2004
DOI: 10.1016/j.gene.2004.06.058
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Production and secretion of biologically active recombinant canine growth hormone by Pichia pastoris

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Cited by 18 publications
(17 citation statements)
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“…It is noteworthy that all the fermentations reported so far for hGH and GH from other sources were performed with complex medium for the Pichia expression system [1,5,20]. In our present study, however, using BSM, we could successfully achieve substantially higher yields in 24 h. Two reasons could be attributed for such an observation, one being the high‐copy‐number integration of hGH gene and the other the high secretion efficiency.…”
Section: Discussionmentioning
confidence: 52%
“…It is noteworthy that all the fermentations reported so far for hGH and GH from other sources were performed with complex medium for the Pichia expression system [1,5,20]. In our present study, however, using BSM, we could successfully achieve substantially higher yields in 24 h. Two reasons could be attributed for such an observation, one being the high‐copy‐number integration of hGH gene and the other the high secretion efficiency.…”
Section: Discussionmentioning
confidence: 52%
“…We previously used this type of construction in P. pastoris for the production and secretion of 22 kDa 10 and 20 kDa (unpublished results) human growth hormone, a bacterial phytase 16 and a laccase from Trametes (unpublished results). In addition, other authors have successfully used this construction, 17 and an inefficient removal of the Glu-Ala repeats from a secreted recombinant protein produced in P. pastoris was described. [18][19][20] The transcription process of the shrimp trypsinogen II cDNA expression cassette was demonstrated by RT-PCR assays.…”
Section: Discussionmentioning
confidence: 99%
“…Various reports claim that higher recombinant protein production levels are obtained using MutS than when using Mut+ (Ascacio‐Martínez & Barrera‐Saldaña, ; Bredell, Smith, Görgens, & van Zyl, ; Cos et al, ; Krainer et al, ; Orman, Çalık, & Özdamar, ; Pla et al, ). However, the relatively few reports directly comparing Mut+ with MutS usually only focus on the final product yields and sometimes production rates and less on the reasons behind the observed differences.…”
Section: Introductionmentioning
confidence: 99%