Production of 4-Deoxy-L-erythro-5-Hexoseulose Uronic Acid Using Two Free and Immobilized Alginate Lyases from Falsirhodobacter sp. Alg1

Abstract

Falsirhodobacter sp. alg1 expresses two alginate lyases, AlyFRA and AlyFRB, to produce the linear monosaccharide 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) from alginate, metabolizing it to pyruvate. In this study, we prepared recombinant AlyFRA and AlyFRB and their immobilized enzymes and investigated DEH production. Purified AlyFRA and AlyFRB reacted with sodium alginate and yielded approximately 96.8% DEH. Immobilized AlyFRA and AlyFRB were prepared using each crude enzyme solution and κ-carrageenan, and immobilized enzyme reuse in batch reactions and DEH yield were examined. Thus, DEH was produced in a relatively high yield of 79.6%, even after the immobilized enzyme was reused seven times. This method can produce DEH efficiently and at a low cost and can be used to mass produce the next generation of biofuels using brown algae.

Utilization of Macroalgae for the Production of Bioactive Compounds and Bioprocesses Using Microbial Biotechnology

“…Tanaka et al examined DEH production using free and immobilized alginate lyases, endo-type AlyFRA, and exo-type AlyFRB from Falsirhodobacter sp. alg1 [ 90 ].…”

“…The immobilization rates of AlyFRA and AlyFRB increased as the concentration of κ-carrageenan increased, and their κ-carrageenan gels were less fragile. Immobilized enzymes prepared with 4.0% ( w / v ) κ-carrageenan completely degraded the substrate and produced DEH in the seventh batch reaction [ 90 ]. Considering these facts, the immobilization of AlyFRA and AlyFRB can effectively and economically produce large amounts of DEH from sodium alginate.…”

Identification and characterization of a critical loop for the high activity of alginate lyase VaAly2 from the PL7_5 subfamily

“…In addition, the depolymerization of alginates by alginate lyases is more efficient due to the high enzyme activity and substrate specificity. Therefore, alginate lyases have been widely used in the complete degradation of brown algae and the production of AOSs (Cheng et al, 2020;Gao et al, 2021;Mrudulakumari Vasudevan et al, 2021;Cao et al, 2022;Lu et al, 2022;Tanaka et al, 2022;Li et al, 2023a;Takasuka et al, 2023). Moreover, the decomposition of the bacterial polysaccharide biofilm by alginate lyases is also a potential treatment for cystic fibrosis (Inoue, 2018;Dharani et al, 2020;Gao et al, 2021;Martin et al, 2021).…”