Production of Aldonic Acids from Monosaccharides by Washed Cells of Burkholderia cepacia and their Calcium Binding Capability

洋, 村上; 高明, 桐生; 太郎, 木曽; 博文, 中野

doi:10.5458/jag.53.277

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…17) Similarly, effective conversions of D galactose, D xylose and L arabinose to corresponding calcium aldonates were also attained by the fungal enzyme. These calcium aldonates including lactobionate were highly soluble in water.…”

Section: Enzymatic Production Of Other Aldonic Acidsmentioning

confidence: 97%

“…We screened microorganisms that not only possessed lactose oxidizing activity but also lacked β galactosidase activity to decompose lactose and LacA; both seemed to be requisite for achieving high yields of LacA. 14 19) A strain of Burkholderia cepacia that exhibited the highest conversion ability among the isolates and had a membrane bound enzyme had been applied to fermentation 14,15) and microbial conversion processes 16,17) in our earlier studies. Paraconiothrium sp.…”

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confidence: 99%

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Production of Calcium Lactobionate by a Lactose-oxidizing Enzyme from <i>Paraconiothyrium</i> sp. KD-3

et al. 2008

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A lactose-oxidizing enzyme was obtained from culture supernatant of a fungal strain of Paraconiothyrium sp. KD-3. The enzyme was a flavoprotein with a molecular mass of 54 kDa. The purified enzyme oxidized various monosaccharides and oligosaccharides such as D-glucose, D-galactose, D-xylose, cellooligosaccharides and maltooligosaccharides in addition to lactose, using molecular oxygen as a good electron acceptor, to accumulate the corresponding aldonic acids and hydrogen peroxide. The Paraconiothyrium enzyme was suitable for the production of calcium lactobionate compared with a commercial hexose oxidase owing to the stability during the conversion. The enzyme converted 10 20% (w v) lactose completely to calcium lactobionate in a 500-mL reactor under aeration, agitation and pH regulation at 5.5. The neutralization was successfully performed by the addition of 10% (w w) slurry of calcium carbonate, while neutralization with 25% (w v) sodium hydroxide solution inactivated the enzyme gradually. The supplement of catalase to the mixture promoted the conversion by degrading hydrogen peroxide. The immobilized enzyme, which was prepared by the adsorption to a cation exchange resin followed by the condensation with carbodiimide, oxidized 18.5% (w v) lactose to produce calcium lactobionate in a batch reaction. Calcium lactobionate and aldonates derived from D galactose, D-xylose and L-arabinose showed high aqueous solubility and low calcium binding capability.

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Section: Enzymatic Production Of Other Aldonic Acidsmentioning

confidence: 97%

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confidence: 99%

Production of Calcium Lactobionate by a Lactose-oxidizing Enzyme from <i>Paraconiothyrium</i> sp. KD-3

et al. 2008

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“…E. ( Miyamoto et al ,2000 ). 2.7.4.4. lactose ,10; corn steep liquor, 3ml; yeast extract, 0.02 and CaCO 3 , 1 (Murakami et al,2006b).…”

Section: 73inoculum Sizementioning

confidence: 99%

“…The maximal yields (87and 90%) after 7 days fermentation were obtained at pH 5.0 to 6.0. The pH of the flask culture could be maintained within the range by the addition of calcium carbonate as a potential-alkali (Miyamoto et al,2000 andMurakami et al,2006b).…”

Section: Ph Valuementioning

confidence: 99%

Conversion of Lactose to Calcium Lactobionate by Some Bacterial Species

El-Hadi

El-Minofi

El-Beih

et al. 2010

Egyptian Journal of Agricultural Sciences

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Bacterial conversion of lactose to calcium lactobionate was carried out by twelve strains of bacteria. The strain Bacillus spp. E showed high lactose oxidizing activities, when cultivated at 30˚C for 7 days in a medium ( pH 6) containing (g/100ml): lactose ,5; KH 2 PO 4 ,0.06 ; MgSO 4 .7H 2 O, 0.025; corn steep liquor ,0.75 ml ; 1 drop of soybean oil ; 1.5ml of 20% urea solution and CaCO 3 ,2.5 added during inoculation. The oxidizing activity was the highest where a relatively high lactose conversion (82%) was obtained at a lactose concentration of 50g/l . The activity remained almost stable between pH 5 and 6 and high conversion efficiency ( about 90%) was achieved at pH 0.6.

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