Production, Characterization, and Stability of Orange or Eucalyptus Essential Oil/β‐Cyclodextrin Inclusion Complex

Abstract

The aim of this study was to produce and characterize inclusion complexes (IC) between β-cyclodextrin (β-CD) and orange essential oil (OEO) or eucalyptus essential oil (EEO), and to compare these with their pure compounds and physical mixtures. The samples were evaluated by chemical composition, morphology, thermal stability, and volatile compounds by static headspace-gas chromatography (SH-GC). Comparing the free essential oil and physical mixture with the inclusion complex, of both essential oils (OEO and EEO), it was observed differences occurred in the chemical composition, thermal stability, and morphology. These differences show that there was the formation of the inclusion complex and demonstrate the necessity of the precipitation method used to guarantee the interaction between β-CD and essential oils. The slow loss of the volatile compounds from both essential oils, when complexed with β-CD, showed a higher stability when compared with their physical mixtures and free essential oils. Therefore, the results showed that the chemical composition, molecular size, and structure of the essential oils influence the characteristics of the inclusion complexes. The application of the β-CD in the formation of inclusion complexes with essential oils can expand the potential applications in foods.

Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β‐Cyclodextrin: Characterization, Stability, and Antioxidant Activity

“…The results of Kringel et al. () also suggested that CD inclusion complexes could efficiently and stably encapsulate guest molecules even at high temperatures due to the ability of the β‐CD to efficiently retain essential oils to prevent their evaporation.…”

“…Preparation of the inclusion complex: EEO inclusion complex (β‐CD‐EEO) was performed according to a method described in the published literature (Kringel et al., ; Xiang, Zhang, Li, & Li, ). β‐CD (3.5 g) was dissolved in 100 mL of distilled water maintained at 40 °C in a constant temperature heating stirrer, and then 2.0 mL of an essential oil and ethanol mixture (1:1 V/V) was slowly added to the β‐CD solution.…”

“…One way to overcome these problems is to include drugs as guest molecules into cyclodextrins (CDs). Currently, essential oil has been reported to have good stability and biological activity after forming an inclusion complex (Babaoglu, Bayrak, Ozdemir, & Ozgun, ; Kringel et al., ).…”

Free and encapsulated orange essential oil into a β‐cyclodextrin inclusion complex and zein to delay fungal spoilage in cakes

“…The peels of Valencia oranges were frozen and crushed to increase the extraction yield. The essential oil was obtained by a hydro‐distillation technique using a Clevenger apparatus in accordance with the method described by Kringel et al ().…”

“…The β‐CD/OEO inclusion complex was prepared by the precipitation method as described by Kringel et al ().…”