Production effects and bioavailability of N-acetyl-l-methionine in lactating dairy cows

Räisänen, S.E.; Zhu, Xiaoyun; Zhou, Chuanshe; Lage, C.F.A.; Fetter, M. E.; Silvestre, T.; Stefenoni, H.; Wasson, D.E.; Cueva, S.F.; Eun, Jong‐Su; Moon, Jungyoon; Park, J.S.; Hristov, A.N.

doi:10.3168/jds.2021-20540

Cited by 5 publications

(8 citation statements)

References 39 publications

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“…Aside from plasma Met, NALM dosing did not affect concentrations of BCAA in the recently reported studies by Liang et al (2019) and Räisänen et al (2022). Appuhamy et al (2011) reported that jugular infusion of a combination of L-Lys and L-Met led to a 17% reduction in plasma Ile concentration compared with a control treatment.…”

Section: Discussionmentioning

confidence: 73%

“…Unlike rumen dosing, an abomasal pulse dose of L‐Met or NALM (after deacetylation) is readily available for absorption, and plasma Met concentrations in the current study increased over the first 3 h after abomasal infusion of NALM (Figure 1). Räisänen et al (2022) reported that plasma Met concentration peaked around 1 h after abomasal dosing with L‐Met, whereas the peak plasma Met was reached around 2 to 4 h after abomasal dosing lactating Holstein cows with two NALM doses at 14.4 and 28.8 g/cow/day. Daabees et al (1984) noted when equimolar amounts of L‐Met and NALM were supplemented to young pigs, the portal and venal caval peak plasma Met concentrations were lower for NALM compared with L‐Met.…”

Section: Discussionmentioning

confidence: 99%

“…Plasma for AA analyses was composited by treatment and hour for each period. Plasma for Met and AA concentrations were analyzed by CJ CheilJedang using HPLC (Agilent 1260 Infinity II LC System) as described by Räisänen et al (2022). In addition, the plasma samples were analyzed for NALM by CJ CheilJedang according to Räisänen et al (2022).…”

Section: Aa and Plasma Nalm Analysesmentioning

confidence: 99%

“…Plasma for Met and AA concentrations were analyzed by CJ CheilJedang using HPLC (Agilent 1260 Infinity II LC System) as described by Räisänen et al (2022). In addition, the plasma samples were analyzed for NALM by CJ CheilJedang according to Räisänen et al (2022). Briefly, the samples were centrifuged at 20,000Âg for 15 min at 4 C and then added on an Amicon Ultra filter (0.5 ml, 3000 molecular weight cutoff; Millipore Sigma, Burlington, MA, USA).…”

Section: Aa and Plasma Nalm Analysesmentioning

confidence: 99%

“…Several non‐ruminant animal studies concluded that the bioavailability of L‐Met and NALM was similar based on feeding studies measuring growth rates and weight gain (Boggs et al, 1975; Friedman & Gumbmann, 1987; Young Jenkins et al, 1978). In ruminants, the relative bioavailability of abomasally infused NALM was recently estimated as 50% when dosed at 14.4 g/day and 24% when dosed at 28.8 g/day using the area under the curve technique (Räisänen et al, 2022). To date, however, there is limited research reported on the effects of supplementing NALM at different dose rates, consisting of a normal (30 g/day) and high (60 g/day) rate to lactating dairy cows on plasma Met concentration and residue analysis in plasma, milk, liver, and muscle.…”

Section: Introductionmentioning

confidence: 99%

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Plasma methionine appearance and residual potential of supplemented N‐acetyl‐L‐methionine through ruminal or abomasal infusion in dairy cows

Hall

Eun

2022

Animal Science Journal

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The present study investigated the plasma methionine (Met) and residual potential of N-acetyl-L-Met (NALM) in lactating dairy cows. Six cows (75 AE 20.1 days-in-milk) were used in a replicated 3 Â 3 Latin square design. Within each square, cows were randomly assigned to a sequence of three dietary treatments during each of the three 13-day periods (10 days of treatment adaptation and 3 days of data collection and sampling). The three dietary treatments are as follows: basal diet without NALM (control); control diet with 30 g/day of NALM by rumen placement (30NALM), and control diet with 60 g/day of NALM by rumen placement (60NALM). Rumen NALM dosing led to a linear increase in plasma Met concentration. Abomasal infusion with NALM resulted in both linear and quadratic increases in plasma Met concentration.No NALM was detected in milk, liver, plasma, and muscle samples after rumen placement or abomasal infusion. Supplementation of NALM did not affect dry matter intake and milk yield. The absence of plasma NALM and increases in plasma Met concentration for both ruminal and abomasal NALM dosing suggest that NALM supplemented by either rumen placement or abomasal infusion to lactating dairy cows is deacetylated before entering the central circulation.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Aa and Plasma Nalm Analysesmentioning

confidence: 99%

Section: Aa and Plasma Nalm Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plasma methionine appearance and residual potential of supplemented N‐acetyl‐L‐methionine through ruminal or abomasal infusion in dairy cows

Hall

Eun

2022

Animal Science Journal

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Resolution of N‐acetyl‐DL‐methionine methyl ester by the lipase from Brucella thiophenivorans

Li,

Yang

et al. 2024

Chirality

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In this study, lipase‐catalyzed resolution of N‐acetyl‐DL‐methionine methyl ester (N‐Ac‐DL‐MetOMe) was evaluated. A lipase from Brucella thiophenivorans was prone to exhibit high activity and excellent enantioselectivity toward N‐Ac‐DL‐MetOMe to produce the key chiral intermediate N‐acetyl‐L‐methionine methyl ester (N‐Ac‐L‐MetOMe). The results showed that the enzymatic reaction was carried out in 100 g/L racemic substrate for 2 h, the conversion reached 51.3%, the enantiomeric excess value N‐Ac‐L‐MetOMe exceeded 99%, and the enantiomeric ratio value >200. Therefore, the lipase from B. thiophenivorans has potential prospects for the resolution of N‐Ac‐DL‐MetOMe to produce the important intermediate N‐Ac‐L‐MetOMe.

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Production of rumen- and gastrointestinal-resistant nanoparticles to deliver lysine to dairy cows

Albuquerque,

Neves,

Van Dorpe

et al. 2023

Sci Rep

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Supplementing diets with rumen-protected lysine is a common strategy to meet the nutritional needs of high-producing dairy cows. This work addressed two separate but crucial issues: the lysine protection degree across the entire digestive tract as well as the production scalability of the proposed delivery systems. This was achieved by evaluating, in vitro or ex vivo, previously developed rumen-resistant lipid nanoparticles regarding their stability in the digestive tract and in the bloodstream of the dairy cow as well as how their production could be scaled-up. Results showed that the developed nanoparticles were able to resist digestion along the digestive tract but were degraded in the blood over 24 h. Thus, releasing their content to be used by the animal. In vitro viability assays were also performed, with the nanoparticles being found not to be inherently toxic when using nanoparticle concentrations up to 1 mg/mL. Results showed that neither the purity of the used lipids nor the production method significantly altered the nanoparticles’ properties or their ruminal resistance. Furthermore, the shelf-life of these nanoparticles was assessed, and they were found to retain their properties and remain usable after at least 1 month of storage. Moreover, a pilot-scale production allowed the production of nanoparticles with similar properties to the previous ones made using standard methods. To summarize, the proposed rumen-resistant nanoparticles presented potential as orally ingested lysine delivery systems for dairy cattle supplementation, being capable of a large-scale production using cheaper components while maintaining their properties and without any efficiency loss. It should however be noted that these results were obtained mainly in vitro and further in vivo bioavailability and production experiments are needed before this technology can be confirmed as a viable way of delivering lysine to dairy cows.

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Production effects and bioavailability of N-acetyl-l-methionine in lactating dairy cows

Cited by 5 publications

References 39 publications

Plasma methionine appearance and residual potential of supplemented N‐acetyl‐L‐methionine through ruminal or abomasal infusion in dairy cows

Plasma methionine appearance and residual potential of supplemented N‐acetyl‐L‐methionine through ruminal or abomasal infusion in dairy cows

Resolution of N‐acetyl‐DL‐methionine methyl ester by the lipase from Brucella thiophenivorans

Production of rumen- and gastrointestinal-resistant nanoparticles to deliver lysine to dairy cows

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