Production and Characterization of β-Glucan Type Oligomer Produced with Enzymatic Hydrolysis of Capsosiphon fulvescens

Abstract

β-Glucan type oligomers which have angiotensin I converting enzyme (ACE) inhibitory activity were isolated and characterized from Capsosiphon fulvescens. After C. fulvescens was hydrolysis with Alcalase at 50 o C, supernatant was harvested and separated with ultrafiltration membrane (MWCO 2 kDa). Oligomers which were less than 2 kDa of molecular weight were harvested for characterization. The nutrient composition of Alcalase hydrolysate was 89.9% carbohydrate, 4.2% protein and 5.9% sulfate. After ultrafiltration, the nutrient composition of oligomers was changed to 99.88% carbohydrate, 0.07% protein and 0.05% sulfate. The carbohydrate composition of oligomer was glucose (97.2%) and mannose (1.5%). The ACE inhibitory activities of Alcalase hydrolysate and oligomer were 72.1% and 82%, respectively. The molecular weight of oligomer was about 1 kDa. The oligomer was analyzed with FT-IR, 1 H-NMR and methylation. The oligomers were β-1,3-glucans with β-(1,3)-linked glucose units.

Polysaccharide from Hizikia Fusiformis Enhances the Immunomodulatory Activity of Macrophages

“…The procedure was similar to the previous work. 11) After grinding freeze dried H. fusiformis in a mixer, 2 g of powder was digested and extracted at 50°C with 100 uL of viscozyme (in 100 mL of 0.1 M sodium acetate buffer solution pH 4.5) for six hours in a shaking incubator (200 rpm). After digestion, the viscozyme was denatured at 100°C for 15 min and supernatant was harvested with centrifuge (10,000 g for 10 min).…”