Production and purification of lipase by a mutant strain ofRhizopus arrhizus

Chattopadhyay, Manojit; Banik, A. K.; Raychaudhuri, Sarmistha Sen

doi:10.1007/bf02816218

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…Examples include purification of lipase from R. arrhizus (Chattopadhyay et al, 1999), M. hiemalis f. hiemalis (Hiol et al, 1999) and A. carneus (Saxena et al, 2003). However, our initial assay showing activity using only MUF-butyrate as substrate indicated only esterase activity and was not sufficient to define the enzyme as a lipase (Lima et al, 2004).…”

Section: Discussionmentioning

confidence: 83%

Characterization of an extracellular lipase from the biocontrol fungus, Nomuraea rileyi MJ, and its toxicity toward Spodoptera litura

Supakdamrongkul

Bhumiratana

Wiwat

2010

Journal of Invertebrate Pathology

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Section: Discussionmentioning

confidence: 83%

Characterization of an extracellular lipase from the biocontrol fungus, Nomuraea rileyi MJ, and its toxicity toward Spodoptera litura

Supakdamrongkul

Bhumiratana

Wiwat

2010

Journal of Invertebrate Pathology

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“…An extracellular lipase from R. oryzae was purified 160-fold with 64% recovery [200] by acetone precipitation (80%), Sephadex G-100, and 1,260-fold with 22% recovery [201] by ammonium sulfate fractionation, sulfopropylSepharose, Sephadex G-75 and again on sulfopropyl-Sepharose. The lipase obtained from R. arrhizus was purified 720-fold with 42% recovery [202] by ammonium sulfate fractionation and Sephadex G-100 gel filtration. The purified enzyme has an apparent molecular mass of 67,000 gmol −1 on SDS-PAGE.…”

Section: Purificationmentioning

confidence: 99%

Overview of Fungal Lipase: A Review

Singh

Mukhopadhyay

2011

Appl Biochem Biotechnol

341

200

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Lipases (triacylglycerolacyl hydrolases, EC3.1.1.3) are class of enzymes which catalyze the hydrolysis of long-chain triglycerides. In this review paper, an overview regarding the fungal lipase production, purification, and application is discussed. The review describes various industrial applications of lipase in pulp and paper, food, detergent, and textile industries. Some important lipase-producing fungal genera include Aspergillus, Penicillium, Rhizopus, Candida, etc. Current fermentation process techniques such as batch, fed-batch, and continuous mode of lipase production in submerged and solid-state fermentations are discussed in details. The purification of lipase by hydrophobic interaction chromatography is also discussed. The development of mathematical models applied to lipase production is discussed with special emphasis on lipase engineering.

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“…A high concentration of Pb-adapted R. arrhizus strain M1 was developed and maintained as described previously (Bhattacharyya et al 2002;Chattopadhyay et al 1999). The WT strain was obtained from Bioengineering Division, Department of Chemical Engineering, University of Calcutta.…”

Section: Methodsmentioning

confidence: 99%

Modulation of enzyme activities of a lead-adapted strain of Rhizopus arrhizus during bioaccumulation of lead

Bhattacharyya

Pal²,

Basumajumdar

2009

Folia Microbiol

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Activity of some enzymes of a Pb-adapted strain of Rhizopus arrhizus augmented significantly during bioaccumulation of Pb from a chemically defined medium. The mycelium of a Pb-adapted strain exposed to 1 microg/mL Pb in a defined medium for 10 d at 28 +/- 2 degrees C exhibited, relative to a wild-type strain, increased activities of antioxidant enzymes, i.e. SOD, CAT and GPX and of enzymes like AP and PPO involved in defense against pathogens. Another response is a significant increase in the cellular thiol content after 4 d. The responses to Pb exposure thus include an increase in the antioxidant and anti-pathogen defense, and an increased metal-chelating ability.

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Production and purification of lipase by a mutant strain ofRhizopus arrhizus

Cited by 10 publications

References 10 publications

Characterization of an extracellular lipase from the biocontrol fungus, Nomuraea rileyi MJ, and its toxicity toward Spodoptera litura

Characterization of an extracellular lipase from the biocontrol fungus, Nomuraea rileyi MJ, and its toxicity toward Spodoptera litura

Overview of Fungal Lipase: A Review

Modulation of enzyme activities of a lead-adapted strain of Rhizopus arrhizus during bioaccumulation of lead

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