Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene

Abstract

In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11α-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles.

Characterization of metal removal of immobilized Bacillus strain CR-7 biomass from aqueous solutions

“…A higher rotation speed, 200 rpm, could not cause cracks or ruptures of the adsorbent beads. A similar result was that increase of rotation speed to more than 150 rpm caused no considerable change in production of 11␣-hydroxyprogesterone by immobilized Aspergillus terreus biomass [45].…”

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level Level

“…c d e f The economics of the immobilization process depends on the lifespan of the biocatalyst immobilized (40). In this study, the immobilized cells were stored at 4 °C and lipase production was found to be constant even after 6 months of storage (data not shown).…”

Efficient hydroxylation of functionalized steroids by Colletotrichum lini ST-1

“…Therefore, microbial transformation has been widely used in the conversion and biosynthesis of steroid pharmaceuticals. in the conversion of steroids [17][18][19][20][21]. The strain Colletotrichum lini ST-1, as we mentioned in previous study [22], possessed an active monooxygenase which could transform DHEA (10 g/L) into 7␣-OH-DHEA and 7␣,15␣-diOH-DHEA ( Fig.…”