Production, chemical composition and prostaglandin F-2  content of uterine fluid in pregnant sheep

Harrison, F. A.; Heap, R. B.; Poyser, N.L.

doi:10.1530/jrf.0.0480061

Cited by 38 publications

(5 citation statements)

References 9 publications

(9 reference statements)

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“…However, Ca 2+ concentration was modulated during different stages of the estrous cycle in bovines, with highest activity in early‐luteal and follicular stage ULFs (Schultz et al, 1971). Similarly, a four‐ to fivefold increased Ca 2+ concentration was found in 115‐day pregnant sheep ULF as compared to that of non‐pregnant (Harrison et al, 1976). It is possible that Ca 2+ level of buffalo ULF is modulated during the estrous cycle and involved in regulating the activity of these gelatinases/MMPs.…”

Section: Discussionmentioning

confidence: 85%

Dynamic in vivo changes in the activities of gelatinases, matrix metalloproteinases (MMPs), and tissue inhibitor of metalloproteinases (TIMPs) in buffalo (Bubalus bubalis) uterine luminal fluid during estrous cycle and early pregnancy

Roy

Ghosh

2010

Molecular Reproduction Devel

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In ruminants, the phenomenon of endometrial tissue remodeling during the estrous cycle and early pregnancy is not fully understood. In this report, the occurrence of tissue remodeling, if any, in buffalo endometrium was studied by detecting gelatinases, matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs); the key regulators of tissue remodeling, in uterine luminal fluids (ULF) of cycling and early pregnant (approx. 43-65 days) buffaloes. Each stage of the estrous cycle and pregnant ULF demonstrated a unique profile of gelatinase activities compared to serum/follicular fluid, with a major gelatinase band of 60 kDa with highest activity in early-luteal stage. In addition to a 32 kDa uterus-specific gelatinase band detected in both non-pregnant and pregnant ULFs, the pregnant ULF displayed three new gelatinase bands of 86, 78, and 57 kDa. Western blot technique confirmed the presence of MMP-2 (54 kDa), MMP-9 (76/73 kDa), TIMP-1 (32 kDa), TIMP-2(20 kDa), and two molecular weight forms (31 and 22 kDa) of TIMP-3 in buffalo ULF with varying band intensities. Highest MMP-2 and MMP-9 activities were observed in follicular and early-luteal stage ULFs, respectively. Highest TIMP-1 activity was observed in early-luteal ULF. Interestingly, TIMP-2 activity was only detected in mid-luteal, late-luteal, and follicular stage ULFs with significantly increasing intensities. Highest activities of 31 and 22 kDa TIMP-3 were associated with late-luteal and early-luteal stage ULFs, respectively. The varied activities of MMPs and TIMPs in buffalo ULF during the estrous cycle and early pregnancy might be a reflection of dynamic structural remodeling of the endometrium and/or developing conceptus.

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Section: Discussionmentioning

confidence: 85%

Dynamic in vivo changes in the activities of gelatinases, matrix metalloproteinases (MMPs), and tissue inhibitor of metalloproteinases (TIMPs) in buffalo (Bubalus bubalis) uterine luminal fluid during estrous cycle and early pregnancy

Roy

Ghosh

2010

Molecular Reproduction Devel

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“…PLA-2 is then activated by the Ca2 + to release arachidonic acid, which is then metabolized predominantly into PGF-2a. Support for this proposal is provided by the previous finding that uterine fluid in sheep contains high concentrations of PGF-2a, calcium and protein (Harrison, Heap & Poyser, 1976). In early pregnancy, the conceptuses must prevent this proposed process in some way.…”

Section: Discussionmentioning

confidence: 88%

Prostaglandin production by the early pregnant guineapig uterus in relation to implantation and luteal maintenance, and the effect of oestradiol

Poyser¹

1984

Reproduction

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The outputs of prostaglandin (PG) F-2 alpha and PGE-2, but not of 6-oxo-PGF-1 alpha, from the guinea-pig uterus were significantly lower on Days 7 and 15 of pregnancy than on the corresponding days of the cycle. Uterine PGF-2 alpha output increased 28-fold between Days 7 and 15 of the cycle but only 4- to 5-fold between these same days of pregnancy. Uterine PGE-2 and 6-oxo-PGF-1 alpha outputs increased 2- to 3-fold between Days 7 and 15 of the cycle and of pregnancy. Endometrial PGF-2 alpha synthesizing capacity was 60-70% lower on Days 7 and 15 of pregnancy than on the corresponding days of the cycle, although it increased 2-fold and 2.5-fold between these days of pregnancy and of the cycle, respectively. Endometrial PGE-2 and 6-oxo-PGF-1 alpha synthesizing capacities showed no significant variation amongst Days 7 and 15 of the cycle and of pregnancy, except that endometrial PGE-2 synthesizing capacity was lower on Day 7 of the cycle. Oestradiol treatment (10 micrograms s.c. daily from Days 10 to 14 of pregnancy) did not affect plasma progesterone concentrations, uterine 6-oxo-PGF-1 alpha output, and endometrial PGF-2 alpha, PGE-2 and 6-oxo-PGF-1 alpha synthesizing capacities in 9/12 guinea-pigs when examined on Day 15. Uterine PGF-2 alpha and PGE-2 outputs increased 3- and 1.5-fold, respectively, in these guinea-pigs, but were still much lower than the outputs from the Day-15 non-pregnant uterus. The pregnancies appeared unaffected in these oestradiol-treated guinea-pigs. In the other 3 oestradiol-treated animals, uterine PGF-2 alpha output was 20- to 30-fold higher than in untreated, pregnant guinea-pigs on Day 15, and 2- to 3-fold higher than in Day-15 non-pregnant guinea-pigs. Uterine PGE-2 and 6-oxo-PGF-1 alpha outputs also tended to be higher in these treated guinea-pigs. In these 3 guinea-pigs, endometrial PGF-2 alpha, PGE-2 and 6-oxo-PGF-1 alpha synthesizing capacities were 4.0-, 3.4- and 2.5-fold higher, respectively, than in untreated, pregnant guinea-pigs on Day 15, and tended to be higher than in Day-15 non-pregnant guinea-pigs. Plasma progesterone concentrations were much lower in these 3 animals than in the other 9 treated with oestradiol, and also much lower than in untreated, pregnant guinea-pigs on Day 15.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…The fact that the response is blocked argues for a direct action of progesterone on the myometrium, although this might not be so if transfer of PGs from the lumen to the myometrium was impaired by progesterone. This possibility would seem remote because synthesis of PGs by the endometrium is enhanced by progesterone (Louis, Parry, Robinson, Thorburn & Challis, 1977) and Harrison, Heap & Poyser (1976) have shown that both uterine fluid and uterine vein blood contain substantial amounts of PGs (i.e. synthesis and re¬ lease are unimpaired) in ewes with high plasma progesterone levels.…”

Section: Discussionmentioning

confidence: 99%

Demonstration that progesterone 'blocks' uterine activity in the ewe in vivo by a direct action on the myometrium

Lye¹,

Porter²

1978

Reproduction

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Intrauterine pressure was monitored in vivo in oestrogen-treated ovariectomized ewes before, during and after treatment with progesterone (50 mg s.c./day for 3 days). Progesterone reversibly reduced the frequency and amplitude of myometrial activity and abolished uterine reactivity to oxytocin (i.v.) and PGF-2alpha (intrauterine infusion). The rate of rise of intrauterine pressure during active pressure cycles was significantly reduced. These results confirm that the action of progesterone on the ovine myometrium is comparable to the classic progesterone 'block'. The intrauterine infusion of PGF-2alpha (10 microgram/min), which elicited a marked mechanical response in the control animals, failed to stimulate the progesterone-'blocked' uterus, suggesting that the inhibition produced by progesterone is due to a direct action of the hormone on the uterine muscle and not to an indirect mechanism operating through endometrial prostaglandin output.

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Production, chemical composition and prostaglandin F-2 content of uterine fluid in pregnant sheep

Cited by 38 publications

References 9 publications

Dynamic in vivo changes in the activities of gelatinases, matrix metalloproteinases (MMPs), and tissue inhibitor of metalloproteinases (TIMPs) in buffalo (Bubalus bubalis) uterine luminal fluid during estrous cycle and early pregnancy

Dynamic in vivo changes in the activities of gelatinases, matrix metalloproteinases (MMPs), and tissue inhibitor of metalloproteinases (TIMPs) in buffalo (Bubalus bubalis) uterine luminal fluid during estrous cycle and early pregnancy

Prostaglandin production by the early pregnant guineapig uterus in relation to implantation and luteal maintenance, and the effect of oestradiol

Demonstration that progesterone 'blocks' uterine activity in the ewe in vivo by a direct action on the myometrium

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