Production and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway

Meng, De-Chuan; Shi, Zhenyu; Wu, Ling-Ling; Zhou, Qing; Wu, Qiong; Chen, Jinchun; Chen, Guoqiang

doi:10.1016/j.ymben.2012.04.003

Cited by 113 publications

(47 citation statements)

References 57 publications

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“…droxyvalerate-co-4-hydroxybutyrate) P(3HB-co-3HV --co-4HB), have been produced by batch fermentation. [40][41][42][43][44][45][46][47][48][49][50] Production of mcl-PHAs and other interesting PHA copolymers including scl-copolymers and scl-mcl copolymers has also been reported using unconventional substrates, such as lignocellulosics e.g., grass mass hydrolysate, rice bran, rapeseed meal hydrolysate, and industrial waste e.g., whey, biodiesel waste, vinnase etc. 35,48,[51][52][53][54][55][56] Utilization of waste whey for PHA production is particularly useful as only 50 % of the whey produced is used for production of food ingredients and animal feed, while the rest remains as a pollutant owing to its high biological oxygen demand.…”

Section: Ta B L E 1 -Summary Of Results Of Batch Fermentation For Promentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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Section: Ta B L E 1 -Summary Of Results Of Batch Fermentation For Promentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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“…NMR is broadly used for saturated and unsaturated PHA analysis. Functional groups such as methane protons, methylene protons, -CH=CH-can be identified from both NMR spectra while microstructures like 3-hydroxypropionate (3HP) and 4-hydroxybutyrate (4HB) can be obtained by analyzing both 1 H-NMR and 13 C-NMR spectra [156,169]. Quantitative estimation of PHA monomers can also be performed with NMR using the intensity ratio of the signals [156].…”

Section: Monomeric Composition and Distributionmentioning

confidence: 99%

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

et al. 2014

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“…3) (45). Remarkably, poly(3HP-co-4HB) films become more transparent, with increases in 4HB content up to 67 mol%, whereas higher 4HB amounts lowered the transparency (45).…”

Section: Chemical and Physical Propertiesmentioning

confidence: 97%

“…Depending on the medium and substrate supply, the achieved poly(3HP-co-4HB) content ranged between 24.7% and 41.7% (wt PHA /wt CDW ) with 4HB fractions from 0.5 to 2.0 mol% (Table 3) (28). To improve 4HB incorporation, a specific 4-hydroxybutyrate-CoA transferase (OrfZ) from C. kluyveri was coexpressed in the aforementioned strain (45). When the recombinant strain was cultivated in the presence of 1,3PD and 1,4BD, levels of poly(3HP- .…”

Section: Hp-containing Copolymersmentioning

confidence: 99%

“…It has been shown that PHB-b-PHBV synthesized by R. eutropha prevents polymer aging (51). Meng et al cultivated their recombinant E. coli strain in the presence of 1,3PD and 1,4BD to accumulate poly(3HP-co-4HB) (45). When the substrates were provided consecutively in the medium beginning with 1,4BD, cells synthesized at levels of 16% (wt PHA /wt CDW ) poly(3HP-b-36.7 mol% 4HB) (P PHA , 23.9 mg PHA liter Ϫ1 h Ϫ1 ) or 21% (wt PHA /wt CDW ) poly(3HP-b-28.5 mol% 4HB) (P PHA , 15.8 mg PHA liter Ϫ1 h Ϫ1 ), depending on the substrate concentration (52).…”

Section: Hp-containing Block Polymersmentioning

confidence: 99%

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Poly(3-Hydroxypropionate): a Promising Alternative to Fossil Fuel-Based Materials

Andreeßen

Taylor

Steinbüchel

2014

Appl Environ Microbiol

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bPolyhydroxyalkanoates (PHAs) are storage compounds synthesized by numerous microorganisms and have attracted the interest of industry since they are biobased and biodegradable alternatives to fossil fuel-derived plastics. Among PHAs, poly(3-hydroxypropionate) [poly(3HP)] has outstanding material characteristics and exhibits a large variety of applications. As it is not brittle like, e.g., the best-studied PHA, poly(3-hydroxybutyrate) [poly(3HB)], it can be used as a plasticizer in blends to improve their properties. Furthermore, 3-hydroxypropionic acid (3HP) is considered likely to become one of the new industrial building blocks, and it can be obtained from poly(3HP) by simple hydrolysis. Unfortunately, no natural organism is known to accumulate poly(3HP) so far. Thus, several efforts have been made to engineer genetically modified organisms capable of synthesizing the homopolymer or copolymers containing 3HP. In this review, the achievements made so far in efforts to obtain biomass which has accumulated poly(3HP) or 3HP-containing copolymers, as well as the properties of these polyesters and their applications, are compiled and evaluated.

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Production and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway

Cited by 113 publications

References 57 publications

Strategies for Large-scale Production of Polyhydroxyalkanoates

Strategies for Large-scale Production of Polyhydroxyalkanoates

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

Poly(3-Hydroxypropionate): a Promising Alternative to Fossil Fuel-Based Materials

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