Production of a new second generation biodiesel with a low cost lipase derived from Thermomyces lanuginosus: Optimization by response surface methodology

Verdugo, Cristóbal; Luna, Diego; Posadillo, Alejandro; Sancho, Enrique; Rodrı́guez, S.; Bautista, Felipa M.; Luque, Rafael; Marinas, J. M.; Romero, Antonio A.

doi:10.1016/j.cattod.2010.12.028

Cited by 60 publications

(88 citation statements)

References 30 publications

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“…Thus, reactions were carried out with 0.8 g of 10 wt% supported KF on the three inorganic solids, Al 2 O 3 , ZnO and MgO, at 65 ºC reaction temperature with 12 mL of sunflower oil (32 cSt, kinematic viscosity) and 2.43 mL of methanol. According to the results, with all the three KF supported catalysts studied, under the indicated experimental conditions, were obtained similar results as those achieved in the enzymatic processes [19][20][21][22][23][24], where instead of glycerol are obtained the monoacylglycerol that are soluble in the FAME mix. The experimental conditions currently applied were clearly softer than those required for the production of conventional biodiesel with supported KF catalysts [29][30][31][32][33][34][35][36].…”

Section: Influence Of the Inorganic Solid Used As Support Of Kf On Prsupporting

confidence: 73%

“…As carrier gas helium is used, with a flow of 1.5 ml/min, it has been applied a heating ramp from 50 °C to 200 °C at a rate of 7 °C/min, followed by another ramp from 200 °C to 360 °C at a rate of 15 ºC/min, maintaining the oven temperature at 360 °C for 10 minutes using as internal standard n-hexadecane (cetane) to quantify the content of methyl esters and glycerides (-mono, di and triglycerides) with the help of some commercial standard fatty acid esters, respectively. This method allows us to obtain a complete analysis of the sample in a single injection and in a time not higher than 65 minutes, which simplifies the process and increases the speed of analysis [19][20][21][22][23][24].…”

Section: Methodsmentioning

confidence: 99%

“…It correlates to the movement restriction suffered by the liquid flow, as a result of its molecules internal friction, depending on their viscosity. From the flow time, t, in seconds, the kinematic viscosity (mm2/s or centistokes, cSt) can be obtained from the equation: C x t = viscosity, where C is the constant calibration of the measuring system in mm 2 /s x s, which is given by the manufacturer (0.040350 mm2 s -1 , at 40 ºC) and t the flow time in seconds [19][20][21][22][23][24]. The sample, previously centrifuged at 3500 rpm for 10 minutes, is immersed in a thermostatic bath at 40 °C for 15 minutes, making sure that the temperature is stable.…”

Section: Viscosity Measurementsmentioning

confidence: 99%

“…This enzymatic method has shown promising results [19][20][21][22][23][24] in the obtaining of a new biofuel, named Ecodiesel-100, patented by University of Cordoba [22] using pig pancreatic lipase (PPL) to obtain the enantioselective ethanolysis of sunflower oil. This method takes advantage of the 1.3-selective nature of many lipases, which allows stopping the process in the second step of the alcoholysis reaction to obtain a mixture of two moles of FAEE and one MG.…”

Section: Introductionmentioning

confidence: 99%

“…In this respect it is interesting to point out in this respect that according to recent studies the presence of MG enhances the lubricating properties of the biofuel [25,26]. So far this process is being developed not only with PPL but also with some microbial lipases, both in free and immobilized forms [19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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Production of a Biofuel that Keeps the Glycerin as Monoglycerides by Using Supported KF as Heterogeneous Catalyst

Calero

Cumplido

Luna

et al. 2014

Proceedings of 1st International E-Conference on Energies

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This study describes the results obtained in the synthesis of a biofuel that avoids the glycerol production by applying supported KF as alkaline heterogeneous catalyst, to generate two moles of fatty acid methyl esters and one mole of monoglyceride, from one mol of triglyceride. In this respect, the selective transesterification process of sunflower oil with methanol was carried out with KF10 wt % supported on three different solids, Al 2 O 3 , ZnO and MgO. The standard experimental conditions employed in the heterogeneous selective methanolysis reaction were: 12 mL of sunflower oil, 2.7 mL of methanol, 0.8 g of catalyst, at 65 °C temperature and one hour of reaction time. In all cases were obtained 100% conversion, with high selectivity values, greater than 90%, and quite suitable viscosity values, 4.5-8.5 cSt. In this way, the best catalytic behavior in the first use was obtained by using Al 2 O 3 as support. However, although in the five consecutive reuses all exhibited a continuous decrease in the catalytic activities; the lower one was for KF catalyst using MgO as support. In summary, these three KF supported catalysts have OPEN ACCESSEnergies 2013, 6 2 resulted to be very suitable to obtain a new biofuel, similar to conventional biodiesel, applicable to diesel engines.

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Section: Influence Of the Inorganic Solid Used As Support Of Kf On Prsupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Viscosity Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Production of a Biofuel that Keeps the Glycerin as Monoglycerides by Using Supported KF as Heterogeneous Catalyst

Calero

Cumplido

Luna

et al. 2014

Proceedings of 1st International E-Conference on Energies

Self Cite

View full text Add to dashboard Cite

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The use of alternative solvents in enzymatic biodiesel production: a review

Taher¹,

Al‐Zuhair

2016

Biofuels Bioprod Bioref

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Biodiesel produced from oil‐rich feedstocks is known as a green replacement for conventional petroleum diesel. Transesterification is the common method used for biodiesel production. The enzymatic biodiesel production, using lipases, has recently been receiving extensive attention, due to its advantages over conventional alkali catalyzed process. The lipases can work at mild operating conditions, thus requiring less energy, with the ability to catalyze both triglycerides and free fatty acids (FFAs) from different oil sources, requiring fewer post‐production steps.Among the process challenges that face this process is the inhibition of the lipase with excessive use of alcohols, resulting in a decrease in biodiesel yield. The use of organic solvents, as a reaction medium, becomes essential in this case. However, most organic solvents are toxic and volatile with harmful impacts on the environmental. In addition, with the use of volatile organic solvents, an additional unit is required to separate them from the products, for the purification of the product and recycling of the solvent. In this paper, the prospect of using alternative solvents, specifically supercritical carbon dioxide (SC‐CO2) and ionic liquids (ILs), in enzymatic biodiesel production is presented. The properties of these alternative solvents, their advantages over organic solvents, factors affecting lipases activity and stability in these solvents, and the challenges facing process commercialization are discussed. © 2016 Society of Chemical Industry and John Wiley & Sons, Ltd

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Lipase‐catalyzed process for biodiesel production: Protein engineering and lipase production

Hwang

Yuan

et al. 2013

Biotech & Bioengineering

101

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Biodiesel is an environment-friendly and renewable fuel produced by transesterification of various feedstocks. Although the lipase-catalyzed biodiesel production has many advantages over the conventional alkali catalyzed process, its industrial applications have been limited by high-cost and low-stability of lipase enzymes. This review provides a general overview of the recent advances in lipase engineering, including both protein modification and production. Recent advances in biotechnology such as in protein engineering, recombinant methods and metabolic engineering have been employed but are yet to impact lipase engineering for cost-effective production of biodiesel. A summary of the current challenges and perspectives for potential solutions are also provided.

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Production of a new second generation biodiesel with a low cost lipase derived from Thermomyces lanuginosus: Optimization by response surface methodology

Cited by 60 publications

References 30 publications

Production of a Biofuel that Keeps the Glycerin as Monoglycerides by Using Supported KF as Heterogeneous Catalyst

Production of a Biofuel that Keeps the Glycerin as Monoglycerides by Using Supported KF as Heterogeneous Catalyst

The use of alternative solvents in enzymatic biodiesel production: a review

Lipase‐catalyzed process for biodiesel production: Protein engineering and lipase production

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