Production of a Metal-Binding Exopolysaccharide by Paenibacillus jamilae Using Two-Phase Olive-Mill Waste as Fermentation Substrate

Morillo, José A.; Aguilera, Margarita; Ramos‐Cormenzana, A.; Monteoliva‐Sánchez, Mercedes

doi:10.1007/s00284-005-0438-7

Cited by 69 publications

(27 citation statements)

References 18 publications

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“…In the case of the use of TPOMW as substrate, maximal EPS yield (2 g l −1 ) was obtained in cultures prepared with an aqueous extract of 20% TPOMW (w/v). An inhibitory effect was observed on growth and EPS production when TPOMW concentration was increased (Morillo et al 2006). This EPS produced by P. jamilae through fermentation of OMWs has been investigated in relation to its potential application as a biofilter of heavy-metal-contaminated water (Morillo et al 2008b).…”

Section: Biopolymers and Enzymesmentioning

confidence: 99%

“…The development of effective methods to purify/isolate the enzymes and biopolymers from the bulk fermentation is an important point in order to scale the processes to the industrial scale. The required methodologies are specific of the enzyme/technology considered and generally involves further steps of concentration, precipitation and chromatography (Morillo et al 2006;D'Annibale et al 2004b).…”

Section: Biopolymers and Enzymesmentioning

confidence: 99%

See 1 more Smart Citation

Bioremediation and biovalorisation of olive-mill wastes

Morillo

Antízar-Ladislao

Monteoliva‐Sánchez

et al. 2009

Appl Microbiol Biotechnol

192

102

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Olive-mill wastes are produced by the industry of olive oil production, which is a very important economic activity, particularly for Spain, Italy and Greece, leading to a large environmental problem of current concern in the Mediterranean basin. There is as yet no accepted treatment method for all the wastes generated during olive oil production, mainly due to technical and economical limitations but also the scattered nature of olive mills across the Mediterranean basin. The production of virgin olive oil is expanding worldwide, which will lead to even larger amounts of olive-mill waste, unless new treatment and valorisation technologies are devised. These are encouraged by the trend of current environmental policies, which favour protocols that include valorisation of the waste. This makes biological treatments of particular interest. Thus, research into different biodegradation options for olive-mill wastes and the development of new bioremediation technologies and/or strategies, as well as the valorisation of microbial biotechnology, are all currently needed. This review, whilst presenting a general overview, focusses critically on the most significant recent advances in the various types of biological treatments, the bioremediation technology most commonly applied and the valorisation options, which together will form the pillar for future developments within this field.

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Section: Biopolymers and Enzymesmentioning

confidence: 99%

Section: Biopolymers and Enzymesmentioning

confidence: 99%

Bioremediation and biovalorisation of olive-mill wastes

Morillo

Antízar-Ladislao

Monteoliva‐Sánchez

et al. 2009

Appl Microbiol Biotechnol

192

102

View full text Add to dashboard Cite

show abstract

“…Paenibacillus polymyxa (P13), a strain isolated from fermented sausages, produced EPS (mannose polymer) under hyperosmotic stress which showed strong ability to bind Cu(II) [66] and the maximum binding capacity was double than those reported for other members of the Bacillaceae [56,67]. Likewise, Paenibacillus jamilae CECT 5266 grown in aqueous extracts of two-phase olive mill waste (TPOMW) produced EPS capable of absorbing heavy metals in the following order from a multi-metal sorption system: Pb>Cd>Cu>Zn>Ni>Co. Lead was preferentially complexed by the polymer (228 mg g -1 ) [68] . Cyanobacterial cells possessing a thick capsular polysaccharide investment outside the cell contain large number of binding sites for trapping metal ions and are quite promising in heavy metal bioremediation.…”

Section: Eps and Heavy Metal Immobilizationmentioning

confidence: 99%

Microbial extracellular polymeric substances: central elements in heavy metal bioremediation

2008

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Extracellular polymeric substances (EPS) of microbial origin are a complex mixture of biopolymers comprising polysaccharides, proteins, nucleic acids, uronic acids, humic substances, lipids, etc. Bacterial secretions, shedding of cell surface materials, cell lysates and adsorption of organic constituents from the environment result in EPS formation in a wide variety of free-living bacteria as well as microbial aggregates like biofi lms, biofl ocs and biogranules. Irrespective of origin, EPS may be loosely attached to the cell surface or bacteria may be embedded in EPS. Compositional variation exists amongst EPS extracted from pure bacterial cultures and heterogeneous microbial communities which are regulated by the organic and inorganic constituents of the microenvironment. Functionally, EPS aid in cell-to-cell aggregation, adhesion to substratum, formation of fl ocs, protection from dessication and resistance to harmful exogenous materials. In addition, exopolymers serve as biosorbing agents by accumulating nutrients from the surrounding environment and also play a crucial role in biosorption of heavy metals. Being polyanionic in nature, EPS forms complexes with metal cations resulting in metal immobilization within the exopolymeric matrix. These complexes generally result from electrostatic interactions between the metal ligands and negatively charged components of biopolymers. Moreover, enzymatic activities in EPS also assist detoxifi cation of heavy metals by transformation and subsequent precipitation in the polymeric mass. Although the core mechanism for metal binding and / or transformation using microbial exopolymer remains identical, the existence and complexity of EPS from pure bacterial cultures, biofi lms, biogranules and activated sludge systems differ signifi cantly, which in turn affects the EPS -metal interactions. This paper presents the features of EPS from various sources with a view to establish their role as central elements in bioremediation of heavy metals.

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“…The common mechanisms of heavy metal resistance include ATPase efflux, oxidation/reduction, accumulation or immobilization in exopolysaccharides, precipitation, intracellular sequestration, and volatilization by the addition of methyl or ethyl groups (8,15,33,36,39,40,42,43). Specifically, extracellular polysaccharides from Paenibacillus species have been used as a biosorbent for lead, sorbing over 300 mg Pb/g (29,30). Many studies have documented lead toxicity as low (with higher MIC levels) compared to that of heavy metals such as Co, Cd, As, Cu, and Zn (13,27,34,41,46).…”

mentioning

confidence: 99%