Production, fractionation, characterization of extracellular polysaccharide from a newly isolated Trametes gibbosa and its hypoglycemic activity

Influence of selenium yeast on the growth, selenium uptake and mineral composition of Coriolus versicolor mushroom

“…Since it is not possible to interrupt the evolution of microorganisms, scientists are forced to search for new antimicrobial substances from various sources [23,24]. The previous research confirmed antioxidative and antibacterial activities of intracellular and extracellular polysaccharides and polysaccharopeptides of Coriolus versicolor medicinal mushroom [6,25,26]. A recently published work showed significant differences in the antibacterial activity of the methanol extract of selenized C. versicolor when different sources of selenium (oganic selenourea and inorganic sodium selenite) were applied during cultivation [15].…”

Structural diversity requires individual optimization of ethanol concentration in polysaccharide precipitation

“…Several challenges exist in this research field: crystal structure determination, quality control, in vivo detection, and molecular target determination [2][3][4][5]. However, before these challenges can be effectively tackled, a more fundamental problem must be addressed, namely, accurate, consistent sample preparation [6][7][8].…”

Principles and application of laser light scattering (LLS) in characterization of the spatial configuration of microbial products in aqueous solution

“…The extracellular polysaccharides can be further purified from the SMP or EPS by removing the proteins and nucleic acids. This is typically achieved by re-dissolving the SMP or EPS pellet in water, in which they are treated with DNAase and RNAase for several hours, then extracting with organic solvents, such as chloroform [26], and Sevag reagent (1:4 n-butanol:chloroform, v/v) [29,30]. The components remaining in the aqueous phase are subsequently harvested, dialyzed, and lyophilized.…”