Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor

Cui, Xi-Hua; Murthy, Hosakatte Niranjana; Jin, You-Xun; Yim, Yong‐Hyeon; Kim, Ji Yeong; Paek, Kee-Yoeup

doi:10.1016/j.biortech.2011.08.044

Cited by 67 publications

(29 citation statements)

References 37 publications

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“…Adventitious roots (2.0 kg) of H. perforatum was kindly supplied from Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Korea in 2010. 35 A voucher specimen (CNU11101) was deposited at the Herbarium of College Table 1. General Acid Hydrolysis.…”

Section: Methodsmentioning

confidence: 99%

NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

Li¹,

Ding²,

Quang³

et al. 2013

Bulletin of the Korean Chemical Society

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A new compound, perforaphenonoside A (1), along with 11 known compounds (2-12) were isolated from a methanol extract of adventitious roots of Hypericum perforatum. Their chemical structures were elucidated using chemical and physical methods as well as comparison of NMR and mass spectral data with previously reported data. Their inhibition of NF-κB and activation of PPAR was measured in HepG2 cells using a luciferase reporter system. Among the compounds 3, 6, 7 and 12 inhibited NF-κB activation stimulated by TNFα in a dose-dependent manner, with IC 50 values ranging from 0.85 to 8.10 μM. Moreover, compounds 1-3, 7, 11 and 12 activated the transcriptional activity of PPARs in a dose-dependent manner, with EC 50 values ranging from 7.3 to 58.7 μM. The transactivational effects of compounds 1-3, 7, 11 and 12 were evaluated on three individual PPAR subtypes. Among them, compound 2 activated PPARα transcriptional activity, with 153.97% stimulation at 10 μM, while compounds 1, 2 and 11 exhibited transcriptional activity of PPARγ, with stimulation from 124.76% to 126.91% at 10 μM.

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Section: Methodsmentioning

confidence: 99%

NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

Li¹,

Ding²,

Quang³

et al. 2013

Bulletin of the Korean Chemical Society

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“…The establishment of calli and cell suspension cultures of H. perforatum has also been reported (Dias et al 1998, 2001, Bais et al 2002. In order to increase the production of secondary metabolites of H. perforatum large-scale in vitro production in bioreactors have been reported by few studies (Zobayed and Saxena 2003;Zobayed et al 2004;Cui et al 2011). Nevertheless, low yields of secondary metabolites have been obtained in this kind of cultures (Kirakosyan et al 2004).…”

Section: Biotechnology In Hypericum Essential Oil Improvementmentioning

confidence: 99%

Hypericum sp.: essential oil composition and biological activities

2012

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Phytochemical composition of Hypericum genus has been investigated for many years. In the recent past, studies on the essential oils (EO) of this genus have been progressing and many of them have reported interesting biological activities. Variations in the EO composition of Hypericum species influenced by seasonal variation, geographic distribution, phenological cycle and type of the organ in which EO are produced and/or accumulated have also been reported. Although many reviews attributed to the characterization as well as biological activities of H. perforatum crude extracts have been published, no review has been published on the EO composition and biological activities of Hypericum species until recently (Crockett in Nat Prod Commun 5(9): 1493-1506, 2010 Bertoli et al. in Global Sci Books 5:29-47, 2011). In this article, we summarize and update information regarding the composition and biological activities of Hypericum species EO. Based on experimental work carried out in our laboratory we also mention possible biotechnology approaches envisaging EO improvement of some species of the genus.

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“…In the case of the 3-l BTBB culture of Hypericum perforatum adventitious roots, the biomass reached its peak (15.07 g l −1 DW) after 6 weeks of cultivation [27]. Wang and Qi [4] studied the influence of mist intervals on growth and second metabolite production of P. heterophylla adventitious roots in a siphon-mist bioreactor.…”

Section: Fed-batch Cultivation Of Adventitious Roots In the Bioreactormentioning

confidence: 99%

HPLC–ESI–MSn Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla

Wang

et al. 2015

Appl Biochem Biotechnol

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A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots. Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis. The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %. Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR). The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group. PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group. The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla. As a result, AR possessed a similar immunoregulation function as NR.

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Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor

Cited by 67 publications

References 37 publications

NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

NF-κB Inhibition and PPAR Activation by Phenolic Compounds from Hypericum perforatum L. Adventitious Root

Hypericum sp.: essential oil composition and biological activities

HPLC–ESI–MSn Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla

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