Production and Partial Characterization of Cellulases from <i>Trichoderma</i> sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil

Andrade, Jackeline Pereira; Bispo, Aline Simões da Rocha; Marbach, Phellippe Arthur Santos; Nascimento, Rodrigo Pires do

doi:10.4061/2011/167248

Cited by 29 publications

(17 citation statements)

References 23 publications

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“…This results contradicts the results published by Andrade et al (2011) and Delabona et al (2013), who reported optimum pH values at 3.0 for cellulases produced by A. fumigatus and Trichoderma sp. IS-05, respectively.…”

Section: Effect Of Temperature and Ph On The Cellulase Enzyme Activitycontrasting

confidence: 99%

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Oliveira

Maciel

Sancho

et al. 2016

Bioresour. Bioprocess.

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Background:The use of residues in industrial processing is expanding due to their low cost and abundant availability. Coconut shell is generated in large amounts in tropical areas due to the fruit processing and the coconut water consumption. In the present work, a new microbial strain was isolated from the coconut shell powder, molecularly identified as Melanoporia sp. CCT 7736 and applied for cellulase production in solid-state fermentation using the green coconut shell powder as substrate. Results:The complete production process was optimized. Fermentation time was only 24 h, and the enzyme produced presented maximal activity at neutral pH (6.5) and 60 °C. The maximal enzyme activity after extraction optimization was 7.5 IU/gds (international units of enzyme activity per gram of dry solid). For the enzyme extraction, the rotation velocity, the extraction time, the temperature, and the solvent volume (buffer) were optimized using response surface methodology (RSM). The best results for the enzyme extraction were obtained at 250 rpm (orbital shaker) at 30 °C using 13.79 mL of a sodium acetate buffer (200 mM) at pH 6.5 after 10 min. Delignification pretreatment was not necessary since this fungus strain was able to degrade the lignin. Conclusions:To the best of our knowledge, this work is the first report of cellulase production by Melanoporia sp. CCT 7736. Good results were obtained without the need for expensive pretreatment usually applied to lignocellulosic residues because the strain was isolated from coconut shell powder, and it is well adapted to this kind of substrate. The enzyme presented maximum activity at neutral pH instead of acidic pH as reported for the majority of industrial cellulases. The use of lignified coconut shell and the optimal pH at neutral values are the main advantages of the enzyme produced by Melanoporia sp. CCT 7736. In addition, the enzyme showed good stability during storage even at the crude broth and without any cryoprotection.

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Section: Effect Of Temperature and Ph On The Cellulase Enzyme Activitycontrasting

confidence: 99%

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Oliveira

Maciel

Sancho

et al. 2016

Bioresour. Bioprocess.

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“…MTCC 3922 presented optimal enzyme activity at 50 and 70°C, respectively. Studies involving endoglucanases from Trichoderma strains described optimal activity at pH and temperature between 3.0 to 5.5 and 50 to 65°C (Gashe, 1992;Sul et al, 2004;Andrade et al, 2011). Our results show that the optimal CMCase activity occurred in the 102C1 mutant strain at more acidic pH (3.6), and at a high temperature (66°C).…”

Section: Discussionsupporting

confidence: 52%

Production of thermophilic and acidophilic endoglucanases by mutant Trichoderma atroviride 102C1 using agro-industrial by-products

Oliveira¹,

Lima²,

Bon³

et al. 2016

Afr. J. Biotechnol.

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Many traditional mutagenic strategies have been used to improve cellulase production by microorganisms, especially fungi species. Trichoderma species are among cellulolytic fungi, those that have been most extensively studied, due to their efficient production of these enzymes. In the present study, N-methyl-N´-nitro-N-nitrosoguanidine (NTG) was used as mutagenic agent to obtain cellulolytic mutant from wild strain T. atroviride 676. After mutagenic procedures, two strains (102C1 and 104C2) were selected as promising cellulase-producing mutant. The effect of the carbon (sugarcane bagasse: SCB) and nitrogen (corn steep liquor: CSL) sources on endoglucanase production by the mutants 102C1 and 104C2 was studied using submerged cultivations at 28°C. Different concentrations of SCB and CSL were used and nine different media were generated. Mutant 102C1 showed the best results when using 2.5% SCB and 0.7% CSL. A central composite rotational design (CCRD) was performed to estimate optimal conditions of pH and temperature for endoglucanase activity of strain 102C1, which were pH 3.6 and temperature 66°C. The characterization of this acidophilic and thermophilic endoglucanase activity produced by the mutant strain 102C1 allows its use in biotechnological applications, particularly in the hydrolysis of agro industrial residues, such as SCB, for bioethanol production.

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“…Among the isolated fungi, Trichoderma sp. has been widely studied for cellulase production [ 29 ]. Members of the Aspergillus genera were found to be more prominent than the other moulds.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Palm Oil Mill Effluent by a Microbial Consortium Developed from Compost Soils

Nwuche

Aoyagi

Ogbonna

2014

International Scholarly Research Notices

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A method for the aerobic treatment of palm oil mill effluent (POME) was investigated in shake-flask experiments using a consortium developed from POME compost. POME was initially centrifuged at 4,000 g for 15 min and the supernatant was enriched with (NH4)2SO4 (0.5%) and yeast extract (0.25%) to boost its nitrogen content. At optimum pH (pH 4) and temperature (40°C) conditions, the chemical oxygen demand (COD) of the effluent decreased from 10,350 to 1,000 mg/L (90.3%) after 7 days. The total bacterial population determined by plate count enumeration was 2.4 × 106 CFU/mL, while the fungal count was 1.8 × 103 colonies/mL. Bacteria of the genera Pseudomonas, Flavobacterium, Micrococcus, and Bacillus were isolated, while the fungal genera included Aspergillus, Penicillium, Trichoderma, and Mucor. When the isolated species were each inoculated into separate batches of the raw effluent, both pH and COD were unchanged. However, at 75 and 50% POME dilutions, the COD dropped by 52 and 44%, respectively, while the pH increased from 4 to 7.53. POME treatment by aerobic method is sustainable and holds promising prospects for cushioning the environment from the problems associated with the use of anaerobic systems.

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Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil

Cited by 29 publications

References 23 publications

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Production of thermophilic and acidophilic endoglucanases by mutant Trichoderma atroviride 102C1 using agro-industrial by-products

Treatment of Palm Oil Mill Effluent by a Microbial Consortium Developed from Compost Soils

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