Production and secretion of an interleukin‐l‐like factor is stage‐dependent and correlates with spermatogonial DNA synthesis in the rat seminiferous epithelium

Söder, Olof; Syed, Viqar; Callard, Gloria V.; Toppari, Jorma; Pöllänen, Pasi; Parvinen, Martti; Fröysa, Berit; Ritzén, E. M.

doi:10.1111/j.1365-2605.1991.tb01084.x

Cited by 75 publications

(38 citation statements)

References 37 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pituitary adenylate cyclase-activating polypeptide (PACAP) is most intensely expressed in spermatids steps 3 -12 but not in maturation phase spermatids, its receptor in spermatids step 7 -12, and expression of the PACAP gene occurs in spermatogonia and primary spermatocytes [14,15]. Stage-specific expression was recognized also for other molecules as for IGF-I, activin, inhibin, NGFβ, transcriptional factors, interleukins [4][5][6]8,10,11]. We also believe that expression of GHSR-1a isoforms illustrates conditions for a response of spermatogenic cells to ghrelin whithin the seminiferous epithelium and the process in which spermatozoa are furnished receiving receptor to respond, for instance, to seminal plasma ghrelin or to the ghrelin of female reproductive tract fluid available in the course of movement and capacitation of spermatozoa or acrosome reaction and fertilization.…”

Section: Discussionmentioning

confidence: 99%

“…The local regulatory mechanism has been defined during last two decades and the list of its messengers, the local signalling molecules, has been growing with years. The list includes growth factors, polypeptides of hormonal and paracrine nature, transcriptional modulators, interleukins and receptors of these molecules, all of them being the mediators in a cross talk between somatic and germ cells [4][5][6][7][8][9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Lukaszyk

Rafinska²,

P³

et al. 2010

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Abstract:The results of presented study demonstrate expression of ghrelin, its functional receptor GHSR-1a and their genes in spermatogenic cells of rat testis suggesting their functioning within seminiferous epithelium. The immunohistochemical and hybrydocytochemical expression, of proteins and transcripts, was estimated taking into account the cycle of seminiferous epithelium and phases of spermatogenesis. Both transcripts and ghrelin was found to show nuclear expression and scarcely cytoplasmic. Expression of genes for ghrelin and GHSR-1a was shown in early spermatocytes and round spermatids representing transcriptional phases of meiosis and spermiogenesis. Ghrelin was evidenced to show nuclear expression in two stage-specific windows, in late spermatogonia, in spermatocytes up to early pachytenes, and again in spermatids of acrosome and early maturation phase of spermiogenesis. In late pachytenes, secondary spermatocytes, round spermatids, maturing spermatids and spermatozoa the reaction is lacking. With two types of antibodies against the GHSR-1a used the two different patterns of immunostaining was evidenced suggesting two isoforms of GHSR-1a. The first evidenced GHSR1a in cytoplasm of spermatocytes, cell membrane and acrosomes of spermatids, Sertoli cell processes and heads of spermatozoa. With second type of antibodies the immunostaining marks all steps of evolution of acrosome in spermatids. It is believed that site of ghrelin expression in seminiferous epithelium may indicate its role in local regulations, not excepting the intracellular signalling. Immunostaining pattern for GHSR-1a seems to suggest both its participation in the cross-talk among the cells and also process of furnishing gametes with GHSR-1a for its response to ghrelin in seminal plasma or female reproductive tract.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Lukaszyk

Rafinska²,

P³

et al. 2010

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…Quality and quantity of isolated nucleic acids were next evaluated. Target RNA (3 mg) was reversely transcribed with Super Script (Invitrogen) reverse transcriptase enzyme and oligo(dT) 15 primer.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

et al. 2007

View full text Add to dashboard Cite

Interleukin-1 (IL-1) is a pleiotropic cytokine that may play a role in contributing to the specific immune environment of mammalian testis and in regulating cell differentiation. We have determined the transcription activity of the IL-1 gene family (using real-time polymerase chain reaction (PCR)) in two main functional testicular compartments (interstitial and intratubular ones), and in tissue homogenates obtained from patients with fertility disorders (spermatogenic arrest and testicular tumors). We observed the prominent expression of gene coding for IL-1 receptor antagonist (IL-1RA) in a purified fraction of gametogenic cells (normal gonad). Caspase-1 (ICE -IL-1b-converting enzyme) was highly expressed (on mRNA level) in interstitial compartments as well in testicular tumors (immune enhancement?). In addition we found, that the activity of IL-1RA gene decreased along spermatogenic alteration in an inversely related manner with IL-1a (from normal gonad through spermatogenic arrest to Sertoli cell only syndrome). Therefore, the quotient value of IL-1a/IL-1RA could potentially serve as the diagnostic molecular probe for spermatogenesis assessment. The precise level of mRNA for IL-1-IL-18 cytokines and their receptors, and specifically of the receptor antagonist in immune privileged gonad, could be one of the main factors responsible for maintaining testicular homeostasis, thus enabling generation of the mature spermatozoa.

show abstract

“…In the rat testis, bioactive IL-1 protein first appears during puberty and in the adult rat the production remains constantly high, although displaying a stage-dependent production (5,6). The function of testicular IL-1␣ is obscure, but some data indicate that it may be involved in the paracrine regulation of germ cell growth and differentiation during spermatogenesis (7,8).…”

mentioning

confidence: 99%

Constitutive Expression of Interleukin-1 Messenger Ribonucleic Acid in Rat Sertoli Cells Is Dependent upon Interaction with Germ Cells

Jonsson

1999

Endocrinology

View full text Add to dashboard Cite

Interleukin-1 (IL-1), a proinflammatory cytokine originally isolated as a product of activated mononuclear phagocytes, consists of two distinct agonist proteins, IL-1␣ and IL-1␤, of which IL-1␤ is the major inducible IL-1 protein produced by macrophages. We show here that mRNA of IL-1␣, but not IL-1␤, is constitutively expressed by the intact rat testis and localize the transcript to Sertoli cells as confirmed by a novel squash technique. The expression is developmentally regulated and appears only after postnatal day 20 in the rat testis, corresponding to onset of puberty. IL-1␣ mRNA shows a stage-dependent expression pattern during the cycle of the seminiferous epithelium. It is low or absent in stage VII, but present in all other stages of the cycle. The same stage-dependent distribution was also observed at the protein level when bioactive IL-1 was measured in extracts of accurately defined one millimeter segments of seminiferous tubules. No IL-1␣ mRNA was detected in adult rat testes after germ cell depletion by fetal irradiation or cytostatic drug treatment. Because stage VII is the only segment of the seminiferous tubules lacking DNA replication, we propose that IL-1␣ is involved in this event during mitosis and meiosis of spermatogenesis and that its expression is dependent upon interactions between Sertoli cells and germ cells.

show abstract

Production and secretion of an interleukin‐l‐like factor is stage‐dependent and correlates with spermatogonial DNA synthesis in the rat seminiferous epithelium

Cited by 75 publications

References 37 publications

Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Immunohistochemical and hybridocytochemical study on ghrelin signalling in the rat seminiferous epithelium.

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

Constitutive Expression of Interleukin-1 Messenger Ribonucleic Acid in Rat Sertoli Cells Is Dependent upon Interaction with Germ Cells

Contact Info

Product

Resources

About