Production and optimization of polyhydroxybutyrate (PHB) from Bacillus megaterium as biodegradable plastic

Ahmady‐Asbchin, Salman; Rezaee, Hassan; Safari, Moein; Zamanifar, Pantea; Siyamiyan, Davood

doi:10.5281/zenodo.3711400

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…Likewise, the PHB biomass yield value (Y p/x) was 0.52 g/L*h. Other strains of Bacillus reported lower values, around 0.40 g/L*h [53,54]. The PHB volumetric productivity value (Qp) was 0.04 g/L*h, a value higher than reported by Ahmady-Asbchin et al [55] and Patel et al [56] using glucose as a carbon source. On the other hand, other Bacillus cereus were reported to have similar and lower values, utilizing 30 • C and 150 rpm in liquid fermentation [57][58][59].…”

Section: Optimization Of Phb Production and Kinetic Studiesmentioning

confidence: 85%

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

San Miguel-González,

Alemán-Huerta,

Martínez-Herrera

et al. 2024

Microorganisms

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Environmental pollution caused by petroleum-derived plastics continues to increase annually. Consequently, current research is interested in the search for eco-friendly bacterial polymers. The importance of Bacillus bacteria as producers of polyhydroxyalkanoates (PHAs) has been recognized because of their physiological and genetic qualities. In this study, twenty strains of Bacillus genus PHA producers were isolated. Production was initially evaluated qualitatively to screen the strains, and subsequently, the strain B12 or Bacillus sp. 12GS, with the highest production, was selected through liquid fermentation. Biochemical and molecular identification revealed it as a novel isolate of Bacillus cereus. Production optimization was carried out using the Taguchi methodology, determining the optimal parameters as 30 °C, pH 8, 150 rpm, and 4% inoculum, resulting in 87% and 1.91 g/L of polyhydroxybutyrate (PHB). Kinetic studies demonstrated a higher production within 48 h. The produced biopolymer was analyzed using Fourier-transform infrared spectroscopy (FTIR), confirming the production of short-chain-length (scl) polyhydroxyalkanoate, named PHB, and differential scanning calorimetry (DSC) analysis revealed thermal properties, making it a promising material for various applications. The novel B. cereus isolate exhibited a high %PHB, emphasizing the importance of bioprospecting, study, and characterization for strains with biotechnological potential.

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Section: Optimization Of Phb Production and Kinetic Studiesmentioning

confidence: 85%

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

San Miguel-González,

Alemán-Huerta,

Martínez-Herrera

et al. 2024

Microorganisms

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Polyhydroxyalkanoates Production from Cacao Fruit Liquid Residues Using a Native Bacillus megaterium Strain: Preliminary Study

Quintero-Silva,

Suárez-Rodríguez,

Gamboa-Suárez

et al. 2023

J Polym Environ

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Cacao mucilage exudates (CMEs), byproducts of cacao beans fermentation, are effluents rich in sugars and low-molecular-weight organic acids. In this study, we utilized CMEs to formulate culture media for the biosynthesis of polyhydroxyalkanoates (PHAs) using a native Bacillus megaterium strain (B2). Our investigation followed the adaptation process of B2 to the CMEs-based media, and we closely monitored the development of the microorganism in terms of consumption of acids and sugars. Upon successful adaptation to the CMEs-based media, we transferred the strain to a 4-L batch bioreactor to evaluate the impact of operational variables on PHAs production. We examined the microorganisms’ yield, productivity, and growth kinetics using the CME-based media. Our results showed a product yield of 0.98 g PHA/g carbon source, with an accumulation percentage of 57% and a productivity increase of 33% compared to PHA produced from residual glycerol using the same microorganism. The biopolymer's structure was confirmed to be of a polyhydroxybutyrate homopolymer (PHB) type using various molecular characterization techniques, including Matrix-Assisted Laser Desorption/Ionization mass spectrometry (MALDI-TOF-MS), infrared spectroscopy (IR), elemental composition (EC), ultraviolet–visible spectroscopy (UV–VIS), and thermal analysis (TGA, DSC). Graphical Abstract

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Recent updates to microbial production and recovery of polyhydroxyalkanoates

et al. 2023

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Production and optimization of polyhydroxybutyrate (PHB) from Bacillus megaterium as biodegradable plastic

Cited by 3 publications

References 9 publications

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

Alkaline-Tolerant Bacillus cereus 12GS: A Promising Polyhydroxybutyrate (PHB) Producer Isolated from the North of Mexico

Polyhydroxyalkanoates Production from Cacao Fruit Liquid Residues Using a Native Bacillus megaterium Strain: Preliminary Study

Recent updates to microbial production and recovery of polyhydroxyalkanoates

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