

Cited by 26 publications

(20 citation statements)

References 17 publications

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“…Soyabean, coconut and amla oil supported the growth of fungus (30). The highest lipase activity (12.1 U/ml) was observed with coconut oil (1% v/v) as a substrate (21).…”

Section: Discussionmentioning

confidence: 96%

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Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

Shreya

Sharma

et al. 2018

Plant Sci. Today

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The present study aimed to optimize culture conditions for optimal growth and production of extracellular lipase. Lipolytic fungal strain named as S3St2 previously isolated from a petrol pump soil sample of Newai Town was used for optimization study. Among the tested carbohydrate carbon sources, polysaccharide-starch exhibited maximum lipase production (21.25±0.70 IU/ml/min) with highest specific activity (1.47±0.06 U/mg). Lipase activity and specific activity were higher with mustard oil 1 % (v/v) among all lipidic carbon sources. Among inorganic nitrogen source, potassium nitrate was found best inducer of lipase activity, malt extract supported the fungus growth (dry weight of cell pellets was 0.467 g) and exhibited maximum lipase activity among all organic nitrogen sources. Lipase activity was optimum at pH 8.0, indicates alkalophillic nature of production media supports the growth of fungus. Higher lipase activity (27.92±0.87 IU/ml/min) was detected at 28ºC. The incubation time of 5 days was found optimum for maximum lipase production (31.51±0.21 IU/ml/min). Keywords

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“…Soyabean, coconut and amla oil supported the growth of fungus (30). The highest lipase activity (12.1 U/ml) was observed with coconut oil (1% v/v) as a substrate (21).…”

Section: Discussionmentioning

confidence: 96%

“…One flask was kept as control containing olive oil as original carbon source (20). Lipase activity and protein contents (21,22) were determined after 5 days of incubation (23). Flaks were inoculated with one ml of spore suspension from slant culture.…”

Section: Optimization Of Lipidic Carbon Source For Lipase Productionmentioning

confidence: 99%

Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

Shreya

Sharma

et al. 2018

Plant Sci. Today

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“…Activity of lipase was measured by the protocol as described previously by Joshi et al (2006). One unit (U) of enzyme activity was equivalent to how many micromoles (µM) of p-nitrophenol liberated by hydrolysis of ester linkage of pNPP by one mL of soluble enzyme at 35 °C in one minute of reaction.…”

Section: Lipase Assaymentioning

confidence: 99%

Enhancement of Extracellular Lipase Production by Strain Improvement of Fungus Aspergillus niger LPF-5

Sharma¹,

Sharma²,

Saxena³

2016

IJSRES

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Abstract. Twelve fungal isolates belonging to different genera were quantitatively screened for extracellular lipase production in SmF (Submerged fermentation). Isolate LPF-5 demonstrated higher lipase activity and it was identified as Aspergillus niger based on morphology of its Petri plate culture, microscopic study of its slide culture and with the help of relevant literatures. Further an effort was made to increase the production of extracellular lipase by subjecting the most potent lipolytic fungal strain A. niger LPF-5 to the strain improvement by induced mutagenesis using UV radiations and nitrous acid. Among the all tested mutagens, nitrous acid was found to be the best mutagen (incubation time of 15 minutes) for inducing the favorable mutation in fungal strain A. niger LPF-5 which enhanced the lipase activity up to 30% (105.19 ± 0.91 U mL -1 min -1) when compared to lipase activity (81.00 ± 0.30 U mL -1 min -1 ) of wild strain while the lipase activity of the best UV mutant (A. niger UV3), obtained after an incubation of 6 minutes was 94.30 ± 0.54 U mL -1 min -1 , which was 16.41% higher as compared to wild strain of A. niger. These results indicate that UV light and nitrous acid both were effective mutagens but nitrous acid was more potent mutagen than UV light.

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“…Lipase activity was assayed by Para-nitro Phenyl Palmatate Assay which is further modified method of Winkler and Stuckmann (1979). The fermented broth was centrifuged at 10,000 rpm at 4 0 C for 20 minutes (Joshi et al, 2006).The supernatant obtained was used as a crude enzyme for lipase assay (Qamsari et al, 2011). This crude enzyme (0.75 ml) was mixed with 0.5mM of 4-nitrophenyl palmitate substrate prepared in isopropyl alcohol and 1.95 ml of 50mM Phosphate Buffer (pH7.2) incubated at 30°C for 30 minutes.…”

Section: Quantitative Lipase Assaymentioning

confidence: 99%

Bioremediation of temple waste (nirmalya) by vermicomposting in a metropolitan city like Mumbai

2016

Int. J. Curr. Res. Biol. Med.

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In India, million tons of temple waste (nirmalya) is produced every day. The waste collected from temple mainly consists of flowers, leaves, fruits, honey, coconuts, camphor, jaggery, milk etc. which is released in the water bodies or dumped at the available land spaces, thereby leading to severe environmental pollution and health hazards. Bioremediation of nirmalya can be carried out by vermicomposting. Vermicomposting is an eco-friendly process of efficiently converting organic waste into compost with the help of soil microorganism and earthworms. In our work Nirmalya was taken from a temple in South Mumbai, which was pre-composted at 30 o C and used as a substrate for vermicomposting by earthworm species Eisenia foetida for 90 days. The chemical analysis of the vermicompost showed its pH (7.2), the organic carbon content (8.57%), N (0.49%), total P (0.5%), K (0.16%), C: N ratio (17.489) and also contained sufficient concentration of microelements like zinc, manganese, iron and copper. The total bacterial count of vermiwash was found to be 3×10 9 cfu/ml. The bacteria which were isolated from vermiwash showed various enzyme activities like protease, cellulase, phosphatase, amylase, gelatinase and lipase. The presence of nitrogen fixing bacteria like Azotobacter and Rhizobium from vermiwash was also demonstrated. The vermicompost obtained was checked for its effect on the growth of the test plants like Tagetes erecta and Solanum melongena using pot culture studies. One of the bacterial isolates was identified as Bacillus amyloliquefaciens HY10 by morphological, cultural, biochemical and 16s rRNA sequence analysis which showed protease (32.53units/ml) and lipase activity (3.177units/ml).

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Production of Alkaline Lipase by Corynebacterium paurometabolum, MTCC 6841 Isolated from Lake Naukuchiatal, Uttaranchal State, India

Cited by 26 publications

References 17 publications

Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

Enhancement of Extracellular Lipase Production by Strain Improvement of Fungus Aspergillus niger LPF-5

Bioremediation of temple waste (nirmalya) by vermicomposting in a metropolitan city like Mumbai

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