Production and properties of <i>Beauveria bassiana</i> conidia cultivated in submerged culture

Thomas, K. C.; Khachatourians, George G.; Ingledew, W. M.

doi:10.1139/m87-003

Cited by 113 publications

(56 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cultures were filtered twice through glass wool to remove mycelia, and the concentration of blastospores was determined by direct counting. Submerged conidia were produced in TKI broth using fructose as the carbon source as described previously (32). For all cell types, Miracloth-or glass woolfiltered cell suspensions were harvested by centrifugation (10,000 ϫ g, 15 min, 4°C), washed two times with sterile distilled H 2 O, and resuspended to the desired concentration as indicated below (typically 10 7 to 10 8 cells/ml).…”

Section: Methodsmentioning

confidence: 99%

Adhesion of the Entomopathogenic Fungus Beauveria ( Cordyceps ) bassiana to Substrata

Holder

Keyhani

2005

Appl Environ Microbiol

215

130

View full text Add to dashboard Cite

The entomopathogenic fungus Beauveria bassiana produces at least three distinct single-cell propagules, aerial conidia, vegetative cells termed blastospores, and submerged conidia, which can be isolated from agar plates, from rich broth liquid cultures, and under nutrient limitation conditions in submerged cultures, respectively. Fluorescently labeled fungal cells were used to quantify the kinetics of adhesion of these cell types to surfaces having various hydrophobic or hydrophilic properties. Aerial conidia adhered poorly to weakly polar surfaces and rapidly to both hydrophobic and hydrophilic surfaces but could be readily washed off the latter surfaces. In contrast, blastospores bound poorly to hydrophobic surfaces, forming small aggregates, bound rapidly to hydrophilic surfaces, and required a longer incubation time to bind to weakly polar surfaces than to hydrophilic surfaces. Submerged conidia displayed the broadest binding specificity, adhering to hydrophobic, weakly polar, and hydrophilic surfaces. The adhesion of the B. bassiana cell types also differed in sensitivity to glycosidase and protease treatments, pH, and addition of various carbohydrate competitors and detergents. The outer cell wall layer of aerial conidia contained sodium dodecyl sulfate-insoluble, trifluoroacetic acid-soluble proteins (presumably hydrophobins) that were not present on either blastospores or submerged conidia. The variations in the cell surface properties leading to the different adhesion qualities of B. bassiana aerial conidia, blastospores, and submerged conidia could lead to rational design decisions for improving the efficacy and possibly the specificity of entomopathogenic fungi for host targets.Under intensive study for use as a biopesticide, the entomopathogenic fungus Beauveria bassiana displays a broad host range and is able to target a number of diverse arthropod species (7,15,16,23,26,27,31). Strains of B. bassiana have been selected for control of insects and other arthropods that act as disease vectors, including mosquitoes and ticks (6, 22); crop pests, such as whiteflies, caterpillars, grasshoppers, and borers (5, 9, 21, 25, 36); and even ecologically hazardous, invading pests, such as fire ants and termites (4,8). The varied cuticles of these organisms represent the first barrier to the pathogen, and attachment of fungal propagules to the cuticle is the initial event in establishing mycosis. Air currents, dispersion via water droplets, and saprophytic growth over substrata inhabited by insects are considered the major routes for contact of fungal spores with host cuticles (2). Upon contact, fungal cells bind to the cuticle and initiate a developmental program that includes the production of specialized infection structures, such as germ tubes and penetrant hyphae (2,17). If the infection is successful, the fungus grows across the cuticle surface and penetrates the host cuticle to invade and proliferate within the hemolymph, which ultimately results in the death of the host.Fungal cell attachment to the cutic...

show abstract

Section: Methodsmentioning

confidence: 99%

Adhesion of the Entomopathogenic Fungus Beauveria ( Cordyceps ) bassiana to Substrata

Holder

Keyhani

2005

Appl Environ Microbiol

215

130

View full text Add to dashboard Cite

show abstract

“…Several authors made similar observations on morphological characteristics, growth, colony characteristics, etc. (Thomas et al 1987;Samson et al 1988;Glare and Inwood 1998;Fernandes et al 2009). …”

Section: Resultsmentioning

confidence: 99%

First Record of Entomopathogen Beauveria Bassiana (Bals.-Criv.) Vuill. On Zygogramma Bicolorata Pallister, A Biocontrol Agent of Parthenium Hysterophorus L.

Dubey¹,

Rây²,

Pandey³

2010

Journal of Plant Protection Research

View full text Add to dashboard Cite

Entomopathogenic fungi have great potential as biological control agents against insect pests. So they are being developed worldwide for the control of many pests of agricultural importance. But their effect on non-target insects, such as natural enemies has been a matter of great concern. Recently we came across an entomopathogen infecting a laboratory culture of Zygogramma bicolorata, a potential biocontol agent of noxious weed, Parthenium hysterophorus. The pathogen was isolated from the grubs and beetles and identified as Beauveria bassiana. In the present paper, the entomopathogen, B. bassiana is reported and described for the first time from the laboratory culture of Z. bicolorata.

show abstract

“…Para ello se utilizó la cepa de B. bassiana CCB-LE 262, la cual fue inoculada en diferentes medios líquidos y donde se evaluó su crecimiento durante las 24 h, 96 h y 216 h. Nuestros resultados indican que a las 216 h la mayor cantidad de esporas se obtuvo en el (Vega et al 2003, Lohse et al 2014. Thomas et al (1987) y Jenkins et al (1993, mencionan que las células hifales, también llamadas cuerpos hifales o blastosporas, suelen ser el único tipo de propágulos producidos en los esquemas de fermentación líquidos. Sin embargo, la patogenicidad de las blastosporas y esporas aéreas es la misma (Subramanian & Punamalai 2013).…”

Section: Introducción 76unclassified

Obtención De Esporas Y Biomasa De Beauveria Bassiana en Medios Líquidos Suplementados Con Eurysacca Melanocampta Y Peronospora Farinosa

Laynes

Acuña

Gutiérrez

2016

View full text Add to dashboard Cite

Keywords: Beauveria bassiana -Eurysacca melanocampta -quitin colloidal -laminarinThe aim of this investigation was to study the growth of Beauveria bassiana, grown in liquid media supplemented with minimum media with more artificial substrate (glucose, laminarin o colloidal chitin) and with minimum media with more natural substrate from E. melanocampta or Peronospora farinosa, for which we evaluated the number of spores and biomass. For this was used the CCB-LE 262 strain of B. bassiana, which was inoculated in different culture media and for which growth was evaluated after 24 h, 96 h and 216 h. Our results indicate that the highest number of spores was obtained in the artificial culture medium supplemented with laminarin 6 -1 (ML), with spores 67.80 x 10 ·mL , whereas the best natural media was that supplemented with 6 -1powder of E. melanocampta (M ), with 18.53 x 10 mL spores. The highest biomass was

show abstract

Production and properties of Beauveria bassiana conidia cultivated in submerged culture

Cited by 113 publications

References 0 publications

Adhesion of the Entomopathogenic Fungus Beauveria ( Cordyceps ) bassiana to Substrata

Adhesion of the Entomopathogenic Fungus Beauveria ( Cordyceps ) bassiana to Substrata

First Record of Entomopathogen Beauveria Bassiana (Bals.-Criv.) Vuill. On Zygogramma Bicolorata Pallister, A Biocontrol Agent of Parthenium Hysterophorus L.

Obtención De Esporas Y Biomasa De Beauveria Bassiana en Medios Líquidos Suplementados Con Eurysacca Melanocampta Y Peronospora Farinosa

Contact Info

Product

Resources

About