Production of a robust nanobiocatalyst for municipal wastewater treatment

Hommes, Gregor; Gasser, Christoph A.; Howald, Chaim B.C.; Goers, Roland; Schlößer, Dietmar; Shahgaldian, Patrick; Corvini, Philippe F.-X.

doi:10.1016/j.biortech.2011.11.129

Cited by 45 publications

(49 citation statements)

References 33 publications

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“…laccases, such as 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) or dimethoxyphenol. Regarding bisphenol A (BPA), which was the model pollutant addressed in the current research, there are several studies concluding that the immobilized laccases showed significantly lower rates than the free enzyme, probably due to diffusional problems (Hommes et al 2012, Pang et al 2015. This same behavior was also observed in a parallel study (unpublished data) of our group.…”

Section: Introductionsupporting

confidence: 78%

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Arca-Ramos

Eibes

Feijoo

et al. 2015

Appl Microbiol Biotechnol

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In this study, the removal of bisphenol A (BPA) by laccase in a continuous enzymatic membrane reactor (EMR) was investigated. The effects of key parameters, namely, type of laccase, pH, and enzyme activity, were initially evaluated. Once optimal conditions were determined, the continuous removal of the pollutant in an EMR was assessed in synthetic and real biologically treated wastewaters. The reactor configuration consisted of a stirred tank reactor coupled to a ceramic membrane, which prevented the sorption of the pollutant and allowed the recovery and recycling of laccase. Nearly complete removal of BPA was attained under both operation regimes with removal yields above 94.5 %. In experiments with real wastewater, the removal of BPA remained high while the presence of colloids and certain ions and the formation of precipitates on the membrane potentially affected enzyme stability and made necessary the periodic addition of laccase. Polymerization and degradation were observed as probable mechanisms of BPA transformation by laccase.

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Section: Introductionsupporting

confidence: 78%

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Arca-Ramos

Eibes

Feijoo

et al. 2015

Appl Microbiol Biotechnol

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“…Crosslinking via GLU is a complex reaction affected by various factors including amine content of carrier, enzyme type and concentration, time of reaction, pH, among others [30]; thus, appropriate reaction conditions, including GLU concentration, have to be determined for each enzyme-carrier pair and are, in most cases, hardly comparable and transferable. Hommes et al used a similar protocol for laccase immobilization on aminated fumed silica and showed that 1 mmol GLU mg À1 silica ($10 mM) was already sufficient to maximize the immobilization yield and enzyme load [31].…”

Section: Results and Discussion Covalent Immobilization Of Laccases Omentioning

confidence: 99%

Design-of-experiment strategy for the formulation of laccase biocatalysts and their application to degrade bisphenol A

et al. 2012

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“…Measurements using the Clark electrode were performed as described elsewhere. 4 The signal was recorded for 5 min. Data were fitted using the Oxygraph plus software provided by the manufacturer.…”

Section: Analytical Chemistrymentioning

confidence: 99%

Determination of Oxidoreductase Activity Using a High-Throughput Microplate Respiratory Measurement

et al. 2012

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High-throughput multiparallel activity profiling for oxygen consuming cell layers has been recently developed for extracellular flux analysis. This technology has great potential for determining the enzymatic activity of oxidoreductases (i.e., laccase) both in vivo and in vitro, which is usually measured using photometrical tests monitoring the colored oxidation products. Improvements in terms of sample throughput, comparability, and gain of information (i.e., stoichiometry, electron transfer rate) can be achieved by means of a multiwell plate-based fluorimetric oxygen sensor. In the present study, various laccases have been applied to develop protocols that allow the multiparallel measurement of O(2)-consumption by enzymatic reactions. The developed and validated method enables the comparative quantitation of laccase characteristics (i.e., profiles of activity at various pH values) and minimizes the time it usually takes to collect respiratory data of oxygen-consuming enzymes. Furthermore, the possibility to assess differences between single and multisubstrate kinetics of laccases has been demonstrated.

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Production of a robust nanobiocatalyst for municipal wastewater treatment

Cited by 45 publications

References 33 publications

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Potentiality of a ceramic membrane reactor for the laccase-catalyzed removal of bisphenol A from secondary effluents

Design-of-experiment strategy for the formulation of laccase biocatalysts and their application to degrade bisphenol A

Determination of Oxidoreductase Activity Using a High-Throughput Microplate Respiratory Measurement

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