Production of a bioactive sweetener steviolbioside via specific hydrolyzing ester linkage of stevioside with a β-galactosidase

Chen, Junming; Ding, Li; Sui, Xiao-chen; Xia, Yongmei; Wan, Hui-da; Lu, Tong

doi:10.1016/j.foodchem.2015.09.035

Cited by 23 publications

(17 citation statements)

References 20 publications

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“…Our results here also demonstrated no impact of dietary stevia on the kinetics of pancreatic acinar carcinoma. This is seemingly in contradiction with in vitro data from several laboratories, which demonstrate that various S. rebaudiana extracts have antiproliferative and proapoptotic properties on cancer cell lines ( 29 – 31 ), including on the pancreatic cancer cell lines MiaPaCa-2 ( 32 ) and BxPC-3 ( 33 ). However, when extrapolating to the physiological context, the process of digestion needs to be taken into account.…”

Section: Discussioncontrasting

confidence: 65%

No Effect of Dietary Aspartame or Stevia on Pancreatic Acinar Carcinoma Development, Growth, or Induced Mortality in a Murine Model

et al. 2017

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Pancreatic cancer has an extremely poor prognosis, largely due to a poor record for early detection. Known risk factors for pancreatic cancer include obesity, diet, and diabetes, implicating glucose consumption and regulation as a key player. The role of artificial sweeteners may therefore be pertinent to disease kinetics. The oncogenic impact of artificial sweeteners is a highly controversial area. Aspartame, one of the most studied food additives, is widely recognized as being generally safe, although there are still specific areas where research is incomplete due to study limitations. Stevia, by contrast, has been the subject of relatively few studies, and the potential health benefits are based on extrapolation rather than direct testing. Here, we used longitudinal tracking of pancreatic acinar carcinoma development, growth, and lethality in a sensitized mouse model. Despite exposure to aspartame and stevia from the in utero stage onward, we found no disease modification activity, in either direction. These results contribute to the data on aspartame and stevia safety, while also reducing confidence in several of the purported health benefits.

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Section: Discussioncontrasting

confidence: 65%

No Effect of Dietary Aspartame or Stevia on Pancreatic Acinar Carcinoma Development, Growth, or Induced Mortality in a Murine Model

et al. 2017

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“…Regarding hydrolases, although they have been used extensively in the literature for various transglycosylation reactions, studies reporting the transglycosylation of steviol glycosides are sparse. β-Glucosidases and β-galactosidases have been used mainly for the hydrolysis of stevioside, aiming at the production of less glucosylated products ( Ko et al, 2012 , 2013 ; Nguyen et al, 2014 ; Chen et al, 2016 ), while a few studies report the transgalactosylation by β-galactosidases ( Kitahata et al, 1989 ). According to the literature, β-galactosidases usually transgalactosylate their acceptor substrates ( Irazoqui et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

Zerva

Chorozian

Kritikou

et al. 2021

Front. Bioeng. Biotechnol.

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Stevia rebaudiana Bertoni is a plant cultivated worldwide due to its use as a sweetener. The sweet taste of stevia is attributed to its numerous steviol glycosides, however, their use is still limited, due to their bitter aftertaste. The transglycosylation of steviol glycosides, aiming at the improvement of their taste, has been reported for many enzymes, however, glycosyl hydrolases are not extensively studied in this respect. In the present study, a β-glucosidase, MtBgl3a, and a β-galactosidase, TtbGal1, have been applied in the transglycosylation of two steviol glycosides, stevioside and rebaudioside A. The maximum conversion yields were 34.6 and 33.1% for stevioside, while 25.6 and 37.6% were obtained for rebaudioside A conversion by MtBgl3a and TtbGal1, respectively. Low-cost industrial byproducts were employed as sugar donors, such as cellulose hydrolyzate and acid whey for TtbGal1- and MtBgl3a- mediated bioconversion, respectively. LC-HRMS analysis identified the formation of mono- and di- glycosylated products from stevioside and rebaudioside A. Overall, the results of the present work indicate that both biocatalysts can be exploited for the design of a cost-effective process for the modification of steviol glycosides.

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“…Among them, β-galactosidases from K. lactis and A. niger have shown high specific activity for the glycosyl ester linkage hydrolysis of Ste, producing steviolbioside as the primary product. A steviolbioside yield of 96% has been obtained at 25 mg Ste/mL, 40°C, pH 7.0, and 9000 U/g Ste for 12 h (Chen et al, 2016).…”

Section: Production Of Steviolbiosidementioning

confidence: 99%

“…Steviolbioside 13-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxyl]kaur-16-en-18oic acid] is a natural sweetener found in S. rebaudiana leaves in rare amounts (Ibrahim et al, 2014). Synthetic methods for steviolbioside such as alkaline and enzymatic hydrolysis of Ste have been investigated (Ko et al, 2013;Chen et al, 2016). Steviolbioside is a byproduct in the production of steviol from Ste using purified β-glucosidase obtained by P. decumbens naringinase (Ko et al, 2013).…”

Section: Production Of Steviolbiosidementioning

confidence: 99%

“…Steviolbioside is a byproduct in the production of steviol from Ste using purified β-glucosidase obtained by P. decumbens naringinase (Ko et al, 2013). Chen et al have screened the following six commercial or homemade glycosidases and lipase: β-galactosidase from Kluyveromyces lactis (Maxilact LG 2000), β-galactosidase from S. solfataricus, β-glucosidase from A. niger (Novozymes), β-glucosidase from Penicillium multicolor (Aromase), Candida antarctica (Novozyme 435), and Rhizomucor miehei (Lipozyme RM IM) (Chen et al, 2016). Among them, β-galactosidases from K. lactis and A. niger have shown high specific activity for the glycosyl ester linkage hydrolysis of Ste, producing steviolbioside as the primary product.…”

Section: Production Of Steviolbiosidementioning

confidence: 99%

See 1 more Smart Citation

Enzymatic Production of Steviol Glucosides Using β-Glucosidase and Their Applications

Nguyen

Seo

Kwak

et al. 2019

Enzymes in Food Biotechnology

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Production of a bioactive sweetener steviolbioside via specific hydrolyzing ester linkage of stevioside with a β-galactosidase

Cited by 23 publications

References 20 publications

No Effect of Dietary Aspartame or Stevia on Pancreatic Acinar Carcinoma Development, Growth, or Induced Mortality in a Murine Model

No Effect of Dietary Aspartame or Stevia on Pancreatic Acinar Carcinoma Development, Growth, or Induced Mortality in a Murine Model

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

Enzymatic Production of Steviol Glucosides Using β-Glucosidase and Their Applications

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