Production and optimization of ligninolytic enzymes by white rot fungus Schizophyllum commune IBL-06 in solid state medium banana stalks

Abstract

The present study deals with production of ligninolytic enzymes from an indigenous white rot fungus Schizophyllum commune IBL-06 by using banana stalk as substrate through the process of solid state fermentation. The production process was further improved by optimizing a number of physical parameters such as incubation time, moisture level, pH, temperature, inoculums size and nutritional factors (carbon and nitrogen ratio, mediators and metal ions). By optimization of different parameters, the maximum activities of enzymes synthesized by S. commune IBL-06 were observed as 3745 IU/ml for manganese peroxidase (MnP), 2700 IU/ml for lignins peroxidase (LiP) and 345 IU/ml for laccase after 3 days incubation at рH 4.5 and 35°C temperature with 3 ml inoculum size, 60% moisture content, 20:1 C:N ratio (glucose and ammonium nitrate as carbon and nitrogen supplements), 1ml of 1mM MnSO 4 as mediator, and 1ml of 1mM MgSO 4 .7H 2 O 2. This High activities of ligninolytic enzymes produced by the fungus suggest its potential for commercial scale production of these enzymes for diverse industrial applications.

The agricultural waste utilization for exploration of the extracellular enzyme potential of three novel white-rot Basidiomycetes: Fomes fomentarius TMF2, Schizophyllum commune TMF3 and Bjerkandera adusta TMF1

“…In the study of Zhu and coworkers no laccase activity was detected for S. commune during 30 days SSF on Jerusalim artichoke stalk [13]. However, literature data also reported higher values of laccase activity under optimized conditions [35].…”

Biodegradation of Straw Lignin by Culture of the Fungus Schizophyllum Commune Isolated From the Tree Surface

“…Such physico-chemical and mechanical pre-treatment methods such as: acid, alkali and steam explosion are not preferred for several reasons: a) the structure of lignin changes; b) toxic emissions; c) high financial costs [9,10] . It can be said that the biological method of lignin decomposition is an alternative method of pre-treatment of wood and non-wood raw materials due to environmental safety and economic benefits.…”

“…Такие физико-химические и механические методы предварительной обработки как кислотная, щелочная и применение парового взрыва не являются предпочтительными по нескольким причинам: изменение структуры лигнина, токсические выброс, большие финансовые расходы [9,10]. Можно сказать, что биологический метод разложения лигнина является альтернативным методом предварительной обработки древесного и недревесного сырья благодаря экологической безопасности и экономической выгоды.…”