Production of a full chimeric mouse x pig anti-porcine DEC205 receptor recombinant antibody

Bustamante-Córdova, Lorena; Melgoza-González, Edgar Alonso; Parra-Sánchez, Héctor; Reséndiz-Sandoval, Mónica; Burgara-Estrella, Alexel; Hernández, Jesús

doi:10.1016/j.jim.2020.112911

Cited by 2 publications

(4 citation statements)

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“…Previously, we evaluated the rAb ZH9F7 half-life in the bloodstream. The obtained results showed that after 8 h of intravenous injection, rAb ZH9F7 in sera significantly decreased [ 17 ]. This finding may be due to receptor-mediated endocytosis, a mechanism that we confirmed; therefore, rAb ZH9F7 was not found to be free in circulation but was possibly internalized in DEC205 + blood cells or other DEC205 + cells in tissues.…”

Section: Discussionmentioning

confidence: 99%

“…The main advantage of using chimeric-hybrid antibodies for which the recombinant chimeric mouse × pig antibody rAb ZH9F7 was designed and expressed is in reducing the antigenicity of murine monoclonal antibodies applied in other species such as humans or pigs [ 17 ]. Therefore, when we used rAb ZH9F7 without Cap antigen for in vitro stimulation experiments, we confirmed that the induction of IFN-γ-secreting cells was not significant in response to rAbZH9F7 itself.…”

Section: Discussionmentioning

confidence: 99%

“…The chimeric mouse × pig anti-DEC205 antibody design was performed as previously reported by Bustamante-Córdova et al [ 17 ]. The synthesis of genes encoding SwHCZH9F7-Cap and SwLCZH9F7 was performed by GenScript (Piscataway, NJ, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Previously, our research group developed a recombinant mouse × pig chimeric antibody against porcine DEC205 receptor (rAb ZH9F7), and antigens from PRRSV were targeted by intradermal application [ 16 , 17 ]. The obtained results showed that the system was immunogenic with limited protective immunity.…”

Section: Introductionmentioning

confidence: 99%

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Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells

et al. 2022

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Dendritic cell (DC) targeting by DEC205+ cells effectively promotes the internalization of antigens that may trigger a specific immune response. In this study, we evaluated the ability of a recombinant antibody, anti-DEC205 (rAb ZH9F7), to trigger cellular endocytosis in subpopulations of DCs and targeted cells after intradermal injection and subsequent migration toward lymph nodes. Furthermore, the cellular immune response was evaluated in pigs after intradermal application of the antigenized rAb ZH9F7 combined with porcine circovirus type 2 cap antigen (rAb ZH9F7-Cap). We demonstrated that rAb ZH9F7 recognized conventional type 1 and 2 DCs from the blood and skin and monocytes. It promoted receptor-mediated endocytosis and migration of cDCs and moDCs toward regional lymph nodes. Intradermal application of rAb ZH9F7-Cap induced a higher frequency of IFN-γ-secreting CD4+CD8+ T lymphocytes and antibodies against Cap protein than that in the control group. In conclusion, the rAb ZH9F7-Cap system promoted the target of skin cDC1 and cDC2, provoking migration to the regional lymph nodes and inducing a Th1 response, as evidenced by the proliferation of double-positive CD4+CD8+ T cells, which correlates with an enhanced ability to target the cDC1 subset both in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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