Production of 3-Vinylcatechol and Physiological Properties ofPseudomonasLF-3, Which Can Assimilate Styrene in a Two-phase (Solvent-Aqueous) System

Celebrating 20 Years of SYNLETT - Special Account On the Merits of Biocatalysis and the Impact of Arene cis-Dihydrodiols on Enantioselective Synthesis

“…It is, however, less efficient than the biocatalytic syn-thesis of catechols from glucose reported by Frost 38 and Yoshida. 39 The major advantage of using whole-cell fermentation rather than isolated or immobilized dioxygenases in the preparation of the cis-dihydrodiols is the fact that no cofactors such as NADH are required for reductive steps in the cycle. Oxidoreductases in general perform their function along a complex electron-transport cycle and stoichiometric amounts of cofactors would have to be either added to the reaction involving isolated enzymes or various cofactor recycling loops established for each particular process.…”