Production of 1,3-propanediol from glycerol via fermentation by Saccharomyces cerevisiae

Tabah, Betina; Varvak, Alexander; Pulidindi, Indra Neel; Foran, Elizabeth; Banin, Ehud; Gedanken, Aharon

doi:10.1039/c6gc00125d

Cited by 41 publications

(17 citation statements)

References 84 publications

(95 reference statements)

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“…Presently, the 1,3‐PDO synthesis mainly follows chemical and fermentative routes. Compared with the chemical method, the alternative fermentative route which does not require high temperature, pressure, the use of expensive chemical catalysts and toxic organic solvents, is a more environmentally friendly process . Furthermore, compared with some other aerobic fermentation processes, due to the anaerobic and unsterilized culture requirements, the biotechnological conversion of crude glycerol (industrial waste glycerol) to 1.3‐PDO through Clostridium butyricum is a more green process which will save more energy and cost …”

Section: Introductionmentioning

confidence: 99%

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

et al. 2018

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In order to obtain the excellent 1,3-propanediol (1,3-PDO) producer from wild-type Clostridium butyricum, adaptive evolution was carried out to select the strain for fast growth. The most significant change was that fermentation time decreased from 36 h to 20 h after adaptive evolution. Thus, it led to the corresponding volumetric productivity of 1,3-PDO increasing from 0.97 to 2.14 (g/LÁh −1 ) which increased by 114%. Adaptive evolution was also applied to butyric acid tolerant strain selected based on the fast-growing one through a simple equipment. 1,3-PDO concentration increased from 40.28 to 66.23 g/L through fed-batch fermentation by butyric acid tolerant strain compared with the fast-growing one. In addition, the endpoint strain was successfully and steadily used in the 50-L scale fermentation. Thus, adaptive evolution is an excellent strategy which can help us select the fast-growing strain and reduce the negative effect from substrate and metabolite inhibition. (a) the morphology of Clostridium butyricum in the initial stage of Gen 0 adaptive evolution process. (b) The morphology of Clostridium butyricum in the last stage of Gen 0 adaptive evolution process. (c) The effect of 5 g/L butyric acid and acetic acid to the growth of Gen 4 strain. (d) Different growing status of Gen 0, Gen 1, Gen 2, Gen 3, and Gen 4 strain in seed culture contained 70 g/L glycerol. The "XMU" were written on the back of the each bottles. [Color figure can be viewed at wileyonlinelibrary.com] Figure 5. Time course of Gen 7 strain fermentation on 50-L scale. (a, b) Represent the concentrations of glycerol, 1,3-PDO (g/L), acids and OD in batch and fed-batch fermentation process.[Color figure can be viewed at wileyonlinelibrary.com]

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Section: Introductionmentioning

confidence: 99%

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

et al. 2018

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“…Gas produced by C. acetobutylicum is a mixture of hydrogen (H2) and carbon dioxide (CO2) that can be further valorised. The hydrolysed bio-oils fractions rich in glucose could be converted following this same approach by other bacteria or yeast such as glutamic acid, 3hydroxypropionic acid, 1,3-propanodiol, succinic acid, isoprene, farnesene, itaconic acid and lactic acid [73][74][75].…”

Section: Integration Of Thermochemical and Biological Conversionmentioning

confidence: 99%

Fermentation of cellulose pyrolysis oil by a Clostridial bacterium

Buendia-Kandia

Greenhalf

Barbiero

et al. 2020

Biomass and Bioenergy

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The coupling of thermochemical and biological conversion of biomass is a promising strategy to produce chemicals in future integrated biorefineries. Indeed, thermochemical conversion such as pyrolysis is a fast process without any solvent or enzyme for the depolymerization of biomass. In this work, cellulose was pyrolyzed to produce sugars which have been then fermented by bacteria (Clostridium acetobutylicum) to produce acetone and butanol. This type of bacteria presents an interesting biological platform: it is resilient, easily up-scalable and Clostridium can be genetically engineered to target various other chemicals. Pyrolysis of cellulose was performed in a continuous fluidized bed reactor equipped with a staged condensation system, including a warm electrostatic precipitator. Different bio-oil fractions rich in levoglucosan (LVG) and with different concentrations in inhibitors for the fermentation stage were produced. LVG was found to be nonfermentable by C. acetobutylicum. Therefore, the bio-oil fractions were hydrolysed to obtain fermentable glucose. The mechanisms of acid hydrolysis (with diluted H2SO4) of LVG and cellobiosan have been revealed by high resolution mass spectrometry. The microorganisms were not able to grow with all hydrolysed bio-oil fractions depending on the concentration in inhibitors (aldehydes and organic acids). The fractions rich in LVG (and then glucose) lead to normal bacterial growth and normal fermentation products pattern without the need of detoxification. These results show the importance of a pyrolysis process with a staged condensation as a preliminary step for fermentation. It opens the road to production of various cellulose-derived chemicals by bacteria.

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“…Among the various osmophilic yeast capable of producing erythritol, Yarrowia lipolytica is particularly promising. This yeast is best known for its ability to produce citric acid and lipid-based chemicals at high titers [ 24 – 28 ]. It is also a GRAS organism with good genetic tools [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of the oleaginous yeast Yarrowia lipolytica PO1f for production of erythritol from glycerol

Jagtap

Bedekar

Liu

et al. 2021

Biotechnol Biofuels

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Background Sugar alcohols are widely used as low-calorie sweeteners in the food and pharmaceutical industries. They can also be transformed into platform chemicals. Yarrowia lipolytica, an oleaginous yeast, is a promising host for producing many sugar alcohols. In this work, we tested whether heterologous expression of a recently identified sugar alcohol phosphatase (PYP) from Saccharomyces cerevisiae would increase sugar alcohol production in Y. lipolytica. Results Y. lipolytica was found natively to produce erythritol, mannitol, and arabitol during growth on glucose, fructose, mannose, and glycerol. Osmotic stress is known to increase sugar alcohol production, and was found to significantly increase erythritol production during growth on glycerol. To better understand erythritol production from glycerol, since it was the most promising sugar alcohol, we measured the expression of key genes and intracellular metabolites. Osmotic stress increased the expression of several key genes in the glycerol catabolic pathway and the pentose phosphate pathway. Analysis of intracellular metabolites revealed that amino acids, sugar alcohols, and polyamines are produced at higher levels in response to osmotic stress. Heterologous overexpression of the sugar alcohol phosphatase increased erythritol production and glycerol utilization in Y. lipolytica. We further increased erythritol production by increasing the expression of native glycerol kinase (GK), and transketolase (TKL). This strain was able to produce 27.5 ± 0.7 g/L erythritol from glycerol during batch growth and 58.8 ± 1.68 g/L erythritol during fed-batch growth in shake-flasks experiments. In addition, the glycerol utilization was increased by 2.5-fold. We were also able to demonstrate that this strain efficiently produces erythritol from crude glycerol, a major byproduct of the biodiesel production. Conclusions We demonstrated the application of a promising enzyme for increasing erythritol production in Y. lipolytica. We were further able to boost production by combining the expression of this enzyme with other approaches known to increase erythritol production in Y. lipolytica. This suggest that this new enzyme provides an orthogonal route for boosting production and can be stacked with existing designs known to increase sugar alcohol production in yeast such as Y. lipolytica. Collectively, this work establishes a new route for increasing sugar alcohol production and further develops Y. lipolytica as a promising host for erythritol production from cheap substrates such as glycerol.

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Production of 1,3-propanediol from glycerol via fermentation by Saccharomyces cerevisiae

Cited by 41 publications

References 84 publications

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production

Fermentation of cellulose pyrolysis oil by a Clostridial bacterium

Metabolic engineering of the oleaginous yeast Yarrowia lipolytica PO1f for production of erythritol from glycerol

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