Production of a value-added hydroxy fatty acid, 7,10-dihydroxy-8(E)-octadecenoic acid, from high oleic safflower Oil by Pseudomonas aeruginosa PR3

Microbial Conversion of Vegetable Oil to Hydroxy Fatty Acid and Its Application to Bio-Based Polyurethane Synthesis

“…The standard growth medium consisted of 4 g/L glucose, 1 g/L yeast extract, 4 g/L K 2 HPO 4 , 1 g/L (NH 4 ) 2 HPO 4 , 0.1 g/L MgSO 4 , 0.056 g/L FeSO 4 , and 0.01 g/L MnSO 4 . The medium was adjusted to pH 8.0 by dilute phosphoric acid [19]. The strain was grown aerobically in a 500-mL flask containing 100 mL of the standard medium at 28 °C with shaking at 200 rpm.…”

“…Since HFAs were originally found in limited amounts in the plant system, recent studies have focused on microbial conversion processes of various unsaturated fatty acids to HFAs. Pseudomonas aeruginosa PR3 is a known strain with the ability to convert many unsaturated fatty acids to mono-, di-, and tri-hydroxy fatty acids [15,16,17,18,19,20]. Among fatty acid substrates, oleic acid was efficiently used by strain PR3 to produce 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) [21,22].…”

Knockout of secondary alcohol dehydrogenase in Nocardia cholesterolicum NRRL 5767 by CRISPR/Cas9 genome editing technology

“…Hydroxy fatty acids (HFAs) have potential industrial applications as lubricants, waxes, resins, nylons, plastics, cosmetics, additives in coating and paintings, flavors, antimicrobial agent, or precursors for lactones and dicarboxylic acids [1][2][3][4][5]. Unsaturated fatty acids in plant oils can be converted to HFAs chemically or enzymatically [6][7][8][9][10].…”

Identification of molecular species of polyol oils produced from soybean oil by Pseudomonas aeruginosa E03-12 NRRL B-59991

“…Our previous research established that microbial systems can convert FA to ricinoleic acid-type oxygenated FA, including many bioactive FA such as monohydroxy-, dihydroxy-and trihydroxy-unsaturated FA, tetrahydrofuranyl unsaturated FA, and diepoxy bicyclic unsaturated FA (Hou, 1994(Hou, , 1995(Hou, , 1997Hou et al, 1998Hou et al, , 2001Gardner et al 2000;Iwasaki et al, 2002;Hosokawa et al, 2003aHosokawa et al, , 2003bHosokawa et al, , 2003cHou and Hosokawa, 2005;Chang et al, 2007;Su et al, 2011;Bae et al, 2010). However, the biobased polymer industry requires acylglycerol (soybean oil) polyols and not FA polyols.…”