Production, extraction and purification of phycobiliproteins from Nostoc sp.

Reis, Alberto; Candeias‐Mendes, Ana; Lobo-Fernandes, H.; Empis, José; Novais, J. M.

doi:10.1016/s0960-8524(98)00064-9

Cited by 73 publications

(42 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Due to their limited distribution, these pigments are rather expensive, and obtaining them as pure compounds is a potentially attractive endeavor. 11 As the culture of algae and cyanobacteria is eco-friendly and renewable, there is an increasing tendency to use them as a source of natural colors. Many authors have reported the production of C-PC from Spirulina platensis.…”

Section: Introductionmentioning

confidence: 99%

“…Protein purification techniques use, such as, ammonium sulfate precipitation is of great value, since it can be applied on a large-scale and requires simple equipment and is simple and cheap to carry out. 11 Moreover, for recovering C-phycocyanin biological activity, it is usually excellent after precipitation and its dissolution is easy. There is no standard procedure to purify C-phycocyanin using ammonium sulfate precipitation, therefore it is interesting to study and establish this procedure to reach the maximum possible purity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis

Silva

Kuhn

Moraes

et al. 2009

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

C-ficocianina é um pigmento natural azul que pode ser utilizado como aditivo alimentar e na indústria farmacêutica. Esse trabalho visou obter C-ficocianina com aumento na pureza e sem perdas na recuperação, empregando a técnica da precipitação. O extrato bruto de C-ficocianina foi utilizado nos ensaios. A otimização da purificação foi feita através de um planejamento experimental, no qual avaliou-se a concentração de sulfato de amônio, o volume e o pH de tampão de re-suspensão. Subseqüentemente, um estudo da purificação utilizando o fracionamento com sulfato de amônio foi realizado nas melhores condições encontradas no planejamento experimental. As condições mais favoráveis para a purificação foram obtidas quando foi utilizado fracionamento com sulfato de amônio na faixa 0-20%/20-50%, relação volume de re-suspensão/volume inicial de 0,52 e tampão pH 7,0. Nessas condições, em um único passo de purificação, a pureza aumentou 70% comparado ao extrato inicial e a recuperação foi de 83,8%.C-phycocyanin is a natural blue dye that has been used as an additive in food and can be used to produce medications. The major goal of the present study was to obtain C-phycocyanin under the best operational conditions for high C-phycocyanin recovery and purity using the precipitation technique. Crude C-phycocyanin from Spirulina platensis was used. Optimization of the purification was carried out using experimental design. The effect of ammonium sulfate concentration, volume and of pH for resuspension were evaluated. Subsequently an ammonium sulfate fractionation study was carried out using the most suitable conditions found in the experimental design. The best purification condition was ammonium sulfate fractionation at 0-20%/20-50%, in relation to a resuspension volume/initial volume of 0.52 in a 7.0 pH buffer. Under these conditions, in an one-step purification only, the purity increased 70% compared to the initial extract, with an 83.8% recovery.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis

Silva

Kuhn

Moraes

et al. 2009

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…They are divided into three classes based on their adsorption properties: phycoerythrins, phycocyanins, and allophycocyanins. The main function of phycobiliproteins is to participate in the photosynthetic process together with chlorophyll-a and carotenoids [21,25].…”

Section: Introductionmentioning

confidence: 99%

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Silveira

Quines

Burkert

et al. 2008

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

This paper presents the evaluation of some important parameters for the purification of phycocyanin using ion exchange chromatography. The influences of pH and temperature on the equilibrium partition coefficient were investigated to establish the best conditions for phycocyanin adsorption. The equilibrium isotherm for the phycocyanin-resin system was also determined. The separation of phycocyanin using the Q-Sepharose ion exchange resin was evaluated in terms of the pH and elution volume that improved the increase in purity and recovery. The highest partition coefficients were obtained in the pH range from 7.5 to 8.0 at 25 degrees C. Under these conditions the equilibrium isotherm for phycocyanin adsorption was well described by the Langmuir model, attaining a Q (m) of 22.7 mg/mL and K (d) of 3.1 x 10(-2) mg/mL. The best conditions for phycocyanin purification using the ion exchange column were at pH 7.5 with an elution volume of 36 mL, obtaining 77.3% recovery and a 3.4-fold increase in purity.

show abstract

“…The solution containing protein with the highest purity (1.35) was then placed at increasing temperature points ranging from 25˚C to 45˚C (5˚C interval between every two temperatures) and still the purity was nearly unchanged. Ultrafiltration was often applied in the later steps of primary protein isolation [33]. We showed in this study that the purities of PE and PC from P. yezoensis in the extracted solutions were increased from 0.9 to 1.4 or 0.5 to 0.7, respectively, when ultrafiltration was utilized.…”

Section: Primary Isolation Of Pbp From P Yezoensismentioning

confidence: 81%

Large scale preparation of phycobiliproteins from <i>Porphyra yezoensis</i> using co-precipitation with ammonium sulfate

Cai¹,

Li²,

Wu³

et al. 2012

View full text Add to dashboard Cite

The purpose of this study was to extract phycobiliproteins (PBP) from Porphyra yezoensis using an optimized procedure and further establish a large scale process for protein production. According to our previous experiences on the extraction of PBP, salting out methods, e.g. ammonium sulfate precipitation, worked more efficiently than isoelectric precipitation, differential centrifugation or ultrafiltration. Thus, we chose ammonium sulfate to coprecipitate PBP in crude solution. After four times of precipitation followed by one time of high speed centrifugation, the maximum purity of crude phycoerythrin and phycocyanin reached 1.94 (A 565 /A 280 ) and 0.85 (A 615 /A 280 ), with a yield of 0.50% and 0.37%, respectively. A total of 0.94 mg phycoerythrin and 0.54 mg phycocyanin with purity of more than 3.2 were obtained from 1 g dried P. yezoensis after additional chromatography. We further scaled up the frozen dried P. yezoensis from 20 g to 400 g, with 1295 mg phycoerythrin and 593 mg phycocyanin obtained.

show abstract

Production, extraction and purification of phycobiliproteins from Nostoc sp.

Cited by 73 publications

References 7 publications

Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis

Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Large scale preparation of phycobiliproteins from <i>Porphyra yezoensis</i> using co-precipitation with ammonium sulfate

Contact Info

Product

Resources

About

Production, extraction and purification of phycobiliproteins from Nostoc sp.

Cited by 73 publications

References 7 publications

Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis

Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis

Separation of phycocyanin from Spirulina platensis using ion exchange chromatography

Large scale preparation of phycobiliproteins from &lt;i&gt;Porphyra yezoensis&lt;/i&gt; using co-precipitation with ammonium sulfate

Contact Info

Product

Resources

About

Large scale preparation of phycobiliproteins from <i>Porphyra yezoensis</i> using co-precipitation with ammonium sulfate