Production of a Recombinant CagA Protein for the Detection of Helicobacter pylori CagA Antibodies

Akgüç, Miray; Karataylı, Ersin; Çelik, Esra Uzer; Koyuncu, Duygu; Çelik, İnci; Karataylı, Senem Ceren; Özden, Ali; Bozdayı, A. Mithat

doi:10.5578/mb.7642

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Vaccination has been established to be a promising way to alleviate severe disease outcomes due to Helicobacter pylori infection. With the advances of genetic modification techniques, the recombinant CagA, VacA and NapA antigens production for resisting Helicobacter pylori infection have been produced in animal systems [ 45 , 46 ] and E. coli [ 47 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

Transient recombinant expression of highly immunogenic CagA, VacA and NapA in Nicotiana benthamiana

Barzigar¹,

Haraprasad

Kumar³

et al. 2022

Biotechnology Reports

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Highlights A platform for rapid expression of genes through agro-infiltration is a suitable alternative to the laborious process of generating stable transgenic lines for producing recombinant immunogenic antigens in plants. This study has successfully developed and demonstrated a reproducible transformation protocol for rapid expression of Helicobacter pylori recombinant CagA, VacA and NapA antigens in Nicotiana benthamiana . This is the first study of recombinant expression CagA, VacA and NapA gene of Helicobacter pylori in Nicotiana benthamiana via syringe-assisted A grobacterium infiltration. A syringe infiltration of a four to five weeks old Nicotiana benthamiana plant with Agrobacterium tumefaciens subtype EHA105 was optimal to produce the maximum mRNA levels of CagA, VacA and NapA mRNA in leaf at the third-day post-Agro-infiltration.

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Section: Discussionmentioning

confidence: 99%