Production and evaluation of monoclonal antibodies directed against the K88 fimbrial adhesin produced by Escherichia coli enterotoxigenic for piglets

Thorns, C. J.; Boarer, C. D. H.; Ja, Morris

doi:10.1016/s0034-5288(18)30779-3

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…Pigs were typed by the microscopic adhesion test using three strains of F4 E. coli obtained from the Central Veterinary Laboratory (Weybridge, Surrey, UK). They carried the designations E68 I (O141:K85ab:F4ab), G4 (O45:K ‘E65’:F4ac) and Guinée (O8:K87:F4ad) (Thorns et al. 1987).…”

Section: Phenotyping and Marker Polymorphismsmentioning

confidence: 99%

Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acR

Python

Jörg

Neuenschwander

et al. 2005

J Animal Breeding Genetics

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Susceptibility to enterotoxigenic Escherichia coli with fimbriae F4ac is dominantly inherited in the pig. A three-generation pedigree was created to refine the position of F4acR on chromosome 13 comprising 202 pigs: eight parents, 18 F1 and 176 F2 pigs. The 17-point analysis indicates that F4acR lies between Sw207 and S0283. Recombinant offspring specify that the most probable order is Sw207-S0075-F4acR-Sw225-S0283. We observed six phenotypes for the three fimbrial variants F4ab, F4ac and F4ad. The two missing phenotypes F4abR-/F4acR+/F4adR+ and F4abR-/F4acR+/F4adR- indicate that pigs susceptible to F4ac are always susceptible to F4ab. Furthermore, a weak and a strong adhesion of F4ab and F4ad bacteria was observed. The weak receptor F4abR (F4abRw) was present only in pigs devoid of the receptor F4acR (F4abR+/F4acR-). In contrast, in pigs with the phenotype F4abR+/F4acR+, F4ab bacteria adhered to the majority of enterocytes. F4abRw constitutes a frequently observed phenotype whose inheritance is still unclear. Strong adhesion of F4ab and F4ac bacteria is most likely influenced by the same receptor that we name F4bcR. The number of F4ad bacteria that adhered to enterocytes was very variable in the adhesion test. Moreover, expression of F4adR was independent of age. Our segregation analyses indicated a dominant inheritance of F4adR, although the number of susceptible pigs was smaller than expected. We examined four genes as candidates for the F4acR locus: the transferrin receptor gene (TFRC) and three genes members of the glucosyl/galactosyltransferase family (B3GnT5, B3GALT3 and B4GALT4). Comparison of sequences from resistant and homozygous susceptible F4ac pigs did not reveal any causative single nucleotide polymorphism in the four genes. Two silent mutations at the positions 295 (C/T) and 313 (T/C) in B3GALT3 were found. Using the somatic cell hybrid panel, B3GnT5 and B3GALT3 were assigned to the chromosomal region SSC13q23-q41. No mutations were found in the cDNA sequences of these genes associated with the F4acR genotypes.

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Section: Phenotyping and Marker Polymorphismsmentioning

confidence: 99%

Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acR

Python

Jörg

Neuenschwander

et al. 2005

J Animal Breeding Genetics

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“…The two strains of E. coli used were obtained from the Central Veterinary Laboratory, Weybridge, Surrey GB. They carried the designations E68 I (O141: K85ab: F4ab) and G4 (O45: K ‘E65’: F4ac) (Thorns et al . 1987).…”

Section: Microscopic Enterocyte Adhesion Testmentioning

confidence: 99%

Fine‐mapping of the intestinal receptor locus for enterotoxigenic Escherichia coli F4ac on porcine chromosome 13

et al. 2002

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The aim of this study was to refine the localization of the receptor locus for fimbriae F4ac. Small intestinal enterocyte preparations from 187 pigs were phenotyped by an in vitro adhesion test using two strains of Escherichia coli representing the variants F4ab and F4ac. The three-generation pedigree comprised eight founders, 18 F1 and 174 F2 animals, for a total of 200 pigs available for the linkage analysis. Results of the adhesion tests on 171 F2 pigs slaughtered at 8 weeks of age show that 23.5% of the pigs were adhesive for F4ab and non-adhesive for F4ac (phenotype F4abR+/F4acR-; R means receptor). Pigs of this phenotype were characterized by a weak adhesion receptor for F4ab. No pigs were found expressing only F4acR and lacking F4abR. Receptors for F4ab and F4ac (F4abR+/F4acR+) were expressed by 54.5% of the pigs. Animals of this phenotype strongly bound both F4ab and F4ac E. coli. In the segregation study, the serum transferrin (TF) gene and 10 microsatellites on chromosome 13 were linked with F4acR (recombination fractions (theta) between 0.00 and 0.11 and lod score values (Z) between 11.4 and 40.4). The 11-point analysis indicates the F4acR locus was located in the interval S0068-Sw1030 close to S0075 and Sw225, with recombination fractions (theta) of 0.05 between F4acR and S0068, 0.04 with Sw1030, and 0.00 with S0075 and Sw225. The lack of pigs displaying the F4abR-/F4acR+ phenotype and the presence of two phenotypes for F4abR (a strong receptor present in phenotype F4abR+/F4acR+ and a weak receptor in phenotype F4abR+/F4acR-) led us to conclude that the receptor for F4ac binds F4ab bacteria as well, and that it is controlled by one gene localized between S0068 and Sw1030 on chromosome 13.

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“…The enterocytes were prepared and the MAT performed according to Vögeli et al (1996) and Python et al (2002). Escherichia coli test strains E68I (O 141: K 85ab: F4ab), G4 (O 45: K (E 65): F4ac) and Guinée (O 8: K 87: F4ad) were procured from the Veterinary Laboratory Agency Weybridge, Surrey GB (Thorns et al, 1987). Expression of the fimbriae was checked serologically and the variant specificity by PCR (Alexa et al, 2001).…”

Section: Pigsmentioning

confidence: 99%

Inheritance of porcine receptors for enterotoxigenic Escherichia coli with fimbriae F4ad and their relation to other F4 receptors

Rampoldi

Bertschinger

Bürgi

et al. 2014

Animal

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Enteric Escherichia coli infections are a highly relevant cause of disease and death in young pigs. Breeding genetically resistant pigs is an economical and sustainable method of prevention. Resistant pigs are protected against colonization of the intestine through the absence of receptors for the bacterial fimbriae, which mediate adhesion to the intestinal surface. The present work aimed at elucidation of the mode of inheritance of the F4ad receptor which according to former investigations appeared quite confusing. Intestines of 489 pigs of an experimental herd were examined by a microscopic adhesion test modified in such a manner that four small intestinal sites instead of one were tested for adhesion of the fimbrial variant F4ad. Segregation analysis revealed that the mixed inheritance model explained our data best. The heritability of the F4ad phenotype was estimated to be 0.7 ± 0.1. There are no relations to the strong receptors for variants F4ab and F4ac. Targeted matings allowed the discrimination between two F4ad receptors, that is, a fully adhesive receptor (F4adR FA ) expressed on all enterocytes and at all small intestinal sites, and a partially adhesive receptor (F4adR PA ) variably expressed at different sites and often leading to partial bacterial adhesion. In pigs with both F4ad receptors, the F4adR PA receptor is masked by the F4adR FA . The hypothesis that F4adR FA must be encoded by at least two complementary or epistatic dominant genes is supported by the Hardy-Weinberg equilibrium statistics. The F4adR PA receptor is inherited as a monogenetic dominant trait. A comparable partially adhesive receptor for variant F4ab (F4abR PA ) was also observed but the limited data did not allow a prediction of the mode of inheritance. Pigs were therefore classified into one of eight receptor phenotypes: A1 (F4abR FA /F4acR + /F4adR FA ); A2 (F4abR FA /F4acR + /F4adR PA ); B (F4abR FA /F4acR + / F4adR − ); C1 (F4abR PA /F4acR − /F4adR FA ); C2 (F4abR PA /F4acR − /F4adR PA ); D1 (F4abR − /F4acR − /F4adR FA ); D2 (F4abR − /F4acR − / F4adR PA ); E (F4abR − /F4acR − /F4adR − ).

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Production and evaluation of monoclonal antibodies directed against the K88 fimbrial adhesin produced by Escherichia coli enterotoxigenic for piglets

Cited by 11 publications

References 11 publications

Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acR

Inheritance of the F4ab, F4ac and F4ad E. coli receptors in swine and examination of four candidate genes for F4acR

Fine‐mapping of the intestinal receptor locus for enterotoxigenic Escherichia coli F4ac on porcine chromosome 13

Inheritance of porcine receptors for enterotoxigenic Escherichia coli with fimbriae F4ad and their relation to other F4 receptors

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