Production of a monoclonal antibody against of muskellunge (Esox masquinongy) IgM heavy chain and its use in development of an indirect ELISA for titrating circulating antibodies against VHSV-IVB

Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (Clarias macrocephalus): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM+ Cells in Clarias Catfish

“…Our findings revealed that the developed IgMHCµ 1 mAb showed both high sensitivity and specificity to the target IgMHCµ 1 protein in all of the tested immunological assays in catfish compared to previously-reported mAbs of teleosts [17,19,21,22,39]. These properties minimize the possibility of false negative and false positive signals among the obtained results.…”

“…The fish IgM, D, and T/Z classes refer to the protein products of the isotypes µ, δ, and τ/ζ, respectively, which correspond to their associated constant genes [10,11,26]. Over the past decade, in this new era of immunological research, monoclonal antibodies (mAbs) have been recognized as a novel technology that can be beneficial tools for the study of catfish; mAbs can be used as cell-specific markers, for the quantification of target cells in immunoassays, as diagnostic reagents in pathogen detection, and for therapy [19][20][21]. Consequently, mAbs specific to IgM molecules have been developed and produced for determining health status in various important economic fish species, including common carp (Cyprinus carpio), channel catfish (Ictalurus punctatus), cod (Gadus morhua), eel (Anguilla Anguilla), Japanese flounder (Paralichthys olivaceus), red drum (Sciaenops ocellatus), Atlantic salmon (Salmo salar), sea bream (Sparus aurata), sea bass (Dicentrachus labrax), rainbow trout (Oncorhynchus mykiss), and turbot (Scophthalmus maximus) [10,11,[26][27][28][29][30][31][32][33].…”

“…In recent decades, molecular studies of the Ig gene in catfish have been important in both basic and applied research. Their applications are especially useful in fish cultivation [17,18], in pathogen detection where they provide diagnostic reagents, and for therapy [19][20][21]. Furthermore, such studies can facilitate improving the aquaculture of catfish by selecting for specific disease-resistance traits.…”