Production and properties of fibrinolytic enzyme in solid state cultures of Fusarium pallidoroseum

El-Aassar, Samy A.

doi:10.1007/bf00127431

Cited by 23 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Penicillium chrysogenum H9 and Cochliobolus lunatus were reported as source of fibrinolytic enzymes [48,49]. Fusarium pallidoroseum producing a metalloprotease having plasmin-like activity was documented [50]. Anticoagulant potential and fibrinolytic activity were reported from Aspergillus ochraceus [51], Oidiodendron flavum [52], Aspergillus strain KH 17 [53], and Aspergillus brasiliensis [54].…”

Section: Discussionmentioning

confidence: 99%

Anti‐MRSA and clot lysis activities of Pestalotiopsis microspora isolated from Dillenia pentagyna Roxb

2022

View full text Add to dashboard Cite

There is an urgent call to search for novel natural compounds against developing multidrug‐resistant microorganisms. The present work focuses on the characterization of a plant‐associated fungus having bioactivity against methicillin‐resistant Staphylococcus aureus (MRSA) strains. A fungal strain P31 was isolated from bark of Dillenia pentagyna and identified as Pestalotiopsis microspora. The maximum anti‐MRSA activity was observed from extract of P31 grown in sabouraud dextrose broth. The minimum inhibitory concentrations (MIC) values of P31 extract were 14 μg/ml for methicillin‐sensitive S. aureus (MSSA) and 32 μg/ml for MRSA strain, respectively. A crude P31 extract showed strong bactericidal activity by killing all treated MRSA cells within 24 h of treatment at their respective MIC value. A scanning electron microscopic study visualized morphological damage of MRSA cells. The membrane permeability of P31 extract‐treated MRSA cells gradually increased which caused release of internal cytoplasmic nucleic acids, proteins and potassium ions (K+) from cells suggesting cell lysis or leakage from cells. A very low concentration of P31 extract was able to inhibit biofilm formed by MRSA cells. Thin layer chromatographic separation followed by gas chromatography‐mass spectrometry analysis of the P31 extract revealed a number of antimicrobial compounds along with an anti‐MRSA compound 2,4‐di‐tert‐butylphenol. In addition, the P31 extract also showed in‐vitro human blood clot lysis activity at various concentrations. The clot lysis activity of P31 extract was found maximum at 500 µg/ml. These findings suggest that fungal isolate P31 has potential as a source of anti‐MRSA compounds useful in staph infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Anti‐MRSA and clot lysis activities of Pestalotiopsis microspora isolated from Dillenia pentagyna Roxb

2022

View full text Add to dashboard Cite

show abstract

“…The semipuri ed enzyme fractions were transferred to a diethyl amino ethyl cellulose ion-exchange chromatography (DEAE-Cellulose) column. The fractions (21)(22)(23)(24)(25)(26)(27) showed a recovery of 55.89% with speci c activity of 2581.6 U/mg protein and 6.37 puri cation fold. The SDS-PAGE electrophoresis of the puri ed brinolytic enzyme, con rming the existence of a single protein band (Fig.…”

Section: Puri Cation Of Brinolytic Enzyme From C Hawaiiensismentioning

confidence: 99%

Examination of thrombolytic and anticoagulant activities of purified fibrinolytic enzyme extracted from Cochliobolus hawaiiensis under solid state fermentation

Abu‐Tahon

Abdel-Mageed

Ghareib

et al. 2023

Preprint

View full text Add to dashboard Cite

Cochliobolus hawaiiensis Alcorn AUMC 8606 was chosen from the screened twenty fungal species as the potent producer of fibrinolytic enzyme on skimmed-milk agar plates. The greatest enzyme yield was attained when the submerged fermentation (SmF) conditions were optimized, and it was around (39.7 U/mg protein). Moreover, Upon optimization of fibrinolytic enzyme production under solid state fermentation (SSF), the maximum productivity of fibrinolytic enzyme was greatly increased recorded a bout (405 U/mg protein) on sugar cane bagasse. The yield of fibrinolytic enzyme by C. hawaiiensis under SSF was higher than that of SmF with about 10.20 fold. The purification procedures of fibrinolytic enzyme caused a great increase in its specific activity to 2581.6 U/mg protein with an overall yield of 55.89%, 6.37 purification fold and molecular weight of 35kDa. Maximal activity was recorded at pH 7 and 37oC. The enzyme showed the highest affinity towards Fibrin, with Vmax of 240 U/ml and an apparent Km value of 47.61 mmol. Mg2+ and Ca2+ moderately induced fibrinolytic activity, while Cu2+ and Zn2+ greatly suppressed the enzyme activity. The produced enzyme is categorized as serine protease and non metalloprotease due to the great suppression in its activity by using phenylmethylsulfonyl fluoride and thylenediamine-tetraacetat, respectively. The purified fibrinolytic enzyme showed efficient thrombolytic and antiplaetlet aggregation activities by completely prevention and dissolution of the blood clot which confirmed by microscopic examination and amelioration of blood coagulation assays. These findings suggested that the produced fibrinolytic enzyme is a promising agent in management of blood coagulation disorders

show abstract

“…Some of the preparations of micromycete' proteinases have successfully passed preclinical and clinical trials [52,53]. Effective fibrin lysis was found in proteinases from Fusarium oxyporum [54], F. pallidoroseum [55], Paecilomyces tenuipes [56], Mucor subtilissimus [57], Rhizopus chinensis [58] and Rhizomucor miehei [59]. Proteases with plasminogen activating activity were found in representatives of micromycetes of different systematic and ecological-trophic groups: Arthrobotrys longa [60], Tolypocladium inflatum [61], Rhizopus microsporus var.…”

Section: Microbial Fibrinolytic Enzymes: Production Status and Diversitymentioning

confidence: 99%

“…At the same time, the yield of enzymes in such a cultivation system increases significantly by 1.5 times and more [142]. For example, when cultivating Fusarium pallidoroseum on wheat bran, it was possible to obtain an increase in the secretion of fibrinolytic protease by 1.58 times [55], and when cultivating Aspergillus ochraceus on silica gel and vermiculite specific activity was 1.5-3.5 times higher than in a submerged culture [143,144]. The high impact in such types of cultivation on fibrinolytic activity optimisation effect different parameters such as time, inoculum ratio, moisture content and particle size.…”

Section: Streptomyces Radiopugnans_vitsd8mentioning

confidence: 99%

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

2021

View full text Add to dashboard Cite

Cardiac disorders such as acute myocardial infarction, embolism and stroke are primarily attributed to excessive fibrin accumulation in the blood vessels, usually consequential in thrombosis. Numerous methodologies including the use of anti-coagulants, anti-platelet drugs, surgical operations and fibrinolytic enzymes are employed for the dissolution of fibrin clots and hence ameliorate thrombosis. Microbial fibrinolytic enzymes have attracted much more attention in the management of cardiovascular disorders than typical anti-thrombotic strategies because of the undesirable after-effects and high expense of the latter. Fibrinolytic enzymes such as plasminogen activators and plasmin-like proteins hydrolyse thrombi with high efficacy with no significant after-effects and can be cost effectively produced on a large scale with a short generation time. However, the hunt for novel fibrinolytic enzymes necessitates complex purification stages, physiochemical and structural-functional attributes, which provide an insight into their mechanism of action. Besides, strain improvement and molecular technologies such as cloning, overexpression and the construction of genetically modified strains for the enhanced production of fibrinolytic enzymes significantly improve their thrombolytic potential. In addition, the unconventional applicability of some fibrinolytic enzymes paves their way for protein hydrolysis in addition to fibrin/thrombi, blood pressure regulation, anti-microbials, detergent additives for blood stain removal, preventing dental caries, anti-inflammatory and mucolytic expectorant agents. Therefore, this review article encompasses the production, biochemical/structure-function properties, thrombolytic potential and other surplus applications of microbial fibrinolytic enzymes.

show abstract

Production and properties of fibrinolytic enzyme in solid state cultures of Fusarium pallidoroseum

Cited by 23 publications

References 11 publications

Anti‐MRSA and clot lysis activities of Pestalotiopsis microspora isolated from Dillenia pentagyna Roxb

Anti‐MRSA and clot lysis activities of Pestalotiopsis microspora isolated from Dillenia pentagyna Roxb

Examination of thrombolytic and anticoagulant activities of purified fibrinolytic enzyme extracted from Cochliobolus hawaiiensis under solid state fermentation

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

Contact Info

Product

Resources

About