Production of a monoclonal antibody specific for seminomas and dysgerminomas.

Abstract

A monoclonal antibody (M2A, IgG2a) was produced against a cultured human ovarian epithelial adenocarcinoma cell line, HEY. Monoclonal antibody M2A reacted with a glycoprotein of molecular weight 40,000 on the surface of HEY cells. The affinity constant of the monoclonal antibody M2A for HEY cells was 109 M-1, and the number of binding sites on HEY cells was 2 x 104 per cell. The monoclonal antibody produced positive immunoperoxidase staining of fetal (but not adult) testis and of seminomas and dysgerminomas but did not stain various normal adult tissues or other gonadal or extragonadal tumors. Monoclonal antibody M2A may be useful for confirming a histological diagnosis of seminoma and dysgerminoma.

D2-40 immunohistochemistry in the differential diagnosis of seminoma and embryonal carcinoma: a comparative immunohistochemical study with KIT (CD117) and CD30

“…16 Among normal tissues, this antigen has been shown to be present in fetal germ cells, lymphatic endothelium, and mesothelial cells. [17][18][19] Initial immunohistochemical investigations performed on frozen tissue specimens using a monoclonal antibody produced against a human ovarian epithelial adenocarcinoma cell line demonstrated M2A antigen expression in all seminomas and seminomatous components of mixed germ cell tumors, but in none of the nonseminomatous germ cell tumors studied. 17,18 In the present investigation utilizing the D2-40 antibody on paraffin-embedded tissue samples, immunoreactivity was observed in all cases of seminoma, in agreement with the distribution of the M2A antigen established by previous studies using immunohistochemical analysis on frozen sections.…”

“…[17][18][19] Initial immunohistochemical investigations performed on frozen tissue specimens using a monoclonal antibody produced against a human ovarian epithelial adenocarcinoma cell line demonstrated M2A antigen expression in all seminomas and seminomatous components of mixed germ cell tumors, but in none of the nonseminomatous germ cell tumors studied. 17,18 In the present investigation utilizing the D2-40 antibody on paraffin-embedded tissue samples, immunoreactivity was observed in all cases of seminoma, in agreement with the distribution of the M2A antigen established by previous studies using immunohistochemical analysis on frozen sections. 17,18 However, in contrast to the results of these aforementioned reports indicating an absence of M2A in nonseminomatous germ cell tumors, D2-40 immunoreactivity was observed in four of 14 (29%) samples of embryonal carcinoma in the present study.…”

“…16 The M2A antigen is present in fetal germ cells of the testis, as well as lymphatic endothelial cells and mesothelial cells. [17][18][19] In the context of germ cell neoplasia, the distribution of the M2A antigen has been shown to be largely restricted to intratubular germ cell neoplasia and seminoma, with limited to absent expression in non seminomatous germ cell tumors. [16][17][18][19][20][21] The selective expression of the M2A antigen in seminoma suggests potential use of the D2-40 antibody for distinguishing this particular tumor from embryonal carcinoma.…”

“…[17][18][19] In the context of germ cell neoplasia, the distribution of the M2A antigen has been shown to be largely restricted to intratubular germ cell neoplasia and seminoma, with limited to absent expression in non seminomatous germ cell tumors. [16][17][18][19][20][21] The selective expression of the M2A antigen in seminoma suggests potential use of the D2-40 antibody for distinguishing this particular tumor from embryonal carcinoma. In the present study, the immunohistochemical expression of D2-40 was evaluated in testicular germ cell tumors and compared with CD30 and KIT to determine the relative usefulness of this particular marker in discriminating between seminoma and embryonal carcinoma.…”

D2-40 immunohistochemical analysis of pediatric vascular tumors reveals positivity in kaposiform hemangioendothelioma

“…12 The carbohydrate structure of the antigen recognized by D2-40 is common for mucin-type glycoproteins that are widely expressed in normal and tumor cells. 20 Thus, although M2A was initially identified in fetal gonocytes, germ-cell tumors, and prepubertal Sertoli cells, [21][22][23] it is also present in a number of human cancer cell lines, including osteosarcoma and transitional cell carcinoma. 12 The broad application of D2-40 immunohistochemistry has established reactivity to multiple cell lineages and tumors such as mesothelioma, ovarian serous carcinoma, schwannoma, meningioma, synovial sarcoma, gastro-intestinal stromal tumor, leiomyosarcoma and others.…”