Production, biological activity, and structure of recombinant basic fibroblast growth factor and an analog with cysteine replaced by serine.

Fox, Gary M.; Schiffer, Steffen; Rohde, Michael F.; Tsai, Larry B.; Banks, Allen R.; Arakawa, Tsutomu

doi:10.1016/s0021-9258(19)81379-0

Cited by 98 publications

(13 citation statements)

References 44 publications

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“…~NGF and EGF were prepared by the method of Mobley et al (1976) and Savage and Cohen (1972), respectively, bFGF used in all experiments was an analog with all haif-cystine residues replaced by serines (Fox et al, 1988) (kindly provided by Dr. Michael Fox, Amgen, Inc., Thousand Oaks, CA). PMA, 8-Br-cAMP, and sodium orthovanadate were from Sigma Chemical Co. (St. Louis, Mo.).…”

Section: Methodsmentioning

confidence: 99%

Effect of nerve growth factor and fibroblast growth factor on PC12 cells: inhibition by orthovanadate.

Bradshaw

1993

The Journal of Cell Biology

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Abstract. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, causes increased levels of tyrosine phosphorylation and blocks, at noncytotoxic concentrations, the differentiative response of rat pheochromocytoma (PC12) cells to/3-nerve growth factor (/3NGF) and basic fibroblast growth factor (bFGF) in a reversible manner. It also prevents growth factorinduced neurite proliferation in primed cells and causes the retraction of previously formed neurites, even in the presence of/3NGF or bFGE It is equally effective in blocking neurite proliferation by 8-Br-cAMP. Zinc chloride and ammonium molybdate, two other inhibitors of tyrosine phosphatases, also cause parallel decreases in neurite proliferation. Orthovanadate generally reduces the transcription of immediate early response genes (TIS 8 and c-fos) and secondary response genes (ornithine decarboxylase (ODC), acetylcholinesterase (ACHE) and SCG 10) induced by/3NGF, bFGF, EGF, and PMA, albeit in a variable fashion.There was no observed effect on the kinetics of expression as judged by TIS 8 induction by/3NGF and protein kinase C (PKC) downregulation did not change the levels of inhibition by orthovanadate seen in control cells. Orthovanadate does not affect the production of diacylglycerol induced by/3NGF or bFGE These observations are consistent with the view that growth factor stimulation of differentiation in PC12 cells involves at least one other PKC independent pathway, and that cAMP and PMA (and their active analogs) activate tyrosine kinases (albeit probably secondarily), which are at least partially responsible for their actions. Although the exact site(s) of action of orthovanadate that lead to the inhibition of growth factor-induced neurite proliferation are unknown, the results presented suggest that it prolongs tyrosine phosphorylations by nonreceptor tyrosine kinases that act downstream from the receptor kinases.

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Section: Methodsmentioning

confidence: 99%

Effect of nerve growth factor and fibroblast growth factor on PC12 cells: inhibition by orthovanadate.

Bradshaw

1993

The Journal of Cell Biology

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“…Two are conserved among all members of the FGF family (71) and may be important in determining the tertiary structure of bFGF through intramolecular disulfide bonds. The two remaining cysteines are not essential for biological activity (19). In recombinant bFGF produced in Escherichia cold, these two cysteines appear to remain reduced (19).…”

Section: Historymentioning

confidence: 99%

“…The two remaining cysteines are not essential for biological activity (19). In recombinant bFGF produced in Escherichia cold, these two cysteines appear to remain reduced (19). However, the two nonessential cysteines in natural bFGF may be involved in disulfide bonds with free cysteines since the initial amino acid analysis of bovine pituitary bFGF yielded six cysteines (14).…”

Section: Historymentioning

confidence: 99%

Recent developments in the cell biology of basic fibroblast growth factor.

Rifkin

Moscatelli

1989

The Journal of Cell Biology

989

361

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“…FGF2 is also called the basic fibroblast growth factor (bFGF). In 1974, it was first isolated from the bovine pituitary (Lemmon & Bradshaw, 1975), and in 1988, the first human recombinant FGF2 was reported (Fox et al, 1988). The molecular weight of its secreted isoform is 18 kDa.…”

Section: Discussionmentioning

confidence: 99%

Identification of YAP1 as a novel downstream effector of the FGF2/STAT3 pathway in the pathogenesis of renal tubulointerstitial fibrosis

Zhang

et al. 2021

Journal Cellular Physiology

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Chronic kidney disease is a global health problem and eventually develops into an end-stage renal disease (ESRD). It is now widely believed that renal tubulointerstitial fibrosis (TIF) plays an important role in the progression of ESRD. Renal tubular epithelial-mesenchymal transition (EMT) is an important cause of TIF. Studies have shown that FGF2 is highly expressed in fibrotic renal tissue, although the mechanism remains unclear. We found that FGF2 can activate STAT3 and induce EMT in renal tubular epithelial cells. STAT3, an important transcription factor, was predicted by the JASPAR biological database to bind to the promoter region of YAP1. In this study, STAT3 was shown to promote the expression of the downstream target gene YAP1 through transcription, promote EMT of renal tubular epithelial cells, and mediate the occurrence of renal TIF. This study provides a theoretical basis for the involvement of the FGF2/STAT3/YAP1 signaling pathway in the process of renal interstitial fibrosis and provides a potential target for the treatment of renal fibrosis.epithelial-mesenchymal transition (EMT), fibroblast growth factor 2 (FGF2), renal tubulointerstitial fibrosis (TIF), signal transducer and activator of transcription 3 (STAT3), Yes-associated protein 1 (YAP1)

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Production, biological activity, and structure of recombinant basic fibroblast growth factor and an analog with cysteine replaced by serine.

Cited by 98 publications

References 44 publications

Effect of nerve growth factor and fibroblast growth factor on PC12 cells: inhibition by orthovanadate.

Effect of nerve growth factor and fibroblast growth factor on PC12 cells: inhibition by orthovanadate.

Recent developments in the cell biology of basic fibroblast growth factor.

Identification of YAP1 as a novel downstream effector of the FGF2/STAT3 pathway in the pathogenesis of renal tubulointerstitial fibrosis

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