Production of Abiotic Stress Tolerant Fertile Transgenic Plants using Androgenesis and Genetic Transformation Methods in Cereal Crops

Islam, S. M. Shahinul; Tuteja, Narendra

doi:10.1007/978-1-4614-4633-0_9

Cited by 4 publications

(5 citation statements)

References 99 publications

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“…Androgenesis, like other haploid inducing techniques, is influenced by several biotic and abiotic factors. The genotype, physiological state, and growth conditions of donor plants; stage of pollen development; pretreatment of flower buds or anthers; in vitro culture medium composition; and physical factors during tissue culture together with their interactions significantly affect the response of anthers in in vitro culture [ 15 , 16 ]. The application of suitable physiochemical factors promotes a stress response that arrests microspores or young pollen grains in their gametophytic pathway.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Zinc, Copper, and Silver Ions on Oat (Avena sativa L.) Androgenesis

Warchoł

Juzoń

Dziurka

et al. 2021

Plants

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Oat (Avena sativa L.) cultivars ‘Bingo’ and ‘Chwat’ were used to compare the embryogenesis competence of another culture. Despite the embryo-like structures obtained from both tested cultivars, only ‘Chwat’ produced green plantlets, which confirmed the cultivar dependency. ‘Chwat’ produced the highest number of embryo-like structures and green plantlets (0.7/100 anthers and 0.1/100 anthers, respectively). The embryo-like structure formation also depended on cold pretreatment combined with Cu2+, Zn2+, or Ag+ ion supplementation, which was applied during the tiller pretreatment or added to the induction media. The highest number of embryo-like structures (2.1/100 anthers) were observed on anthers derived from the tillers kept in a 50% Hoagland medium with the addition of 10 µM of CuSO4. In turn, the induction media supplemented with the ions Cu2+, Zn2+, or Ag+ increased neither the number of embryo-like structures nor the green plantlet production compared to the control conditions. However, such ion applications turned out to be most effective when the induction medium was enriched with 25 µM of AgNO3 and left to obtain the highest number of embryo-like structures and green plantlets (0.8/100 anthers and 0.2/100 anthers, respectively). Therefore, more attention should be paid to the possibilities of adjusting the media nutrient composition, as this may be the only way to significantly increase the efficiency of this method.

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Section: Introductionmentioning

confidence: 99%

The Effect of Zinc, Copper, and Silver Ions on Oat (Avena sativa L.) Androgenesis

Warchoł

Juzoń

Dziurka

et al. 2021

Plants

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“…1 In spite of many abiotic stresses are played negative role on rice and other crop production. [2][3][4][5] Drought and salinity are the major abiotic stresses that adversely affect the overall metabolic activities and cause plant demise. 6,7 Certain crops reduced their production capabilities in high saline conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

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D number of viable callus; ICn D number of inoculated callus.Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL ¡1) and Kin (1.0 mgL ¡1). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL ¡1 ) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL ¡1 NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL ¡1 salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.

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“…In vitro production of doubled haploid (DH) plants through androgenesis induction is a promising and convenient alternative to traditionally used techniques for rapid production of fully homozygous plants for breeding programs, marker identification, and gene mapping. A significant advantage is that the system not only speeds up the process of obtaining homozygosity but also increases selection efficiency (Islam and Tuteja 2012). Androgenesis is defined as a developmental pathway, alternative to zygotic embryogenesis, driven by a shift of the normal gametophytic development of microspore into the sporophytic development in which embryos and plants are inherited with the genetic traits from the male donor plant.…”

Section: Introductionmentioning

confidence: 99%

“…Androgenesis, like other haploid-inducing techniques, is influenced by several endogenous and exogenous factors. Genotype, physiological state, and growth conditions of donor plants, stage of male gametes, pretreatment of flower buds or anthers, in vitro culture medium composition, and physical factors during tissue culture together with their interactions are the main factors determining the androgenetic response in in vitro culture (Islam and Tuteja 2012;Murovec and Bohanec 2012;Ferrie et al 2014). The application of suitable physiochemical factors promotes stress response that arrests microspores or young pollen grains in their gametophytic pathway.…”

Section: Introductionmentioning

confidence: 99%

Factors inducing regeneration response in oat (Avena sativa L.) anther culture

Warchoł

Czyczyło-Mysza

Marcińska

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

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The efficiency of embryogenesis of anther culture was compared using four cultivars of oat (Avena sativa L.): 'Akt', 'Bingo', 'Bajka', and 'Chwat'. Despite the high resistance of oat to the process of androgenesis, all tested cultivars produced embryo-like structures and only two of them, 'Akt' and 'Chwat', produced fertile doubled haploid plants. A strong cultivar dependency was observed during induction of androgenesis. Further, cold pretreatment together with high temperature shock enhanced the efficiency of this technique. The highest number of embryo-like structures and haploid plants was obtained from cv. 'Chwat' (3.6% and 0.8%, respectively). Embryo-like structure formation also depended on the distance from the base of the flag leaf to the penultimate leaf of the panicle. Most of them were observed on anthers harvested from panicles of which the distance from the base of the flag leaf to the penultimate leaf was less than 4 cm. The presence of the induction medium supplemented with different plant growth regulators was essential for the induction of embryo-like structures but did not increase the production of haploid plants and doubled haploid lines. The highest number of embryo-like structures and plants was obtained on W14 medium with the addition of 2.0 mg/dm 3 2,4-dichlorophenoxyacetic acid and 0.5 mg/dm 3 kinetin (2.7%). The low haploid plant regeneration rate (from 0.03 to 0.05%) still limits the practical application of anther culture for the production of doubled haploid lines in oat.

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Production of Abiotic Stress Tolerant Fertile Transgenic Plants using Androgenesis and Genetic Transformation Methods in Cereal Crops

Cited by 4 publications

References 99 publications

The Effect of Zinc, Copper, and Silver Ions on Oat (Avena sativa L.) Androgenesis

The Effect of Zinc, Copper, and Silver Ions on Oat (Avena sativa L.) Androgenesis

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Factors inducing regeneration response in oat (Avena sativa L.) anther culture

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