Production and Cleaning of Spores of Putrefactive Anaerobe 36791

Production of Clostridial Spores

“…This seems to have been an arbitrary choice in most cases, but the few studies that have been made of relative sporulation at several pH levels tend to confirm that the optimum for these species is between 7.0 and 8.0. Brown et al (1957) confirmed that the optimum for PA 3679 was in this range. I n a 5% trypticase medium they noted maximum sporulation a t pH 7.2 and that only about 10% of the cells sporulated in this medium a t pH 6.0 and pH 8.0.…”

“…It should be noted that good sporulation of C1. parabotulinum 62A (Costilow, 1962) and of PA 3679 (Brown et al, 1957) has been reported in casein hydrolysate unsupplemented with any other peptone.…”

“…These observations suggest that a t low peptone concentrations, sporulation of clostridia might be improved by the addition of a dilute mixture of salts such as employed by Mager et al (1954) or Fuchs & Bonde (1957). The stimulatory action of ammonium salts that has been occasionally eported (Leifson, 1931;Brown et al, 1957) is almost certainly an accessory nitrogen source.…”

“…A survey of publications relating to the production of clostridial spores in peptone media, reveals a very wide range of suggested concentrations. Trypticase concentrations ranging from 10% (Brown, Ordal & Halvorson, 1957) to 1 6 % (supplemented with l -O~o of peptone (Zoha & Sadoff, 1958)) have been used successfully for the production of spores of the identical NCA strain of PA 3679. The extremes which have been used for preparing spores of various clostridial species are compared in Table 4 on the basis of their total nitrogen content.…”

Production and characterization of pureClostridiumspore suspensions

“…The biggest challenge for the production of pure Clostridium spore suspensions is its typically unsynchronized growth habits. Most Clostridium cultures contain all possible cell forms: young vegetative cells, cells in various stages of sporulation, free spores, and germinated spores (Brown et al 1957;Collier 1957;Powers 1967). Past efforts to harvest clean spores from such a mixture have been unsuccessful (Collier 1957;Zoha and Sadoff 1958).…”