Production and Purification of Xylanase From Indonesian Isolate Bacillus sp. AQ-1 Grown on Bunch Palm Oil

Abstract

Xylanase (endoxylanase, EC 3.2.1.8) is a commercial enzyme that has been applied in the industrial production of fuel, food, textiles and paper. Xylanase was isolated from the culture supernatant of Bacillus sp. AQ-1 grown on Nakamura medium containing 0.5% powder bunch palm oil. The optimum pH and temperature of xylanase activity were pH 7.0 and 60 °C, respectively. The enzyme was purified by anion exchange chromatography using DEAE-Sepharose-Fast-Flow column and gel filtration chromatography using Sephacryl S-300 column. The results showed that purification of xylanase produced two forms of xylanase, which were identified as xylanase A and xylanase B. Xylanase A can be separated from xylanase B by ultrafiltration using a 30 kDa polyethersulfone membrane. The molecular weight of xylanase A and B were 15.7 and 57.7 kDa, respectively.

Production and biochemical characterization of partially purified cellulase-free, thermo-acidophilic endoxylanase from Lysinibacillus fusiformis strain TB7 using kolanut husk as feedstock

“…Low molecular weight xylanases within the range of 21 kDa as obtained in this study are particularly important in paper and pulp industries owing to the fact that smaller enzymes penetrate the fibre wall structure and works more efficiently in altering the pulp properties. Some researchers have reported similar xylanases with molecular weight within the range of 15.7–26.4 kDa ( Rahayu et al., 2008 ; Chi et al., 2010 ; Shrinivas et al., 2010 ). However, xylanases having higher molecular weights (40–100 kDa) has also been reported ( Verma et al., 2013 ).…”

“…It however exhibited reduction in activity at 70–90 °C with over remaining 400 U/mL ( Figure 4 ). An optimum temperature of 40 °C was reported for endoxylanase activity from Bacillus ( Rahayu et al., 2008 ). Interestingly, this purified L. fusiformis strain TB7 endoxylanase exhibited maximum thermal stability by retaining almost all its initial activity 94% at 40 °C while it retained over 70 and 80% activity after 120 min of pre-incubation at 50 and 60 °C, respectively.…”